scholarly journals Crosslinked chitosan: Its physical properties and the effects of matrix stiffness on chondrocyte cell morphology and proliferation

2005 ◽  
Vol 75A (3) ◽  
pp. 742-753 ◽  
Author(s):  
Anuradha Subramanian ◽  
Hsin-Yi Lin
Keyword(s):  
Physical Properties ◽  
Cell Morphology ◽  
Matrix Stiffness ◽  
Crosslinked Chitosan

Alginate dependent changes of physical properties in 3D bioprinted cell-laden porous scaffolds affect cell viability and cell morphology

2019 ◽  
Vol 14 (6) ◽  
pp. 065009 ◽  
Author(s):  
Jianhua Zhang ◽  
Esther Wehrle ◽  
Jolanda R Vetsch ◽  
Graeme R Paul ◽  
Marina Rubert ◽  
...  
Keyword(s):  
Cell Viability ◽  
Physical Properties ◽  
Cell Morphology ◽  
Porous Scaffolds ◽  
Affect Cell Viability

scholarly journals Incorporation of Fluconazole and Ocimum Sanctum Oil in Soft Denture Liners to Treat Biofilms of Candida albicans Associated with Denture Stomatitis

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Aamir Zahid Godil ◽  
Diksha Bhagat ◽  
Parijat Das ◽  
Ashi Ilyas Kazi ◽  
Ramandeep Dugal ◽  
...  
Keyword(s):  
Physical Properties ◽  
Cell Morphology ◽  
Antifungal Agents ◽  
Surface Hardness ◽  
Ocimum Sanctum ◽  
Denture Stomatitis ◽  
Denture Liners ◽  
And Control ◽  
Prolonged Drug Release

Purpose: To investigate the In Vitro activity of incorporated antifungal agents like Fluconazole and Ocimum sanctum oil (Tulsi) in the denture soft liners to reduce the risks associated with the biofilms of Candida albicans.Materials and Methods: In the current study, the minimum inhibitory concentration (MIC) of two antifungal agents namely Fluconazole and O. sanctum (Tulsi oil) against C. albicans (ATCC 10231) was evaluated to examine their effect in reducing the count of candida and its biofilms. Analysis of physical properties such as surface roughness and hardness of soft denture liners (test and control) were also performed. The effect of both antifungal agents was also observed on cell morphology of candida cells using scanning electron microscopy (SEM).Results: Studies confirmed MIC value for fluconazole and O. sanctum oil as 600 and 400 µg/ml respectively. Surface hardness and roughness of soft denture material (test) remained unaltered. Finally, SEM studies also proved the effectiveness of incorporated antifungal agents on the cell morphology of C. albicans at their respective MIC values.Conclusions: This approach allows the prolonged drug release in the oral cavity which simultaneously treats the injured denture bearing tissues and also the infection, biofilms of candida without compromising on their physical properties. These studies are significant and have tremendous medicinal and therapeutic relevance.

scholarly journals Osteogenesis-Related Behavior of MC3T3-E1 Cells on Substrates with Tunable Stiffness

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Yingying Zhang ◽  
Yanghui Xing ◽  
Jian Li ◽  
Zhiqiang Zhang ◽  
Huiqin Luan ◽  
...  
Keyword(s):  
Elastic Modulus ◽  
Osteogenic Differentiation ◽  
Cell Morphology ◽  
Subcellular Location ◽  
Substrate Stiffness ◽  
Matrix Stiffness ◽  
Osteogenic Markers ◽  
Enhanced Expression ◽  
Extracellular Matrix Stiffness ◽  
Cells Cultured

Osteogenic differentiation of cells has considerable clinical significance in bone defect treatment, and cell behavior is linked to extracellular matrix stiffness. This study aimed to determine how matrix stiffness affects cell morphology and subsequently regulates the osteogenic phenotype of osteogenesis precursor cells. Four PDMS substrates were prepared with stiffness corresponding to the elastic modulus ranging from 0.6 MPa to 2.7 MPa by altering the Sylgard 527 and Sylgard 184 concentrations. MC3T3-E1 cells were cultured on the matrices. Cell morphology, vinculin expression, and key osteogenic markers, Col I, OCN, OPN, and calcium nodule, were examined. The activity and expression level of Yes-associated protein (YAP) were evaluated. Results showed that cell spreading exhibited no correlation with the stiffness of matrix designed in this paper, but substratum stiffness did modulate MC3T3-E1 osteogenic differentiation. Col I, OPN, and OCN proteins were significantly increased in cells cultured on soft matrices compared with stiff matrices. Additionally, cells cultured on the 1:3 ratio matrices had more nodules than those on other matrices. Accordingly, cells on substrates with low stiffness showed enhanced expression of the osteogenic markers. Meanwhile, YAP expression was downregulated on soft substrates although the subcellular location was not affected. Our results provide evidence that matrix stiffness (elastic modulus ranging from 0.6 MPa to 2.7 MPa) affects the osteogenic differentiation of MC3T3-E1, but it is not that “the stiffer, the better” as showed in some of the previous studies. The optimal substrate stiffness may exist to promote osteoblast differentiation. Cell differentiation triggered by the changes in substrate stiffness may be independent of the YAP signal. This study has important implications for biomaterial design and stem cell-based tissue engineering.

scholarly journals OC-157 Physiological changes in matrix stiffness modulate hepatic progenitor cell morphology, proliferation and differentiation

Gut ◽  
2012 ◽  
Vol 61 (Suppl 2) ◽  
pp. A67.3-A68 ◽  
Author(s):  
T T Gordon-Walker ◽  
J Schrader ◽  
L Boulter ◽  
A J Robson ◽  
S J Forbes ◽  
...  
Keyword(s):  
Cell Morphology ◽  
Progenitor Cell ◽  
Physiological Changes ◽  
Matrix Stiffness ◽  
Hepatic Progenitor Cell ◽  
Proliferation And Differentiation

scholarly journals Erratum to: Incorporation of Fluconazole and Ocimum Sanctum Oil in Soft Denture Liners to Treat Biofilms of Candida albicans Associated with Denture Stomatitis

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Ramandeep Dugal
Keyword(s):  
Surface Roughness ◽  
Physical Properties ◽  
Cell Morphology ◽  
Antifungal Agents ◽  
Inhibitory Concentration ◽  
Surface Hardness ◽  
Ocimum Sanctum ◽  
Denture Stomatitis ◽  
Denture Liners ◽  
Scanning Electron

Purpose: To investigate the In Vitro activity of incorporated antifungal agents like Fluconazole and Ocimum sanctum oil (Tulsi) in the denture soft liners to reduce the risks associated with the biofilms of Candida albicans.Materials and Methods: In the current study, the minimum inhibitory concentration (MIC) of two antifungal agents namely Fluconazole and O. sanctum (Tulsi oil) against C. albicans (ATCC 10231) was evaluated to examine their effect in reducing the count of candida and its biofilms. Analysis of physical properties such as surface roughness and hardness of soft denture liners (test and control) were also performed. The effect of both antifungal agents was also observed on cell morphology of candida cells using scanning electron microscopy (SEM).Results: Studies confirmed MIC value for fluconazole and O. sanctum oil as 600 and 400 µg/ml respectively. Surface hardness and roughness of soft denture material (test) remained unaltered. Finally, SEM studies also proved the effectiveness of incorporated antifungal agents on the cell morphology of C. albicans at their respective MIC values.Conclusions: This approach allows the prolonged drug release in the oral cavity which simultaneously treats the injured denture bearing tissues and also the infection, biofilms of candida without compromising on their physical properties. These studies are significant and have tremendous medicinal and therapeutic relevance.        Keywords: Antifungal Agents; Biofilms; Candida; Scanning Electron Microscope; Soft Liners; Hardness; Surface Roughness; Minimal Inhibitory Concentration; Denture Stomatitis.

scholarly journals Relationship of Matrix Stiffness and Cell Morphology in Regulation of Osteo Genesis and Adipogenesis of BMSCs

2021 ◽  
Author(s):  
Yutong Guo ◽  
Yini Qiao ◽  
Shuqi Quan ◽  
Cai Yang ◽  
Juan Li
Keyword(s):  
Tissue Culture ◽  
Cell Morphology ◽  
Actin Polymerization ◽  
Nuclear Translocation ◽  
Cell Spreading ◽  
The Other ◽  
Matrix Stiffness ◽  
Robust Regulation ◽  
Relationship Of ◽  
Rat Bone

Abstract Backgrounds: Both matrix stiffness and cell morphology have been found as important factors directing MSCs (mesenchymal stem cells) differentiation, but cells also spontaneously adapt their morphology under matrix stiffness stimulation. This study aimed to investigate the interplay of cell morphology and matrix stiffness on osteogenesis and adipogenesis of rBMSCs(rat bone BMSCs) on 2D substrates. Methods and Results: First, we modulated MSCs morphology through different fibronectin (FN) concentrations on tissue culture plates (TCPs). We found FN promoted and osteogenesis of BMSCs while suppressing adipogenesis, mediated by FN-induced F-actin polymerization and cell spreading. Based on these findings, we modulated BMSCs morphology on 0.5 kPa and 32 kPa CytoSoft® plates through FN concentrations. We found BMSCs on 0.5 kPa substrates coated with 300μg/ml of FN manifested similarly spreading morphology with cells on 32 kPa substrates coated with 100 μg/ml of FN, and cells in both groups dominantly commit osteogenesis. On the other hand, BMSCs on 32 kPa substrates coated with 30μg/ml of FN manifested similarly restricted morphology with cells in on 0.5 kPa substrates with 100μg/ml of FN, and in both groups cells mainly commit adipogenesis. Immunofluorescence staining indicated YAP/TAZ mainly located in cytoplasm when cells exhibited restricted morphology on stiff matrices, while exhibiting significant nuclear translocation when cells spread on soft matrices. Conclusions: Cell morphology overrode effects of matrix stiffness on BMSCs differentiation through more robust regulation of YAP/TAZ. Matrix stiffness depended on cell morphology to regulate osteogenesis and adipogenesis of BMSCs.

scholarly journals Cell Shape and Matrix Stiffness Impact Schwann Cell Plasticity via YAP/TAZ and Rho GTPases

2021 ◽  
Vol 22 (9) ◽  
pp. 4821
Author(s):  
Zhenyuan Xu ◽  
Jacob A. Orkwis ◽  
Greg M. Harris
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Cell Morphology ◽  
Rho Gtpases ◽  
Cell Shape ◽  
Peripheral Nerve Regeneration ◽  
Critical Role ◽  
Matrix Stiffness ◽  
Associated Proteins

Schwann cells (SCs) are a highly plastic cell type capable of undergoing phenotypic changes following injury or disease. SCs are able to upregulate genes associated with nerve regeneration and ultimately achieve functional recovery. During the regeneration process, the extracellular matrix (ECM) and cell morphology play a cooperative, critical role in regulating SCs, and therefore highly impact nerve regeneration outcomes. However, the roles of the ECM and mechanotransduction relating to SC phenotype are largely unknown. Here, we describe the role that matrix stiffness and cell morphology play in SC phenotype specification via known mechanotransducers YAP/TAZ and RhoA. Using engineered microenvironments to precisely control ECM stiffness, cell shape, and cell spreading, we show that ECM stiffness and SC spreading downregulated SC regenerative associated proteins by the activation of RhoA and YAP/TAZ. Additionally, cell elongation promoted a distinct SC regenerative capacity by the upregulation of Rac1/MKK7/JNK, both necessary for the ECM and morphology changes found during nerve regeneration. These results confirm the role of ECM signaling in peripheral nerve regeneration as well as provide insight to the design of future biomaterials and cellular therapies for peripheral nerve regeneration.

The Photometric Properties of the Ap Stars

1976 ◽  
Vol 32 ◽  
pp. 365-377 ◽  
Author(s):  
B. Hauck
Keyword(s):  
Physical Properties ◽  
Ap Stars

The Ap stars are numerous - the photometric systems tool It would be very tedious to review in detail all that which is in the literature concerning the photometry of the Ap stars. In my opinion it is necessary to examine the problem of the photometric properties of the Ap stars by considering first of all the possibility of deriving some physical properties for the Ap stars, or of detecting new ones. My talk today is prepared in this spirit. The classification by means of photoelectric photometric systems is at the present time very well established for many systems, such as UBV, uvbyβ, Vilnius, Geneva and DDO systems. Details and methods of classification can be found in Golay (1974) or in the proceedings of the Albany Colloquium edited by Philip and Hayes (1975).

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