Vegetative propagation of Ulmus villosa: effects of plant growth regulators, collection time, type of donor and position of shoot on adventitious root formation in stem cuttings

New Forests ◽  
2005 ◽  
Vol 29 (2) ◽  
pp. 105-116 ◽  
Author(s):  
D. R. Bhardwaj ◽  
V. K. Mishra
2004 ◽  
Vol 22 (3) ◽  
pp. 144-148 ◽  
Author(s):  
Lindsey Fox ◽  
Thayne Montague

Abstract Experiments investigated the influence of drought conditioning stock plants and an auxin-based plant growth regulator (PGR) on adventitious root formation of terminal stem cuttings taken from containerized firebush and Fraser's photinia stock plants. Drought conditioning (DC) treatments were initiated in a glass greenhouse and included: irrigation every day (no DC), irrigation every other day (medium DC), and irrigation every fourth day (high DC). Plants were subjected to DC for 16 consecutive days. Following DC treatments, all plants were irrigated and left overnight. Terminal stem cuttings were taken the following day and an 0.3% auxin talc formulation was used as the PGR. Plant growth regulator/DC treatments included: no DC ± PGR, medium DC ± PGR, and high DC ± PGR. Cuttings were placed under intermittent mist and heating pads maintained media temperature. After 21 and 78 days cuttings were evaluated for rooting percentage, number of roots, and length of the longest root. For firebush, rooting percentage was near 100% for all treatments. Number of roots and length of longest root for firebush cuttings was greatest for cuttings treated with PGR. For Fraser's photinia cuttings, rooting percentage was highest for cuttings treated with high DC + no PGR treatments. Cuttings treated with medium DC or high DC ± PGR treatment produced the greatest number of roots, while mean root length was longest for cuttings treated with medium DC + PGR and high DC + no PGR.


BMC Genomics ◽  
2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Carlos Villacorta-Martín ◽  
Ana Belén Sánchez-García ◽  
Joan Villanova ◽  
Antonio Cano ◽  
Miranda van de Rhee ◽  
...  

HortScience ◽  
2020 ◽  
Vol 55 (9) ◽  
pp. 1463-1467 ◽  
Author(s):  
Benjamin E. Deloso ◽  
Anders J. Lindström ◽  
Frank A. Camacho ◽  
Thomas E. Marler

The influences of indole-3-butyric acid (IBA) concentrations of 0–30 mg·g−1 on the success and speed of adventitious root development of Zamia furfuracea L.f. and Zamia integrifolia L.f. stem cuttings were determined. Root formation success for both species was greater than 95%. The IBA concentrations did not influence the speed of root development for Z. furfuracea, but the Z. integrifolia cuttings that received IBA concentration of 3 mg·g−1 generated adventitious roots more slowly than the cuttings in the control group. The ending dry weights of the stems, leaves, and roots were not influenced by IBA concentration for either species. Our results indicated that adventitious root formation on stem cuttings of these two Zamia species is successful without horticultural application of IBA. Additional IBA studies are needed on the other 300+ cycad species, especially those that are in a threatened category.


Author(s):  
Trân Huỳnh Ngọc Dương ◽  
Diễm Thị Lê ◽  
Mai Thị Bạch Võ

Abelmoschus sagittifolius Kurz is a medicinal plant with typical pharmacological of ginseng. However, the number of trees in the nature wild is declining rapidly due to the increasing demand for logging along with the narrowing of the distribution area and the low incidence of seed germination, affecting the use for researching and developing gene sources for drug production in many areas. In this plant, root is the most important organ of the plant, so the study of root formation in in vitro has been of great significance in assessing the effect of plant growth regulators on induction roots, as well as creating a source of starting material for studies on the biosynthesis of saponin in in vitro compounds as an alternative to outside planting. The results showed that after 2 weeks of culture, the germination rate was highest (88%) when the seeds were disinfected with HgCl2 0.1%, 3 minutes and then soaked in GA3 20,0 mg/L, 120 minutes, finally seed culture on MS + 20 g/L saccharose + GA3 5.0 mg/L + 7 g/L agar. The callus formation from hypocotyl in the environment on MS medium + 20 g/L sucrose + NAA 0.5 mg/L + BA 1.5 mg/L + 7 g/L agar was appropriate for root reduction and the best root formation was applied in the medium of MS + 20 g/L sucrose + IAA 0.3 mg/L + 7 g/L agar. In conclusion, the method of tissue culture is suitable for the formation of adventitious roots from callus formation from hypocotyl of Abelmoschus sagittifolius Kurz.


2020 ◽  
Vol 13 ◽  
pp. 194008292092059 ◽  
Author(s):  
Thomas E. Marler ◽  
Benjamin E. Deloso ◽  
Gil N. Cruz

Cycad stem cuttings will develop adventitious roots if the open wound is first treated with a sealant to protect the exposed parenchyma tissue. The commercial pruning wound sealant that is often employed for this purpose is not available in many locations. We used commonly available products as a prophylactic sealant on Cycas edentata , Cycas micronesica , and Cycas nitida cuttings to determine efficacy for sealing the wound and enabling adventitious root formation. Success was quantified after 7 months in a sand propagation substrate. Mortality was 100% for control cuttings with no wound sealant and about 60% for cuttings with candle wax as the sealant. Cuttings that received petroleum jelly, lanolin paste, modeling clay, honeycomb wax, or commercial pruning sealant exhibited 100% survival. Success in adventitious root formation ranged from 75% to 92% among the five successful prophylactic treatments and did not differ among the species. The results indicated that four of the products we evaluated were as effective as commercial pruning sealant for treating the exposed parenchyma on Cycas stem cuttings and enabling asexual propagation success. The candle wax was less effective because it was brittle and cracked to expose the stem’s parenchyma tissue.


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