Mating patterns in an indoor miniature Cryptomeria japonica seed orchard as revealed by microsatellite markers

New Forests ◽  
2009 ◽  
Vol 39 (3) ◽  
pp. 261-273 ◽  
Author(s):  
Yoshinari Moriguchi ◽  
Yoko Yamazaki ◽  
Hideaki Taira ◽  
Yoshihiko Tsumura
Keyword(s):  
Microsatellite Markers ◽  
Cryptomeria Japonica ◽  
Seed Orchard ◽  
Mating Patterns

Variation of paternal contribution in a seed orchard of Cryptomeria japonica determined using microsatellite markers

2004 ◽  
Vol 34 (8) ◽  
pp. 1683-1690 ◽  
Author(s):  
Yoshinari Moriguchi ◽  
Hideaki Taira ◽  
Naoki Tani ◽  
Yoshihiko Tsumura
Keyword(s):  
Microsatellite Markers ◽  
Cryptomeria Japonica ◽  
Pollen Dispersal ◽  
Seed Orchard ◽  
Null Alleles ◽  
Contamination Rate ◽  
Seed Crop ◽  
Pollen Contamination ◽  
Self Fertilization

We investigated gene flow and pollen contamination in a seed orchard of Cryptomeria japonica D. Don using six microsatellite markers. The quality of a seed crop is determined by many factors, including the rate of pollen contamination from outside sources, degree of self-fertilization, and equality of the clones as pollen donors. The surveyed seed orchard consisted of 62 clones and a total of 360 ramets. The seeds from 12 mother trees in the seed orchard were investigated using six highly polymorphic loci with high multipaternity exclusion probability (0.999). The estimated average pollen contamination rate was high at 47.78% (±4.12%), and the clonal self-fertilization rate was 2.22% (±0.94%) when we assumed that null alleles were present. The high pollen contamination rate could dramatically reduce the quality of the seed crop. The contamination rate differed from tree to tree, but the differences were not related to the location of each individual. The levels of contribution as pollen donors differed significantly amongst clones in the seed orchard. Differences in flowering phenology and relative pollen fecundity may be responsible for the differences in paternal contributions. The effective distance of pollen dispersal in this C. japonica seed orchard seemed to be greater than previously estimated.

scholarly journals Simple Genetic Distance-Optimized Field Deployments for Clonal Seed Orchards Based on Microsatellite Markers: As a Case of Chinese Pine Seed Orchard

PLoS ONE ◽  
2016 ◽  
Vol 11 (6) ◽  
pp. e0157646 ◽  
Author(s):  
Huwei Yuan ◽  
Shihui Niu ◽  
Yousry A. El-Kassaby ◽  
Yue Li ◽  
Wei Li
Keyword(s):  
Genetic Distance ◽  
Microsatellite Markers ◽  
Seed Orchard ◽  
Seed Orchards ◽  
Chinese Pine

Gene flow and mating system in five Cryptomeria japonica D. Don seed orchards as revealed by analysis of microsatellite markers

2005 ◽  
Vol 1 (4) ◽  
pp. 174-183 ◽  
Author(s):  
Yoshinari Moriguchi ◽  
Naoki Tani ◽  
Sinji Itoo ◽  
Fuminori Kanehira ◽  
Kouji Tanaka ◽  
...  
Keyword(s):  
Gene Flow ◽  
Microsatellite Markers ◽  
Mating System ◽  
Cryptomeria Japonica ◽  
Seed Orchards

Pollen contamination and mating patterns in a Douglas-fir seed orchard as measured by simple sequence repeat markers

2005 ◽  
Vol 35 (7) ◽  
pp. 1592-1603 ◽  
Author(s):  
Gancho T Slavov ◽  
Glenn T Howe ◽  
W Thomas Adams
Keyword(s):  
Ssr Markers ◽  
Simple Sequence Repeat ◽  
Female Parent ◽  
Seed Orchard ◽  
Douglas Fir ◽  
Sequence Repeat ◽  
Female Receptivity ◽  
Mating Patterns ◽  
Pollen Contamination ◽  
Simple Sequence

Pollen contamination is detrimental to the genetic quality of seed orchard crops. Highly variable simple sequence repeat (SSR) markers make it possible to accurately measure pollen contamination and characterize patterns of within-orchard mating by directly identifying the male and female parent of each seed produced in the orchard. We used nine SSR markers to measure pollen contamination and characterize mating patterns based on seed samples collected in 3 years (1999, 2000, and 2003) from one block of a nonisolated, open-pollinated, clonal seed orchard of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) in western Oregon. Pollen contamination was consistently high across the 3 years (mean = 35.3%, range = 31.0%-41.3%) and appeared to result primarily from cross-pollination among the orchard blocks. Levels of pollen contamination varied substantially among clones and were higher in clones with early female receptivity (mean = 55.5%) than in those with either mid (mean = 36.4%) or late (mean = 28.3%) female receptivity. We detected low rates of self-pollination (mean = 1.8% per clone) and over 10-fold differences in the relative paternal contributions of the clones. There was a clear pattern of positive assortative mating with respect to floral phenology. This study illustrates that SSR markers are powerful tools for characterizing seed lots and improving the design and management of Douglas-fir seed orchards.

scholarly journals Genetic variation, mating patterns and gene flow in a Pinus pinaster Aiton clonal seed orchard

2008 ◽  
Vol 65 (7) ◽  
pp. 706-706 ◽  
Author(s):  
Lúcia Fernandes ◽  
Margarida Rocheta ◽  
Jorge Cordeiro ◽  
Sandra Pereira ◽  
Sophie Gerber ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Gene Flow ◽  
Pinus Pinaster ◽  
Seed Orchard ◽  
Mating Patterns ◽  
Clonal Seed Orchard

scholarly journals Fertility Variation and Genetic Diversity in a Clonal Seed Orchard of Cryptomeria japonica

2005 ◽  
Vol 54 (1-6) ◽  
pp. 104-107 ◽  
Author(s):  
K. S. Kang ◽  
Y. A. El-Kassaby ◽  
M. S. Chung ◽  
C. S. Kim ◽  
Y. J. Kang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Cryptomeria Japonica ◽  
Seed Orchard ◽  
Clonal Seed Orchard ◽  
Fertility Variation ◽  
The Status ◽  
Status Number ◽  
Census Number ◽  
Male Strobilus ◽  
Male Strobili

Abstract Clonal differences in fertility (expressed as the number of female and male strobili) were determined for three consecutive years (2002-2004) in a clonal seed orchard of sugi (Cryptomeria japonica D. Don) in Korea. Fertility varied among clones and among years producing three-year averages of 196 and 652 for female and male strobili per ramet, respectively. Correlation between female and male strobilus production was positive over the three years and statistically significant in 2003, a good flowering year. Based on the observed fertility variation, the status numbers (Ns, measure of genetic diversity) were calculated and varied from 25.6 to 31.7 among the three studied years. On average (pooled), relative status number was 86% of the census number (N). Variation in female fertility was higher than that in male fertility, and this variation was reflected on female and male parents’ status numbers. Pooled Ns estimated from the three years was higher than that for any single year, implying that genetic diversity would increase when seeds collected from different years are pooled.

Mating success in a coastal Douglas-fir seed orchard as affected by distance and floral phenology

1989 ◽  
Vol 19 (10) ◽  
pp. 1248-1255 ◽  
Author(s):  
V. J. Erickson ◽  
W. T. Adams
Keyword(s):  
Genetic Markers ◽  
Mating Success ◽  
Strong Influence ◽  
Seed Orchard ◽  
Douglas Fir ◽  
Combined Effects ◽  
Regression Analyses ◽  
Mating Patterns ◽  
Floral Phenology ◽  
Cross Fertilization

The effect of distance and stage of phenological development on cross-pollination in a Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) seed orchard was investigated by estimating the proportion of viable embryos resulting from fertilization by designated male trees. The pollen source was identified by unique allozyme genetic markers that occurred in two clones in the orchard. In 38 mother trees, the proportion of embryos fertilized by the male marker ranged from 0 to 71.4%. Very little pollen was dispersed beyond 30 m. Within 30 m, mating success was only weakly associated with distance. Multiple regression analyses determined that phenological factors strongly influenced mating patterns; nearly one-third of the variation was dependent on a phenological variable (LMmf), which measured the combined effects of floral overlap and relative pollen fecundity of potential male parents. The interaction between LMmf and distance was highly significant, indicating that the floral status of mating pairs had a strong influence on the effect of distance. Our results suggest that a lack of complete floral synchrony between near neighbors may reduce preferential mating and thus help to promote cross-fertilization within Douglas-fir seed orchards.

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