cDNA Microarray Analysis of Nerve Growth Factor-Regulated Gene Expression Profile in Rat PC12 Cells

2005 ◽  
Vol 30 (4) ◽  
pp. 533-540 ◽  
Author(s):  
Kyung-Hee Lee ◽  
Chun Jeih Ryu ◽  
Hyo Jeong Hong ◽  
Jiyoung Kim ◽  
Eunjoo H. Lee
Keyword(s):  
Gene Expression ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Pc12 Cells ◽  
Gene Expression Profile ◽  
Microarray Analysis ◽  
Cdna Microarray ◽  
Expression Profile ◽  
Cdna Microarray Analysis ◽  
Regulated Gene Expression

scholarly journals cDNA Microarray Analysis of the Gene Expression Profile of Swine Muscle

2005 ◽  
Vol 18 (8) ◽  
pp. 1080-1087
Author(s):  
Chul Wook Kim ◽  
Kyu Tae Chang ◽  
Yeon Hee Hong ◽  
Won Yong Jung ◽  
Eun Jung Kwon ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Microarray Analysis ◽  
Cdna Microarray ◽  
Expression Profile ◽  
Cdna Microarray Analysis

Gene expression profile of endothelin-1-induced growth in glomerular mesangial cells

2003 ◽  
Vol 285 (5) ◽  
pp. C1109-C1115 ◽  
Author(s):  
Rangnath Mishra ◽  
Patrick Leahy ◽  
Michael S. Simonson
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Cell Growth ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Kinase Inhibitor ◽  
Mesangial Cells ◽  
Growth Control ◽  
Glomerular Mesangial Cells ◽  
Regulated Gene Expression

Endothelin (ET)-1 is a vasoconstrictor and mitogen involved in vascular remodeling. Changes in gene expression that underlie control of cell growth by ET-1 remain poorly characterized. To identify pathways of growth control we used microarrays to analyze ET-1-regulated gene expression in human mesangial cells, an important ET-1 vascular target cell in vivo. Statistical assessment of differential expression (significance analysis of microarrays) revealed upregulated transcripts for growth factors [heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF), fibroblast growth factor (FGF), interleukin (IL)-6] and downregulated transcripts for genes that inhibit growth (BAX, p27KIP1, DAD1). Consistent with the gene expression profile, quantitative RT-PCR and Western blotting confirmed induction of HB-EGF by ET-1. To test a functional role for HB-EGF in ET-1 signaling, we showed that exogenous HB-EGF stimulated phosphorylation of ErbB1 and growth of mesangial cells. ET-1-induced proliferation was blocked by an ErbB1 receptor-selective kinase inhibitor and by a specific ErbB1 receptor-neutralizing antibody. Proliferation in response to ET-1 was also inhibited by neutralizing antisera against human HB-EGF. Together, these results provide data for modeling ET-1 pathways for growth control and suggest a specific role for HB-EGF gene induction in mesangial cell growth in response to ET-1.

Differences in gene expression profile among SH-SY5Y neuroblastoma subclones with different neurite outgrowth responses to nerve growth factor

2005 ◽  
Vol 94 (5) ◽  
pp. 1264-1276 ◽  
Author(s):  
Tomoya Oe ◽  
Takeshi Sasayama ◽  
Takeyuki Nagashima ◽  
Masakazu Muramoto ◽  
Takao Yamazaki ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Neurite Outgrowth ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Nerve Growth
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