Functional characterization of Arabidopsis NaCl-inducible WRKY25 and WRKY33 transcription factors in abiotic stresses

In plants, heptahelical proteins (HHPs) have been shown to respond to a variety of abiotic stresses, including cold stress. Up to the present, the regulation mechanism of HHP5 under low temperature stress remains unclear. In this study, BcHHP5 was isolated from Pak-choi (Brassica rapa ssp. chinensis cv. Suzhouqing). Sequence analysis and phylogenetic analysis indicated that BcHHP5 in Pak-choi is similar to AtHHP5 in Arabidopsis thaliana. Structure analysis showed that the structure of the BcHHP5 protein is relatively stable and highly conservative. Subcellular localization indicated that BcHHP5 was localized on the cell membrane and nuclear membrane. Furthermore, real-time quantitative polymerase chain reaction (RT-qPCR) analysis showed that BcHHP5 was induced to express by cold and other abiotic stresses. In Pak-choi, BcHHP5-silenced assay, inhibiting the action of endogenous BcHHP5, indicated that BcHHP5-silenced might have a negative effect on cold tolerance, which was further confirmed. All of these results indicate that BcHHP5 might play a role in abiotic response. This work can serve as a reference for the functional analysis of other cold-related proteins from Pak-choi in the future.

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Functional characterization of two bZIP transcription factors in Verticillium dahliae

Gene ◽

10.1016/j.gene.2017.05.061 ◽

2017 ◽

Vol 626 ◽

pp. 386-394 ◽

Cited By ~ 6

Author(s):

Yulin Fang ◽

Dianguang Xiong ◽

Longyan Tian ◽

Chen Tang ◽

Yonglin Wang ◽

...

Keyword(s):

Transcription Factors ◽

Verticillium Dahliae ◽

Functional Characterization ◽

Bzip Transcription Factors

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Identification of wheat DREB genes and functional characterization of TaDREB3 in response to abiotic stresses

Gene ◽

10.1016/j.gene.2020.144514 ◽

2020 ◽

Vol 740 ◽

pp. 144514 ◽

Cited By ~ 2

Author(s):

Xin Niu ◽

Tengli Luo ◽

Hongyan Zhao ◽

Yali Su ◽

Wanquan Ji ◽

...

Keyword(s):

Abiotic Stresses ◽

Functional Characterization

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Characterization of novel regulators for heat stress tolerance in tomato from Indian sub-continent

10.1101/800607 ◽

2019 ◽

Author(s):

Sonia Balyan ◽

Sombir Rao ◽

Sarita Jha ◽

Chandni Bansal ◽

Jaishri Rubina Das ◽

...

Keyword(s):

Transcription Factors ◽

Heat Stress ◽

Functional Characterization ◽

Transcriptome Profiling ◽

Negative Regulator ◽

Comparative Transcriptome ◽

Regulatory Pathways ◽

Cool Region ◽

Promoter Swapping

AbstractThe footprint of tomato cultivation, a cool region crop that exhibits heat stress (HS) sensitivity, is increasing in the tropics/sub-tropics. Knowledge of novel regulatory hot-spots from varieties growing in the Indian sub-continent climatic zones could be vital for developing HS-resilient crops. Comparative transcriptome-wide signatures of a tolerant (CLN1621L) and sensitive (CA4) cultivar-pair short-listed from a pool of varieties exhibiting variable thermo-sensitivity using physiological, survival and yield-related traits revealed redundant to cultivar-specific HS-regulation with more up-regulated genes for CLN1621L than CA4. The anatgonisiticly-expressing genes include enzymes; have roles in plant defense and response to different abiotic stresses. Functional characterization of three antagonistic genes by overexpression and TRV-VIGS silencing established Solyc09g014280 (Acylsugar acyltransferase) and Solyc07g056570 (Notabilis), that are up-regulated in tolerant cultivar, as positive regulators of HS-tolerance and Solyc03g020030 (Pin-II proteinase inhibitor), that is down-regulated in CLN1621L, as negative regulator of thermotolerance. Transcriptional assessment of promoters of these genes by SNPs in stress-responsive cis-elements and promoter swapping experiments in opposite cultivar background showed inherent cultivar-specific orchestration of transcription factors in regulating transcription. Moreover, overexpression of three ethylene response transcription factors (ERF.C1/F4/F5) also improved HS-tolerance in tomato. This study identifies several novel HS-tolerance genes and provides proof of their utility in tomato-thermotolerance.HighlightNovel heat stress regulatory pathways uncovered by comparative transcriptome profiling between contrasting tomato cultivars from Indian sub-continent for improving thermotolerance. (20/30)

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Genome-wide analysis of NDR1/HIN1-like genes in pepper (Capsicum annuum L.) and functional characterization of CaNHL4 under biotic and abiotic stresses

Horticulture Research ◽

10.1038/s41438-020-0318-0 ◽

2020 ◽

Vol 7 (1) ◽

Cited By ~ 1

Author(s):

Changyun Liu ◽

Haoran Peng ◽

Xinyu Li ◽

Chaolong Liu ◽

Xing Lv ◽

...

Keyword(s):

Capsicum Annuum ◽

Abiotic Stresses ◽

Functional Characterization ◽

Biotic And Abiotic Stresses ◽

Genome Wide Analysis ◽

Capsicum Annuum L ◽

Genome Wide

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Transcription factors–DNA interactions in rice: identification and verification

Briefings in Bioinformatics ◽

10.1093/bib/bbz045 ◽

2019 ◽

Vol 21 (3) ◽

pp. 946-956 ◽

Cited By ~ 5

Author(s):

Zijie Shen ◽

Yuan Lin ◽

Quan Zou

Keyword(s):

Transcription Factors ◽

Functional Characterization ◽

Rice Genome ◽

Research Strategies ◽

Promoter Regions ◽

Rice Varieties ◽

Rice Genome Sequence ◽

Advantages And Disadvantages ◽

Genomics Research

Abstract The completion of the rice genome sequence paved the way for rice functional genomics research. Additionally, the functional characterization of transcription factors is currently a popular and crucial objective among researchers. Transcription factors are one of the groups of proteins that bind to either enhancer or promoter regions of genes to regulate expression. On the basis of several typical examples of transcription factor analyses, we herein summarize selected research strategies and methods and introduce their advantages and disadvantages. This review may provide some theoretical and technical guidelines for future investigations of transcription factors, which may be helpful to develop new rice varieties with ideal traits.

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