Specific region affects the difference in accumulation levels between apple food allergen Mal d 1 and birch pollen allergen Bet v 1 which are expressed in vegetative tissues of transgenic rice

2018 ◽  
Vol 98 (4-5) ◽  
pp. 439-454
Author(s):  
Fumio Takaiwa ◽  
Yuko Ogo ◽  
Yuhya Wakasa
Keyword(s):  
Transgenic Rice ◽  
Food Allergen ◽  
Birch Pollen ◽  
Specific Region ◽  
Pollen Allergen ◽  
Vegetative Tissues ◽  
Bet V 1 ◽  
Birch Pollen Allergen ◽  
Mal D 1 ◽  
The Difference

scholarly journals Difference in accumulation pattern of the allergens within the same PR10 family in transgenic rice

2018 ◽  
Author(s):  
Fumio Takaiwa ◽  
Yuko Ogo ◽  
Yuhya Wakasa
Keyword(s):  
Transgenic Rice ◽  
Birch Pollen ◽  
Secretory Protein ◽  
Aleurone Layer ◽  
Accumulation Pattern ◽  
Vegetative Tissues ◽  
Bet V 1 ◽  
Birch Pollen Allergen ◽  
Mal D 1 ◽  
Post Transcriptional Regulation

AbstractApple food allergen Mal d 1 and birch pollen allergen Bet v 1 belong to the same pathogen related protein 10 (PR10) family. When each of these allergens was expressed as a secretory protein by fusion with the GFP reporter in transgenic rice by ligating an N terminal signal peptide and a C terminal KDEL ER retention signal under the control of the maize ubiquitin constitutive promoter, the GFP:Mald1 highly accumulated in various tissues, whereas the accumulation level of GFP:Betv1 was remarkably reduced in vegetative tissues except for seed. Analysis by RT-PCR exhibited that there was little difference in transcript levels between them, indicating the involvement of post-transcriptional regulation. To investigate the cause of such difference in accumulation levels, deletion analysis of the Mal d 1 and domain swapping between them were carried out in transgenic rice. These results showed that the region between positions 41-90 in the Mal d 1 is predominantly implicated in higher level accumulation in vegetative tissues as well as seed compared with the Bet v 1. It is notable that GFP:Mald1 directed by the ubiquitin promoter is deposited in huge PBs in aleurone layer rather than starchy endosperm.HighlightSpecific region of PR10 proteins is mainly implicated in their stability in vegetative tissues when expressed in transgenic rice.

scholarly journals Transgenic Rice Seeds Accumulating Recombinant Hypoallergenic Birch Pollen Allergen Bet v 1 Generate Giant Protein Bodies

2013 ◽  
Vol 54 (6) ◽  
pp. 917-933 ◽  
Author(s):  
Shuyi Wang ◽  
Hideyuki Takahashi ◽  
Hiroyuki Kajiura ◽  
Taiji Kawakatsu ◽  
Kazuhito Fujiyama ◽  
...  
Keyword(s):  
Transgenic Rice ◽  
Birch Pollen ◽  
Pollen Allergen ◽  
Protein Bodies ◽  
Bet V 1 ◽  
Birch Pollen Allergen ◽  
Rice Seeds

scholarly journals Caveolar transport through nasal epithelium of birch pollen allergen Bet v 1 in allergic patients

2009 ◽  
Vol 124 (1) ◽  
pp. 135-142.e21 ◽  
Author(s):  
Sakari Joenväärä ◽  
Pirkko Mattila ◽  
Jutta Renkonen ◽  
Antti Mäkitie ◽  
Sanna Toppila-Salmi ◽  
...  
Keyword(s):  
Birch Pollen ◽  
Pollen Allergen ◽  
Nasal Epithelium ◽  
Bet V 1 ◽  
Birch Pollen Allergen

scholarly journals Genetic Variants in CHIA and CHI3L1 Are Associated with the IgE Response to the Ascaris Resistance Marker ABA-1 and the Birch Pollen Allergen Bet v 1

PLoS ONE ◽  
2016 ◽  
Vol 11 (12) ◽  
pp. e0167453 ◽  
Author(s):  
Nathalie Acevedo ◽  
Adriana Bornacelly ◽  
Dilia Mercado ◽  
Per Unneberg ◽  
Irene Mittermann ◽  
...  
Keyword(s):  
Genetic Variants ◽  
Birch Pollen ◽  
Pollen Allergen ◽  
Resistance Marker ◽  
Bet V 1 ◽  
Birch Pollen Allergen ◽  
Ige Response

Two members of the Bet v 1 gene family encoding birch pathogenesis-related proteins display different patterns of root expression and wound-inducibility

1998 ◽  
Vol 25 (4) ◽  
pp. 459 ◽  
Author(s):  
Pascal Poupard ◽  
Désiré-Georges Strullu ◽  
Philippe Simoneau
Keyword(s):  
Birch Pollen ◽  
Polyclonal Antiserum ◽  
Pollen Allergen ◽  
Pathogenesis Related Proteins ◽  
Bet V 1 ◽  
New Family ◽  
Pathogenesis Related ◽  
Birch Pollen Allergen ◽  
Root Organogenesis ◽  
Related Proteins

Bet v 1-SC genes constitute a subset of a multigene family in birch (Betula pendula Roth) that includes the gene for the major pollen allergen and encode proteins belonging to a new family of intracellular pathogenesis-related proteins. We have investigated the expression in birch roots of the Bet v 1-SC1 and Bet v 1-SC3 genes by northern and reverse-transcription-polymerase chain reaction analyses. Only Bet v 1-SC3 was found to be highly expressed in roots under basal conditions but this gene was not wound-inducible. Conversely the expression of the Bet v 1-SC1 gene was markedly transiently induced 8 to 9 h after the stress. Immunoblotting with polyclonal antiserum raised against the major birch pollen allergen confirmed that Bet v 1-related polypeptides are present in root extracts. This first report of Bet v 1-SC gene expression and wound-inducibility in birch roots suggests a possible function of proteins encoded by these genes in plant defense and/or root organogenesis.

Mimotopes of birch pollen allergen Bet v 1 conjugated to albumin inhibit antibody binding to the natural antigen

2002 ◽  
Vol 109 (1) ◽  
pp. S130-S130
Author(s):  
Angelika Riemer ◽  
Isabella Schoell ◽  
Farideh Nasseri-Kia ◽  
Claudia Gusenbauer ◽  
Margarete Focke ◽  
...  
Keyword(s):  
Birch Pollen ◽  
Antibody Binding ◽  
Pollen Allergen ◽  
Bet V 1 ◽  
Birch Pollen Allergen ◽  
Inhibit Antibody
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