Cloning and characterization of a differentially expressed cDNA encoding myo-inositol-1-phosphate synthase involved in response to abiotic stress in Jatropha curcas

2011 ◽  
Vol 106 (2) ◽  
pp. 269-277 ◽  
Author(s):  
Yong Wang ◽  
Jing Huang ◽  
Chun Bao Gou ◽  
Xiao Dai ◽  
Fang Chen ◽  
...  
Author(s):  
M.Z. Piña ◽  
C. Castanedo ◽  
E. Escamilla ◽  
E. Piña

Planta ◽  
2018 ◽  
Vol 248 (5) ◽  
pp. 1121-1141 ◽  
Author(s):  
Papri Basak ◽  
Shiny Sangma ◽  
Abhishek Mukherjee ◽  
Tanushree Agarwal ◽  
Sonali Sengupta ◽  
...  

1984 ◽  
Vol 4 (11) ◽  
pp. 2479-2485 ◽  
Author(s):  
B S Loewy ◽  
S A Henry

We isolated a mutant of Saccharomyces cerevisiae defective in the formation of phosphatidylcholine via methylation of phosphatidylethanolamine. The mutant synthesized phosphatidylcholine at a reduced rate and accumulated increased amounts of methylated phospholipid intermediates. It was also found to be auxotrophic for inositol and allelic to an existing series of ino4 mutants. The ino2 and ino4 mutants, originally isolated on the basis of an inositol requirement, are unable to derepress the cytoplasmic enzyme inositol-1-phosphate synthase (myo-inositol-1-phosphate synthase; EC 5.5.1.4). The INO4 and INO2 genes were, thus, previously identified as regulatory genes whose wild-type product is required for expression of the INO1 gene product inositol-1-phosphate synthase (T. Donahue and S. Henry, J. Biol. Chem. 256:7077-7085, 1981). In addition to the identification of a new ino4-allele, further characterization of the existing series of ino4 and ino2 mutants, reported here, demonstrated that they all have a reduced capacity to convert phosphatidylethanolamine to phosphatidylcholine. The pleiotropic phenotype of the ino2 and ino4 mutants described in this paper suggests that the INO2 and INO4 loci are involved in the regulation of phospholipid methylation in the membrane as well as inositol biosynthesis in the cytoplasm.


2019 ◽  
Vol 13 (3) ◽  
pp. 235-243 ◽  
Author(s):  
Wenbin Wang ◽  
Huan Yu ◽  
Ho Soo Kim ◽  
Yanxin Yang ◽  
Xiangpo Qiu ◽  
...  

1982 ◽  
Vol 186 (2) ◽  
pp. 157-163 ◽  
Author(s):  
Miriam L. Greenberg ◽  
Paul Goldwasser ◽  
Susan A. Henry

2017 ◽  
Author(s):  
Tyson C. C. Kerr ◽  
Haggag Abdel-Mageed ◽  
MiYoung Kang ◽  
Dakota Cryer ◽  
Randy D. Allen

AbstractThe AREB/ABF bZIP transcription factors play a pivotal role in abscisic acid-dependent abiotic stress-responsive gene expression. Despite the perennial damage and reduced productivity that result from water-deficit and unpredictable early season temperature fluctuations, these critical genes have not been previously examined in upland cotton (Gossypium hirsutum). Here, we report the isolation of the G. hirsutum ABF homologs, characterization of their expression patterns in response to abiotic stress treatments, and examination of their functions through heterologous ectopic expression in Arabidopsis. As expected for an allotetraploid, G. hirsutum ABF homologs are present in the genome as homeologous pairs. These genes are differentially expressed, both among the homologs and within the homeologous pairs, in response to exogenous abscisic acid (ABA) application, dehydration, and chilling temperatures. Furthermore, heterologous ectopic expression of many of the G. hirsutum ABF genes in Arabidopsis conferred increased tolerance to water deficit and osmotic stress, as well as cold tolerance, in a gene specific manner. These results indicate the G. hirsutum ABF homologs are functional in Arabidopsis and, as in other species, are likely to play an essential role in the abiotic stress response.HighlightThe Gossypium hirsutum ABF homeologs are differentially expressed in response to abiotic stress, and their ectopic expression in Arabidopsis can confer increased water deficit tolerance.


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