Effect of plant growth regulators on the accumulation of indolizidine alkaloids in Securinega suffruticosa callus cultures

Pueraria candollei Wall. ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66 - 100% of leaf or stem explants formed calli. Calli from stem explants cultured on MS medium supplemented with 0.5 mg/l thidiazuron (TDZ) gave the maximum of shoot induction (16%) and the highest level of total isoflavonoids [(50.39 ± 7.06) mg/g dry wt], which was 7-fold higher than that of the native tuber [(7.04 ± 0.29) mg/g dry wt]. These results suggest that addition of TDZ to the culture medium markedly enhances the production of isoflavonoids in calli induced from stem explants of P. candollei var. mirifica.

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Compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulators

Plant Science ◽

10.1016/j.plantsci.2011.10.008 ◽

2012 ◽

Vol 185-186 ◽

pp. 169-175 ◽

Cited By ~ 10

Author(s):

Paula Alayón-Luaces ◽

Nora M.A. Ponce ◽

Luis A. Mroginski ◽

Carlos A. Stortz ◽

Gabriel O. Sozzi

Keyword(s):

Cell Wall ◽

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Callus Cultures ◽

Apple Fruit ◽

Cell Wall Polysaccharides ◽

Compositional Changes

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Sterilization protocols and the effect of plant growth regulators on callus induction and secondary metabolites production in in vitro cultures Melia azedarach L.

AMB Express ◽

10.1186/s13568-022-01343-8 ◽

2022 ◽

Vol 12 (1) ◽

Author(s):

Fatemeh Ahmadpoor ◽

Nasser Zare ◽

Rasool Asghari ◽

Parisa Sheikhzadeh

Keyword(s):

Secondary Metabolites ◽

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Callus Induction ◽

Callus Cultures ◽

Melia Azedarach ◽

Ms Medium ◽

Fresh Weight ◽

Metabolites Production

AbstractMelia azedarach L. is a valuable source of antioxidants and secondary metabolites. This study is a first extensive report about the effect of different serialization protocols and plant growth regulators (PGRs) on explant disinfection efficiency, callus induction and secondary metabolites production and accumulation in callus cultures of M. azedarach L. In this regard, the effect of plant growth regulators on callus induction and secondary metabolites production were examined. In addition, different sterilization agents were evaluated for disinfection of chinaberry leaf explants. The results showed that the lowest percentage of explant contamination and browning with the highest percentage of callus induction and callus growth obtained with explants pretreated with benomyl (2 g/L) for 2 h and sterilized with 7% H2O2 for 10 min and NaOCl 2% (without pH adjustment) for 12 min. Although adjusting the pH of NaOCl to pH = 7 and 10 significantly reduced the microbial contamination and increased the percentage of contamination-free cultures of M. azedarach L., adversely influenced the explant viability and callus induction and growth. The highest percentage of callus induction obtained on the MS medium containing 3 mg/L NAA/2,4-D and 1 or 3 mg/L Kin/BAP, and the highest callus yield (1804.833 mg/explant) belonged to the MS medium supplemented with 5 mg/L 2,4-D and 5 mg/L Kin. The callus cultures grown on the MS medium supplemented with 3 mg/L NAA and 1 mg/L Kin produced the highest amount of Quercetin (2.06 mg/g fresh weight), Rutin (5.56 mg/g fresh weight) and Kaempferol (1.84 mg/g fresh weight).

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Optimization of Biomass Accumulation and Production of Phenolic Compounds in Callus Cultures of Rhodiola rosea L. Using Design of Experiments

Plants ◽

10.3390/plants11010124 ◽

2022 ◽

Vol 11 (1) ◽

pp. 124

Author(s):

Anna A. Erst ◽

Anastasia A. Petruk ◽

Andrey S. Erst ◽

Denis A. Krivenko ◽

Nadezhda V. Filinova ◽

...

Keyword(s):

Phenolic Compounds ◽

Plant Growth ◽

Design Of Experiments ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Biomass Accumulation ◽

Callus Cultures ◽

Rhodiola Rosea ◽

Total Phenolic

Rhodiola rosea L. is a valuable medicinal plant with adaptogenic, neuroprotective, antitumor, cardioprotective, and antidepressant effects. In this study, design of experiments methodology was employed to analyze and optimize the interacting effects of mineral compounds (concentration of NO3− and the ratio of NH4+ to K+) and two plant growth regulators [total 6-benzylaminopurine (BAP) and α-naphthylacetic acid (NAA) concentration and the ratio of BAP to NAA] on the growth and the production of total phenolic compounds (TPCs) in R. rosea calluses. The overall effect of the model was highly significant (p < 0.0001), indicating that NH4+, K+, NO3−, BAP, and NAA significantly affected growth. The best callus growth (703%) and the highest production of TPCs (75.17 mg/g) were achieved at an NH4+/K+ ratio of 0.33 and BAP/NAA of 0.33, provided that the concentration of plant growth regulators was 30 μM and that of NO3− was ≤40 mM. According to high-performance liquid chromatography analyses of aerial parts (leaves and stems), in vitro seedlings and callus cultures of R. rosea contain no detectable rosarin, rosavin, rosin, and cinnamyl alcohol. This is the first report on the creation of an experiment for the significant improvement of biomass accumulation and TPC production in callus cultures of R. rosea.

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