Chromosome number, genome size, seed storage protein profile and competence for direct somatic embryo formation in Algerian annual Medicago species

Bambara groundnut (Vigna subterranea) is a leguminous crop that is considered underutilized and has previously received little research attention. Variability in a number of physiological factors, including germination rate, widely affects its production. Seed storage protein, its fractions and protein profile of six Bambara groundnut local landraces were studied to assess their genetic relatedness. Total seed storage protein and its fractions were estimated by Bradford’s method. SDS-PAGE analysis was used to evaluate storage protein profile. The results showed significant differences (p<0.05) in protein contents among the landraces. The major seed storage proteins were found to be globulins (0.048 to 0.088mg/mL ), albumins (0.023 to 0.038mg/mL ), glutelins (0.007 to 0.013mg/mL ) and prolamins (0.002 to 0.004mg/mL ). Five peptide bands were detected with molecular weights corresponding to 97.4 kDa, 45 kDa, 29 kDa, 20.1 kDa and 18 kDa, respectively. Three peptide bands corresponding to 97.4 kDa, 45 kDa and 18 kDa were detected in all the landraces and two peptide bands between 29 kDa and 20.1 kDa were detected in five landraces. Dendrogram generated by UPGMA grouped the six landraces into one major cluster with two sub-clusters. The observed diversity in storage protein pattern of the landraces indicated their potential as materials for crop improvement.

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Seed storage protein changes and mobilization pattern in Bambaranut (Vigna subterranea) (L.) Verdc. during germination

Plant Science Today ◽

10.14719/pst.2021.8.4.1257 ◽

2021 ◽

Vol 8 (4) ◽

Author(s):

Najib M Saminu ◽

Yusuf Y Muhammad

Keyword(s):

Amino Acids ◽

Gibberellic Acid ◽

Storage Protein ◽

Free Amino Acids ◽

Free Amino ◽

Storage Proteins ◽

Seed Storage Protein ◽

Protein Profile ◽

Seed Storage ◽

Germination Period

The germination of seeds involves series of events during which mobilization and utilization of seed storage proteins occur. This study is aimed at determining the changes in total and fractions of seed storage protein in six bambaranut landraces during 96 hrs germination period. The study assessed the changes in seed storage protein content, storage protein profile, endopeptidase activity, free amino acids and gibberellic acid levels. Significant (p<0.05) decrease in total storage protein after 24 hrs, albumin from 48 hrs and globulin and glutelin after 72 hrs germination period were observed in the studied landraces. Prolamin showed significant (p<0.05) decrease after 48 hrs in all the landraces. Five peptide bands were detected in the six landraces with molecular weights corresponding to 97.4 kDa, 45 kDa, 29 kDa, 20.1 kDa and 18 kDa. Peptide bands with molecular weight of 97 kDa and 29 kDa decreased in intensity after 48 hrs of germination in four landraces. Free amino acids content significantly (p<0.05) increased following 24 hrs germination period in all the landraces. The activity of endopeptidase increased significantly (p<0.05), reaching maximum after 96 hrs germination. Significant (p<0.05) increase in gibberellic acid level throughout germination period was also observed. Although slow degradation rate of storage proteins was observed, there was variation in the rate at which storage protein and its fractions decreased among the bambaranut landraces during germination. This variation could be utilized towards obtaining improved bambaranut genotypes with better germination characteristics.

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Seed storage protein variation in Arachis species

Genome ◽

10.1139/g94-068 ◽

1994 ◽

Vol 37 (3) ◽

pp. 487-496 ◽

Cited By ~ 16

Author(s):

P. G. Lanham ◽

B. P. Forster ◽

P. McNicol ◽

J. P. Moss ◽

W. Powell

Keyword(s):

Storage Protein ◽

Seed Protein ◽

Storage Proteins ◽

Seed Storage Protein ◽

Protein Profile ◽

Seed Storage ◽

Protein Polymorphism ◽

Protein Patterns ◽

Protein Variation ◽

Arachis Duranensis

Seventy-two accessions, representing 22 species from sections Arachis, Erectoides, Extranervosae, and Triseminalae of the genus Arachis, were screened for seed storage protein polymorphism. Variation was detected between sections, between genome types, between species, and in some cases between different accessions of the same species or different seeds of the same accession. Arachis duranensis and one accession of A. cardenasii were found to have identical protein patterns. The greatest dissimilarity was found between species of the section Extranervosae and species of the section Triseminalae. Those of section Erectoides showed much similarity with some species of section Arachis. Protein polymorphism was shown to distinguish the two subspecies of A. hypogaea (fastigiata and hypogaea) in 27 of 28 cases. The seed protein profile of A. monticola was a combination of seed protein profiles from the two A. hypogaea subspecies. The relatedness between the various species was calculated and those that had the greatest similarity with A. hypogaea were A. spegazzinii and A. batizocoi.Key words: Arachis, groundnut, storage proteins, variation.

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Identification of seed storage protein markers for drought tolerance in mungbean

Research in Biotechnology ◽

10.19071/rib.2016.v7.2895 ◽

2016 ◽

Vol 7 (2) ◽

Cited By ~ 1

Author(s):

Swapan Kumar Tripathy ◽

Priyadarshini Mohanty ◽

Monalisha Jena ◽

Sasmita Dash ◽

Devraj Lenka ◽

...

Keyword(s):

Drought Tolerance ◽

Storage Protein ◽

Vigna Radiata ◽

Seed Storage Protein ◽

Protein Profile ◽

Seed Storage ◽

Protein Profiling ◽

Wild Accession ◽

Drought Tolerant ◽

Polypeptide Band

A set of 292 mungbean germplasm accessions including 62 popularly adapted local land races and two wild forms (Vigna radiata var. sublobata), important breeding lines and standard ruling varieties were screened for drought stress tolerance at seedling stage. Eight genotypes e.g., C. No. 35, OUM 14-1, OUM 49-2, Pusa 9072, OM 99-3, Banapur local B, Nipania munga, Kalamunga 1-A) have been identified to possess drought tolerance. Globulin seed storage protein profiling was carried out in 19 selected mungbean genotypes comprising eight drought tolerant, seven drought sensitive, two wild forms of mungbean (TCR 20 and TCR 213) and two standard checks (LGG 460 and T 2-1) to explore differentially expressed polypeptides. Seed protein profiles revealed 15 scorable polypeptide bands with molecular weights ranging from 10.0 to 102.2kD. A specific 12.8kD polypeptide band was present in all above drought tolerant test genotypes including the wild accession TCR 20. Such a polypeptide band may serve as useful biochemical marker for identification of drought tolerant genotypes in mungbean. Key words: Genetic diversity, seed storage protein profile, wild and cultivated Vigna radiata.

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Molecular cytogenetical and biochemical studies on some Lupinus species

Bulletin of the National Research Centre ◽

10.1186/s42269-021-00493-2 ◽

2021 ◽

Vol 45 (1) ◽

Author(s):

Hoda B. M. Ali ◽

Samy A. A. Heiba

Keyword(s):

Related Species ◽

Storage Protein ◽

Seed Storage Protein ◽

Lupinus Albus ◽

Seed Storage ◽

Rrna Genes ◽

Large Chromosome ◽

45S Rdna ◽

Lupinus Polyphyllus ◽

Electrophoretic Patterns

Abstract Background Lupins are cultivated as human consumption grains and forage legumes. The chromosomes of lupins are too small to be karyotyped by conventional techniques, because they reveal a general lack of distinctive cytological features. In the current study, Fluorescence in situ Hybridization (FISH) was used to locate 5S and 45S ribosomal gene sites on the chromosomes of Lupinus albus ssp albus, Lupinus albus ssp graecus, Lupnus termis (all with 2n = 50), and Lupinus polyphyllus lindl var. polyphyllus (2n = 48), FISH together with seed storage protein electrophoretic patterns were used to find out the relationship among these species. Results The double-target FISH on the chromosomes of the studied species with rDNA probes revealed that the two types of rRNA genes are located on different chromosomes. The detected loci of rRNA genes partially reflected the taxonomical similarity among the two Lupinus albus subspecies and L. termis. Lupinus polyphyllus lindl var. polyphyllus was exception by having unique large chromosome mostly is covered by one signal of 45S rDNA, whereas its homologous chromosome seems to be normal-sized and have the other 45S rDNA locus. The similarity matrix among the Lupinus species as computed according to Jaccardʼs Coefficient from the SDS-PAGE, showed that L. albus ssp. Albus and L. albus ssp. Graecus are the most similar species (~ 97%), and then comes L. termis, and L. polyphyllus lindl var. polyphylus has been placed in separate clade and still the most related species to it among the studied species is L. termis (~ 70%). Conclusion It could be postulated from FISH and seed storage protein electrophoretic patterns that the relationships among the studied species is as follows, Lupinus albus ssp albus, is the most related species to Lupinus albus ssp graecus then comes Lupnus termis and Lupinus polyphyllus lindl var. polyphyllus at a distal position.

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Ana o 1, a cashew (Anacardium occidental) allergen of the vicilin seed storage protein family

Journal of Allergy and Clinical Immunology ◽

10.1067/mai.2002.125208 ◽

2002 ◽

Vol 110 (1) ◽

pp. 160-166 ◽

Cited By ~ 106

Author(s):

Fang Wang ◽

Jason M. Robotham ◽

Suzanne S. Teuber ◽

Pallavi Tawde ◽

Shridhar K. Sathe ◽

...

Keyword(s):

Storage Protein ◽

Seed Storage Protein ◽

Seed Storage ◽

Protein Family

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Highly Recombinogenic Regions at Seed Storage Protein Loci on Chromosome 1DS of Aegilops tauschii, the D-Genome Donor of Wheat

Genetics ◽

10.1093/genetics/155.1.361 ◽

2000 ◽

Vol 155 (1) ◽

pp. 361-367 ◽

Cited By ~ 3

Author(s):

Wolfgang Spielmeyer ◽

Odile Moullet ◽

André Laroche ◽

Evans S Lagudah

Keyword(s):

Genetic Distance ◽

Storage Protein ◽

Seed Storage Protein ◽

Aegilops Tauschii ◽

Bac Library ◽

Seed Storage ◽

D Genome ◽

Rflp Map ◽

Genome Donor ◽

The Relationship

Abstract A detailed RFLP map was constructed of the distal end of the short arm of chromosome 1D of Aegilops tauschii, the diploid D-genome donor species of hexaploid wheat. Ae. tauschii was used to overcome some of the limitations commonly associated with molecular studies of wheat such as low levels of DNA polymorphism. Detection of multiple loci by most RFLP probes suggests that gene duplication events have occurred throughout this chromosomal region. Large DNA fragments isolated from a BAC library of Ae. tauschii were used to determine the relationship between physical and genetic distance at seed storage protein loci located at the distal end of chromosome 1DS. Highly recombinogenic regions were identified where the ratio of physical to genetic distance was estimated to be <20 kb/cM. These results are discussed in relation to the genome-wide estimate of the relationship between physical and genetic distance.

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