Isolation and functional characterization of a R2R3-MYB regulator of Prunus mume anthocyanin biosynthetic pathway

2017 ◽  
Vol 131 (3) ◽  
pp. 417-429 ◽  
Author(s):  
Qin Zhang ◽  
Ruijie Hao ◽  
Zongda Xu ◽  
Weiru Yang ◽  
Jia Wang ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
Functional Characterization ◽  
Prunus Mume ◽  
Anthocyanin Biosynthetic Pathway ◽  
R2r3 Myb

Isolation and functional characterization of a R2R3-MYB regulator of the anthocyanin biosynthetic pathway from Epimedium sagittatum

2016 ◽  
Vol 35 (4) ◽  
pp. 883-894 ◽  
Author(s):  
Wenjun Huang ◽  
A. B. M. Khaldun ◽  
Haiyan Lv ◽  
Liuwen Du ◽  
Chanjuan Zhang ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
Functional Characterization ◽  
Anthocyanin Biosynthetic Pathway ◽  
Epimedium Sagittatum ◽  
R2r3 Myb

scholarly journals The MIR-Domain of PbbHLH2 Is Involved in Regulation of the Anthocyanin Biosynthetic Pathway in ”Red Zaosu” (PyrusBretschneideri Rehd.) Pear Fruit

2021 ◽  
Vol 22 (6) ◽  
pp. 3026
Author(s):  
Xieyu Li ◽  
Fangxin Xiang ◽  
Wei Han ◽  
Bingqing Qie ◽  
Rui Zhai ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
Anthocyanin Biosynthesis ◽  
Bimolecular Fluorescence Complementation ◽  
Anthocyanin Content ◽  
Pear Fruit ◽  
Fruit Peel ◽  
Interaction Domain ◽  
Dihydroflavonol Reductase ◽  
Anthocyanin Biosynthetic Pathway ◽  
R2r3 Myb

The N-terminal of Myc-like basic helix-loop-helix transcription factors (bHLH TFs) contains an interaction domain, namely the MYB-interacting region (MIR), which interacts with the R2R3-MYB proteins to regulate genes involved in the anthocyanin biosynthetic pathway. However, the functions of MIR-domain bHLHs in this pathway are not fully understood. In this study, PbbHLH2 containing the MIR-domain was identified and its function investigated. The overexpression of PbbHLH2 in ”Zaosu” pear peel increased the anthocyanin content and the expression levels of late biosynthetic genes. Bimolecular fluorescence complementation showed that PbbHLH2 interacted with R2R3-MYB TFs PbMYB9, 10, and 10b in onion epidermal cells and confirmed that MIR-domain plays important roles in the interaction between the MIR-domain bHLH and R2R3-MYB TFs. Moreover, PbbHLH2 bound and activated the dihydroflavonol reductase promoter in yeast one-hybrid (Y1H) and dual-luciferase assays. Taken together these results suggested that the MIR domain of PbbHLH2 regulated anthocyanin biosynthesis in pear fruit peel.

scholarly journals Functional characterization of the switchgrass (Panicum virgatum) R2R3-MYB transcription factor PvMYB4 for improvement of lignocellulosic feedstocks

2011 ◽  
Vol 193 (1) ◽  
pp. 121-136 ◽  
Author(s):  
Hui Shen ◽  
Xianzhi He ◽  
Charleson R. Poovaiah ◽  
Wegi A. Wuddineh ◽  
Junying Ma ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Panicum Virgatum ◽  
Functional Characterization ◽  
Myb Transcription Factor ◽  
Lignocellulosic Feedstocks ◽  
R2r3 Myb

scholarly journals Cloning and Characterization of a Putative R2R3 MYB Transcriptional Repressor of the Rosmarinic Acid Biosynthetic Pathway from Salvia miltiorrhiza

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e73259 ◽  
Author(s):  
Shuncang Zhang ◽  
Pengda Ma ◽  
Dongfeng Yang ◽  
Wenjing Li ◽  
Zongsuo Liang ◽  
...  
Keyword(s):  
Rosmarinic Acid ◽  
Biosynthetic Pathway ◽  
Salvia Miltiorrhiza ◽  
Transcriptional Repressor ◽  
R2r3 Myb

scholarly journals An R2R3 MYB transcription factor associated with regulation of the anthocyanin biosynthetic pathway in Rosaceae

2010 ◽  
Vol 10 (1) ◽  
pp. 50 ◽  
Author(s):  
Kui Lin-Wang ◽  
Karen Bolitho ◽  
Karryn Grafton ◽  
Anne Kortstee ◽  
Sakuntala Karunairetnam ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Biosynthetic Pathway ◽  
Myb Transcription Factor ◽  
Anthocyanin Biosynthetic Pathway ◽  
R2r3 Myb

scholarly journals Characterization of BoaCRTISO Reveals Its Role in Carotenoid Biosynthesis in Chinese Kale

2021 ◽  
Vol 12 ◽  
Author(s):  
Min Jiang ◽  
Fen Zhang ◽  
Qiao Yuan ◽  
Peixing Lin ◽  
Hao Zheng ◽  
...  
Keyword(s):  
Acid Treatment ◽  
Biosynthetic Pathway ◽  
Developmental Stages ◽  
Functional Characterization ◽  
Carotenoid Biosynthesis ◽  
Virus Induced Gene Silencing ◽  
Strong Light ◽  
Organic Pigments ◽  
Chinese Kale

Carotenoids are organic pigments that play an important role in both plant coloration and human health; they are a critical subject in molecular breeding due to growing demand for natural molecules in both food and medicine. In this study, we focus upon characterizing BoaCRTISO, the carotenoid isomerase gene before the branch of the carotenoid biosynthetic pathway, which is expressed in all organs and developmental stages of Chinese kale, and BoaCRTISO, which is located in the chloroplast. The expression of BoaCRTISO is induced by strong light, red and blue combined light, and gibberellic acid treatment, but it is suppressed by darkness and abscisic acid treatment. We obtained BoaCRTISO-silenced plants via virus-induced gene silencing technology, and the silence efficiencies ranged from 52 to 77%. The expressions of most carotenoid and chlorophyll biosynthetic genes in BoaCRTISO-silenced plants were downregulated, and the contents of carotenoids and chlorophyll were reduced. Meanwhile, BoaCRTISO-silenced plants exhibited phenotypes of yellowing leaves and inhibited growth. This functional characterization of BoaCRTISO provides insight for the biosynthesis and regulation of carotenoid in Chinese kale.

Sign in / Sign up

Export Citation Format

Share Document