In vitro photoautotrophic acclimatization, direct transplantation and ex vitro adaptation of rubber tree (Hevea brasiliensis)

2017 ◽  
Vol 133 (2) ◽  
pp. 215-223 ◽  
Author(s):  
Rujira Tisarum ◽  
Thapanee Samphumphung ◽  
Cattarin Theerawitaya ◽  
Wittaya Prommee ◽  
Suriyan Cha-um
Keyword(s):  
Hevea Brasiliensis ◽  
Rubber Tree ◽  
Ex Vitro

scholarly journals Purification, characterization, and bioassay of putative protease inhibitors from Hevea brasiliensis latex

2017 ◽  
Vol 84 (2) ◽  
Author(s):  
Riza Arief PUTRANTO ◽  
. SISWANTO ◽  
Agustin Sri MULYATNI ◽  
Asmini BUDIANI ◽  
Radite TISTAMA
Keyword(s):  
Protease Inhibitor ◽  
Protease Inhibitors ◽  
Inhibitory Activity ◽  
Hevea Brasiliensis ◽  
Rubber Tree ◽  
Exchange Chromatography ◽  
Mycelium Growth ◽  
Plant Protease ◽  
Putative Protease

Latex, a milky white liquid, is the main product from rubber tree (Hevea brasiliensis). Latex is the cytoplasm of complex cellular networks named laticifers in which it contains many different components, including important proteins. Various types of enzymes carrying functions associated with plant defense against pathogen and wounding have been detected in latex in which one of these enzymes is protease inhibitor (PI). Plant protease inhibitor has tremendous potential as an antifungal agent which can be developed as biofungicide. In this work, protease inhibitors from B-serum (lutoid) of rubber tree latex were isolated and purified using Ion Exchange Chromatography (IEC) technique. Of the total 70 fractions of proteins extracted from the columns, only 26 fractions showed measurable levels of protein. The concentration of obtained putative protease inhibitors (three fractions of IEC) ranged from 0.007 to 0.022 mL/g B-serum. Inhibitory activity against four protease enzymes (subtilisin A, trypsin, α-chymotrypsin, and papain) showed the characteristics of Hevea putative protease inhibitors from B-serum as serine and/or cysteine protease inhibitors with more than 15% inhibitory activity of target protease. Based on SDS-PAGE visualization, the molecular weight of dominant protein considered as Hevea putative protease inhibitors was 21.5 kDa. In vitro bioassay test of antifungal activity for Hevea putative protease inhibitors showed reduced mycelium growth of Ganoderma boninense, Sclerotium sp., and Rigidosporus lignosus.

scholarly journals Daya hidup planlet karet asal in vitro microcutting pada berbagai periode penutupan sungkup plastik dan komposisi media tumbuh Survival rate of in vitro microcutting-derived rubber plantlets on various plastic cover closed periods and medium compositions

2016 ◽  
Vol 80 (1) ◽  
Author(s):  
. SUMARYONO ◽  
Masna Maya SINTA ◽  
. NURHAIMI-HARIS
Keyword(s):  
Survival Rate ◽  
Hevea Brasiliensis ◽  
Tree Canopy ◽  
Ex Vitro ◽  
Media Composition ◽  
Transparent Plastic ◽  
Growing Medium ◽  
Major Bottleneck ◽  
Better Than

AbstractIn vitro culture through microcutting technology can be used for clonal propagation of rubber (Hevea brasiliensis Muell. Arg.) rootstocks. Acclimatization of in vitro plantlets to ex vitro conditions is a major bottleneck in the micropropagation of many plants.This research was conducted to study the effect of plastic cover closed period and media composition on the survival rate of rubber plantlets. Plantlets derived from microcutting were planted on plastic pots containing a mixture of soil, cocopeat, dung manure, and sand or zeolite. The plantlets were then placed inside a closed transparent plastic cover that opened after 2, 3, 4 and 6 weeks. The cover was placed under tree canopy. The second experiment used the same media composition with or without cocopeat and with sand or zeolite. At 1.5 month after culture, observation was done on the number of survived plantlets, plantlet height and the percentage of rooted plantlets. The results show that the best coverclosed period was six weeks and the best growing medium was a mixture of soil, cocopeat, dung manure, and zeolite (6:2:1:1v/v). On the two combined treatments, the survival rate was 73.3% after 1.5 month of acclimatization. The use of zeolite and a higher soil percentage gave positive influences on rubber plantlet survival rate. The second experiment results confirmed that the use of zeolite was better than sand and the use of cocopeat was definitely needed. It can be concluded that the best of acclimatization of rubber plantlets from microcutting was on a medium mixture of soil, cocopeat, dung manure, and zeolite (6:2:1:1) and placed inside a closed plastic cover for six weeks before the cover was opened gradually. AbstrakKultur in vitro melalui teknologi microcutting dapat digunakan untuk perbanyakan klonal batang bawah tanaman karet (Hevea brasiliensis Muell. Arg.). Aklimatisasi planlet in vitro ke kondisi ex vitro merupakan hambatan utama pada mikropropagasi berbagai jenis tanaman. Penelitian ini dilakukan untuk mempelajari pengaruh lama penutupan sungkup plastik dan komposisi media tumbuh terhadap daya hidup planlet karet. Planlet karet asal microcutting ditanam pada pot plastik berisi media dengan berbagai campuran tanah, cocopeat, pupuk kandang, dan pasir atau zeolit. Planlet selanjutnya diletakkan di dalam sungkup plastik transparan tertutup rapat yang dibuka setelah 2, 3, 4 dan 6 minggu. Sungkup plastik diletakkan di bawah tajuk pepohonan. Percobaan kedua menggunakan komposisi media serupa dengan atau tanpa cocopeat dan dengan pasir atau zeolit. Pada umur 1,5 bulan, pengamatan dilakukan terhadap jumlah planlet yang hidup, tinggi planlet, dan persentase planlet yang berakar. Hasil penelitian menunjukkan bahwa lama penyungkupan terbaik adalah enam minggu dan media tumbuh terbaik adalah campuran tanah, cocopeat, pupuk kandang, dan zeolit (6:2:1:1 v/v). Pada kombinasi kedua perlakuan tersebut, daya hidup planlet karet mencapai 73,3% setelah 1,5 bulan aklimatisasi. Penggunaan zeolit dan persentase tanah yang lebih tinggi berpengaruh positif terhadap daya hidup planlet karet. Hasil percobaan kedua menegaskan bahwa penggunaan zeolit lebih baik daripada pasir dan penggunaan cocopeat mutlak diperlukan. Dapat disimpulkan bahwa aklimatisasi planlet karet asal microcutting terbaik dilakukan pada media campuran tanah, cocopeat, pupuk kandang, zeolit (6:2:1:1) dan diletakkan di dalam sungkup plastik tertutup selama enam minggu sebelum sungkup dibuka secara bertahap.

scholarly journals In vitro antioxidant extracts evaluation from the residue of the Hevea brasiliensis seed

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Giovanna Oleinik ◽  
Priscila Paola Dario ◽  
Katiane de Morais Gasperin ◽  
Dalila Moter Benvegnú ◽  
Fernanda Oliveira Lima ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Hevea Brasiliensis ◽  
Food Industry ◽  
Rubber Tree ◽  
Added Value ◽  
Future Research ◽  
Synthetic Antioxidants ◽  
Production Chain

AbstractThe antioxidants used in the food industry are essential to inhibit the formation of free radicals, preserving the existing properties in the different matrices. However, the insecurity of the synthetic antioxidants regarding human health propels search for natural substrates with potential antioxidant activity as an alternative to synthetic compounds. In this way, the work had as objective obtaining extracts from the seed pomace of the Hevea brasiliensis (rubber tree), relating the contents of flavonoids and total phenols in the application as an antioxidant. The methodology consisted of the extraction using four solvents, varying extractive methods, time, and seed concentrations. The antioxidant activity in vitro was evaluated by capturing the DPPH (2,2-diphenyl-1-picryl-hydrazil) radical. The optimized results demonstrate that the aqueous extracts produced in the Soxhlet in the concentrations of 85 g L−1 and retention time of 4 h reached 37.73 ± 1.69% in the antioxidant tests of the free radical DPPH capture, 1405.15 mg EAC 100 g−1 in the quantification of phenolic compounds and 223.34 mg 100 g−1 of total flavonoids. Thus, this work may contribute to the realization of studies and future research for characterization and identification concerning which phenolic compounds and flavonoids attribute the antioxidant characteristic to the extracts produced, enabling the discovery of products with high added value in the production chain. In addition, because the water used as a solvent showed greater antioxidant potential between the extracts, the non-toxic and environmentally friendly character is highlighted, allowing a wide variety of applications in the food industry.

In vitro mycorrhization of the rubber tree Hevea brasiliensis Müll Arg

2013 ◽  
Vol 49 (2) ◽  
pp. 207-215 ◽  
Author(s):  
Tiffany Sosa-Rodriguez ◽  
Hervé Dupré de Boulois ◽  
Françoise Granet ◽  
Sylvie Gaurel ◽  
Luz-Marina Melgarejo ◽  
...  
Keyword(s):  
Hevea Brasiliensis ◽  
Rubber Tree

scholarly journals Ex Vitro Propagation of Rubber Tree (Hevea Brasiliensis) using Stem Cuttings

2018 ◽  
Vol 3 (3) ◽  
pp. 846-854
Author(s):  
Anthony Antwi-Wiredu ◽  
Samuel Amiteye ◽  
Rhoda Gyinae Diawuoh ◽  
George Y. P. Klu
Keyword(s):  
Hevea Brasiliensis ◽  
Rubber Tree ◽  
Stem Cuttings ◽  
Ex Vitro

scholarly journals Pengaruh periode pra-kondisi dan penutupan sungkup terhadap daya hidup planlet karet (Hevea brasiliensis Muell. Arg) Effect of pre-condition period and vessel closure on the survival rate of rubber (Hevea brasiliensis Muell. Arg) plantlets

2016 ◽  
Vol 81 (1) ◽  
Author(s):  
Masna Maya SINTA ◽  
. NURHAIMI-HARIS ◽  
. SUMARYONO
Keyword(s):  
Survival Rate ◽  
Hevea Brasiliensis ◽  
Glass Container ◽  
Ex Vitro ◽  
Critical Stage ◽  
Plastic Container ◽  
Growth And Survival ◽  
Vessel Closure ◽  
Increase Survival Rate

AbstractAcclimatization of plantlets is a critical stage in themicropropagation of many plants. An experiment wasconducted to determine the effect of pre-condition periodand vessel closure on the growth and survival rate ofrubber (Hevea brasiliensis Muell.Arg.) plantlets derivedfrom in vitro microcutting during acclimatization.Plantlets were planted in plastic pots containing mixedgrowing media after being conditioned in ex vitroenvironment for 0, 3 and 6 days. Five closure vesseltreatments were closed pots placed in opened container,opened pots in closed glass container, closed pots inclosed glass container, opened pots in closed plasticcontainer, and closed pots in closed plastic container.Observation on leaf conditions, rooting frequency, andplant height were conducted at 1.5 months and on thepercentage of survive plantlets at 1.5 and 3 months afteracclimatization. The results showed that pre-conditionwas required to increase survival rate and growth of theplantlets. Pre-condition period of six days gave a highersurvival rate than 0 and 3 days which reached 100% and93% on opened pot in closed plastic container and closedpot in opened container, respectively after 1.5 monthsand was reduced to 80% after three months ofacclimatization. The highest formation of new leaves androots were also obtained on six days pre-conditionperiod. Plantlets with pre-condition for six days and wereplanted on closed pots in an opened container had thebest rooting frequency which was 90%. The resultshowed that the highest survival rate (80%) of rubberplantlets after three months was obtained when theplantlets were pre-conditioned in ex vitro conditions forsix days before acclimatization and planted on openedpots in a closed plastic container or closed pots in anopened container.AbstrakAklimatisasi planlet merupakan tahap kritis dalammikropopagasi tanaman. Penelitian dilakukan untuk me-nentukan pengaruh periode pra-kondisi dan penyung-kupan terhadap pertumbuhan dan daya hidup planletkaret (Hevea brasiliensis Muell.Arg.) asal stek mikro (invitro microcutting) selama aklimatisasi. Planlet ditanampada pot plastik berisi campuran media tanam setelahdikondisikan lebih dahulu pada lingkungan luar selama 0,3 dan 6 hari. Lima perlakuan penyungkupan adalahpenanaman planlet pada pot tertutup diletakkan dalamwadah terbuka, pot terbuka dalam wadah kaca tertutup,pot tertutup dalam wadah kaca tertutup, pot terbukadalam wadah plastik tertutup dan pot tertutup dalamwadah plastik tertutup. Pengamatan keadaan daun, pem-bentukan akar dan tinggi tanaman dilakukan pada 1,5bulan, sedangkan persentase planlet yang hidup diamatipada 1,5 dan 3 bulan setelah aklimatisasi. Hasil penelitianmenunjukkan bahwa periode pra-kondisi diperlukanuntuk meningkatkan daya hidup dan pertumbuhanplanlet. Pra-kondisi selama enam hari memberikan dayahidup planlet lebih tinggi dibandingkan dengan 0 dan 3hari yaitu 100% dan 93% pada perlakuan penanamanpada pot terbuka dalam wadah plastik tertutup dan pottertutup dalam wadah terbuka setelah 1,5 bulan danmenjadi 80% setelah tiga bulan. Penambahan daun barudan pembentukan akar tertinggi juga terdapat padaperlakuan pra-kondisi enam hari. Perlakuan pra-kondisienam hari dengan pot tertutup yang diletakkan dalamwadah terbuka memperlihatkan persentase pembentukanakar yang paling baik yakni 90%. Hasil peneltian me-nunjukkan bahwa daya hidup planlet karet tertinggi(80%) pada umur tiga bulan diperoleh apabila planletdipra-kondisi pada lingkungan ex vitro selama enam hari,kemudian ditanam pada pot terbuka dalam wadah plastiktertutup atau pot tertutup dalam wadah terbuka.

scholarly journals Purification, characterization, and bioassay of putative protease inhibitors from Hevea brasiliensis latex

2017 ◽  
Vol 84 (2) ◽  
Author(s):  
Riza Arief PUTRANTO ◽  
. SISWANTO ◽  
Agustin Sri MULYATNI ◽  
Asmini BUDIANI ◽  
Radite TISTAMA
Keyword(s):  
Ion Exchange ◽  
Protease Inhibitor ◽  
Protease Inhibitors ◽  
Hevea Brasiliensis ◽  
Rubber Tree ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Sds Page ◽  
Putative Protease

Lateks yang menyerupai cairan susu putih diperoleh dari penyadapan kulit batang tanaman karet (Hevea brasiliensis). Lateks merupakan sitoplasma dari jaringan pembuluh bernama latisifer yang didalamnya terkandung berbagai macam komponen, termasuk protein-protein penting. Berbagai jenis enzim yang memiliki fungsi terkait pertahanan tanaman dari serangan patogen dan pelukaan telah berhasil dideteksi di dalam lateks, di antaranya protease inhibitor (PI). Protease inhibitor memiliki aktivitas senyawa antifungi sehingga berpotensi untuk  dimanfaatkan sebagai biofungisida. Pada penelitian ini, protease  inhibitor putatif yang berasal dari serum B (lutoid) lateks tanaman karet telah berhasil diisolasi menggunakan teknik Ion Exchange Chroma-tography. Dari total 70 fraksi protein yang diekstrak dari kolom, hanya 26 fraksi yang menunjukkan kadar protein yang terukur. Kandungan protease inhibitor putatif yang di-peroleh berkisar antara 0,0067 hingga 0,022 mL/g serum B dari hasil 3 fraksi terpilih. Aktivitas penghambatan terhadap empat enzim protease (subtilisin A, tripsin, α-kimotripsin, dan papain) menunjukkan karakteristik protease inhibitor putatif tersebut sebagai serine dan/atau cysteine inhibitor protease dengan persentase hambatan di atas 15% terhadap protease target. Hasil SDS-PAGE memperlihatkan pemisahan protein dominan yang diperkirakan merupakan protease inhibitor putatif dengan berat molekul sebesar 21,5 kDa. Uji bioassay aktivitas antifungi secara in vitro dari protease inhibitor memperlihatkan penghambatan pertumbuhan miselium dari fungi Ganoderma boninense, Sclerotium sp., dan Rigidosporus lignosus. [Kata kunci : protease inhibitor, Hevea brasiliensis, lateks, serum B, ion exchange chromatography]AbstractLatex, a milky white liquid, is the main product from rubber tree (Hevea brasiliensis). Latex is the cytoplasm of complex cellular networks named laticifers in which it contains many different components, including important proteins. Various types of enzymes carrying functions associated with plant defense against pathogen and wounding have been detected in latex in which one of these enzymes is protease inhibitor (PI). Plant protease inhibitor has tremendous potential as an antifungal agent which can be developed as biofungicide. In this work, protease inhibitors from B-serum (lutoid) of rubber tree latex were isolated and purified using Ion Exchange Chromatography (IEC) technique. Of the total 70 fractions of proteins extracted from the columns, only 26 fractions showed measurable levels of protein. The concentration of obtained putative protease inhibitors (three fractions of IEC) ranged from 0.007 to 0.022 mL/g B-serum. Inhibitory activity against four protease enzymes (subtilisin A, trypsin, α-chymotrypsin, and papain) showed the characteristics of Hevea putative protease inhibitors from B-serum as serine and/or cysteine protease inhibitors with more than 15% inhibitory activity of target protease. Based on SDS-PAGE visualization, the molecular weight of dominant protein considered as Hevea putative protease inhibitors was 21.5 kDa. In vitro bioassay test of antifungal activity for Hevea putative protease inhibitors showed reduced mycelium growth of Ganoderma boninense, Sclerotium sp., and Rigidosporus lignosus.[Keywords: protease inhibitor, Hevea brasiliensis, latex, B-serum, ion exchange chromatography]

scholarly journals Pengaruh periode pra-kondisi dan penutupan sungkup terhadap daya hidup planlet karet (Hevea brasiliensis Muell. Arg) Effect of pre-condition period and vessel closure on the survival rate of rubber (Hevea brasiliensis Muell. Arg) plantlets

2016 ◽  
Vol 81 (1) ◽  
Author(s):  
Masna Maya SINTA ◽  
. NURHAIMI-HARIS ◽  
. SUMARYONO
Keyword(s):  
Survival Rate ◽  
Hevea Brasiliensis ◽  
Glass Container ◽  
Ex Vitro ◽  
Critical Stage ◽  
Plastic Container ◽  
Growth And Survival ◽  
Vessel Closure ◽  
Increase Survival Rate

AbstractAcclimatization of plantlets is a critical stage in themicropropagation of many plants. An experiment wasconducted to determine the effect of pre-condition periodand vessel closure on the growth and survival rate ofrubber (Hevea brasiliensis Muell.Arg.) plantlets derivedfrom in vitro microcutting during acclimatization.Plantlets were planted in plastic pots containing mixedgrowing media after being conditioned in ex vitroenvironment for 0, 3 and 6 days. Five closure vesseltreatments were closed pots placed in opened container,opened pots in closed glass container, closed pots inclosed glass container, opened pots in closed plasticcontainer, and closed pots in closed plastic container.Observation on leaf conditions, rooting frequency, andplant height were conducted at 1.5 months and on thepercentage of survive plantlets at 1.5 and 3 months afteracclimatization. The results showed that pre-conditionwas required to increase survival rate and growth of theplantlets. Pre-condition period of six days gave a highersurvival rate than 0 and 3 days which reached 100% and93% on opened pot in closed plastic container and closedpot in opened container, respectively after 1.5 monthsand was reduced to 80% after three months ofacclimatization. The highest formation of new leaves androots were also obtained on six days pre-conditionperiod. Plantlets with pre-condition for six days and wereplanted on closed pots in an opened container had thebest rooting frequency which was 90%. The resultshowed that the highest survival rate (80%) of rubberplantlets after three months was obtained when theplantlets were pre-conditioned in ex vitro conditions forsix days before acclimatization and planted on openedpots in a closed plastic container or closed pots in anopened container.AbstrakAklimatisasi planlet merupakan tahap kritis dalammikropopagasi tanaman. Penelitian dilakukan untuk me-nentukan pengaruh periode pra-kondisi dan penyung-kupan terhadap pertumbuhan dan daya hidup planletkaret (Hevea brasiliensis Muell.Arg.) asal stek mikro (invitro microcutting) selama aklimatisasi. Planlet ditanampada pot plastik berisi campuran media tanam setelahdikondisikan lebih dahulu pada lingkungan luar selama 0,3 dan 6 hari. Lima perlakuan penyungkupan adalahpenanaman planlet pada pot tertutup diletakkan dalamwadah terbuka, pot terbuka dalam wadah kaca tertutup,pot tertutup dalam wadah kaca tertutup, pot terbukadalam wadah plastik tertutup dan pot tertutup dalamwadah plastik tertutup. Pengamatan keadaan daun, pem-bentukan akar dan tinggi tanaman dilakukan pada 1,5bulan, sedangkan persentase planlet yang hidup diamatipada 1,5 dan 3 bulan setelah aklimatisasi. Hasil penelitianmenunjukkan bahwa periode pra-kondisi diperlukanuntuk meningkatkan daya hidup dan pertumbuhanplanlet. Pra-kondisi selama enam hari memberikan dayahidup planlet lebih tinggi dibandingkan dengan 0 dan 3hari yaitu 100% dan 93% pada perlakuan penanamanpada pot terbuka dalam wadah plastik tertutup dan pottertutup dalam wadah terbuka setelah 1,5 bulan danmenjadi 80% setelah tiga bulan. Penambahan daun barudan pembentukan akar tertinggi juga terdapat padaperlakuan pra-kondisi enam hari. Perlakuan pra-kondisienam hari dengan pot tertutup yang diletakkan dalamwadah terbuka memperlihatkan persentase pembentukanakar yang paling baik yakni 90%. Hasil peneltian me-nunjukkan bahwa daya hidup planlet karet tertinggi(80%) pada umur tiga bulan diperoleh apabila planletdipra-kondisi pada lingkungan ex vitro selama enam hari,kemudian ditanam pada pot terbuka dalam wadah plastiktertutup atau pot tertutup dalam wadah terbuka.

scholarly journals In Vitro Propagation of Rubber Tree (Hevea Brasiliensis) Using Shoot-Tip and Nodal Cutting Explants

2018 ◽  
Vol 4 (6) ◽  
pp. 38-50
Author(s):  
Anthony Antwi-Wiredu ◽  
Samuel Amiteye ◽  
Rhoda Gyinae Diawuoh ◽  
Alex Kofi Asumeng ◽  
George Y. P. Klu
Keyword(s):  
Hevea Brasiliensis ◽  
In Vitro Propagation ◽  
Rubber Tree ◽  
Shoot Tip ◽  
Nodal Cutting

scholarly journals Daya hidup planlet karet asal in vitro microcutting pada berbagai periode penutupan sungkup plastik dan komposisi media tumbuh Survival rate of in vitro microcutting-derived rubber plantlets on various plastic cover closed periods and medium compositions

2016 ◽  
Vol 80 (1) ◽  
Author(s):  
. SUMARYONO ◽  
Masna Maya SINTA ◽  
. NURHAIMI-HARIS
Keyword(s):  
Survival Rate ◽  
Hevea Brasiliensis ◽  
Tree Canopy ◽  
Ex Vitro ◽  
Media Composition ◽  
Transparent Plastic ◽  
Growing Medium ◽  
Major Bottleneck ◽  
Better Than

AbstractIn vitro culture through microcutting technology can be used for clonal propagation of rubber (Hevea brasiliensis Muell. Arg.) rootstocks. Acclimatization of in vitro plantlets to ex vitro conditions is a major bottleneck in the micropropagation of many plants.This research was conducted to study the effect of plastic cover closed period and media composition on the survival rate of rubber plantlets. Plantlets derived from microcutting were planted on plastic pots containing a mixture of soil, cocopeat, dung manure, and sand or zeolite. The plantlets were then placed inside a closed transparent plastic cover that opened after 2, 3, 4 and 6 weeks. The cover was placed under tree canopy. The second experiment used the same media composition with or without cocopeat and with sand or zeolite. At 1.5 month after culture, observation was done on the number of survived plantlets, plantlet height and the percentage of rooted plantlets. The results show that the best coverclosed period was six weeks and the best growing medium was a mixture of soil, cocopeat, dung manure, and zeolite (6:2:1:1v/v). On the two combined treatments, the survival rate was 73.3% after 1.5 month of acclimatization. The use of zeolite and a higher soil percentage gave positive influences on rubber plantlet survival rate. The second experiment results confirmed that the use of zeolite was better than sand and the use of cocopeat was definitely needed. It can be concluded that the best of acclimatization of rubber plantlets from microcutting was on a medium mixture of soil, cocopeat, dung manure, and zeolite (6:2:1:1) and placed inside a closed plastic cover for six weeks before the cover was opened gradually. AbstrakKultur in vitro melalui teknologi microcutting dapat digunakan untuk perbanyakan klonal batang bawah tanaman karet (Hevea brasiliensis Muell. Arg.). Aklimatisasi planlet in vitro ke kondisi ex vitro merupakan hambatan utama pada mikropropagasi berbagai jenis tanaman. Penelitian ini dilakukan untuk mempelajari pengaruh lama penutupan sungkup plastik dan komposisi media tumbuh terhadap daya hidup planlet karet. Planlet karet asal microcutting ditanam pada pot plastik berisi media dengan berbagai campuran tanah, cocopeat, pupuk kandang, dan pasir atau zeolit. Planlet selanjutnya diletakkan di dalam sungkup plastik transparan tertutup rapat yang dibuka setelah 2, 3, 4 dan 6 minggu. Sungkup plastik diletakkan di bawah tajuk pepohonan. Percobaan kedua menggunakan komposisi media serupa dengan atau tanpa cocopeat dan dengan pasir atau zeolit. Pada umur 1,5 bulan, pengamatan dilakukan terhadap jumlah planlet yang hidup, tinggi planlet, dan persentase planlet yang berakar. Hasil penelitian menunjukkan bahwa lama penyungkupan terbaik adalah enam minggu dan media tumbuh terbaik adalah campuran tanah, cocopeat, pupuk kandang, dan zeolit (6:2:1:1 v/v). Pada kombinasi kedua perlakuan tersebut, daya hidup planlet karet mencapai 73,3% setelah 1,5 bulan aklimatisasi. Penggunaan zeolit dan persentase tanah yang lebih tinggi berpengaruh positif terhadap daya hidup planlet karet. Hasil percobaan kedua menegaskan bahwa penggunaan zeolit lebih baik daripada pasir dan penggunaan cocopeat mutlak diperlukan. Dapat disimpulkan bahwa aklimatisasi planlet karet asal microcutting terbaik dilakukan pada media campuran tanah, cocopeat, pupuk kandang, zeolit (6:2:1:1) dan diletakkan di dalam sungkup plastik tertutup selama enam minggu sebelum sungkup dibuka secara bertahap.

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