Drought stress-induced methyl jasmonate accumulation promotes calycosin-7-O-β-d-glucoside production in Astragalus membranaceus adventitious roots

2021 ◽  
Author(s):  
Yichuan Feng ◽  
Yang Zhao ◽  
Yang Ha ◽  
Junjie Li ◽  
Zheying Su ◽  
...  
Keyword(s):  
Drought Stress ◽  
Methyl Jasmonate ◽  
Adventitious Roots ◽  
Astragalus Membranaceus

scholarly journals Response of Bioactive Metabolite and Biosynthesis Related Genes to Methyl Jasmonate Elicitation in Codonopsis pilosula

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 533 ◽  
Author(s):  
Jiao-jiao Ji ◽  
Qi Feng ◽  
Hai-feng Sun ◽  
Xue-jun Zhang ◽  
Xiao-xiao Li ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Adventitious Roots ◽  
Multiple Shoots ◽  
Bioactive Metabolites ◽  
Bioactive Metabolite ◽  
Codonopsis Pilosula ◽  
Meja Treatment ◽  
Metabolite Accumulation ◽  
Culture Growth ◽  
Sucrose Level

Bioactive metabolites in Codonopsis pilosula are of particular interest as an immunostimulant. Methyl jasmonate (MeJA) plays an important role in the elicitation of metabolite biosynthesis. Here, we explored the response of metabolites to MeJA elicitation in C. pilosula adventitious roots and multiple shoots. The results showed that the biomass, polysaccharide, and lobetyolin content of adventitious roots exhibited the highest increases with 100 µmol·L−1 MeJA at the 16th day of subculture, whereas the atractylenolide III (a terpenoid) content increased extremely with 50 µmol·L−1 MeJA treatment at the 7th day of subculture. In addition, the biomass and lobetyolin content significantly increased at the 4th day after treatment. Similarly, the polysaccharide and lobetyolin content increased in multiple shoots. Further identification of different metabolites responding to MeJA by 1H-NMR showed an extremely significant increase of the lobetyolinin level, which coincided with lobetyolin. Accordingly, the precursor, fatty acids, showed a highly significant decrease in their levels. Furthermore, a significant increase in β-d-fructose-butanol glycoside was detected, which was accompanied by a decrease in the sucrose level. Accordingly, the enzyme genes responsible for terpenoid and carbohydrate biosynthesis, CpUGPase, and CpPMK, were up regulated. In conclusion, MeJA promoted culture growth and accelerated bioactive metabolite accumulation by regulating the expression of the metabolite biosynthesis related genes, CpUGPase and CpPMK in C. pilosula.

Triterpene and Flavonoid Biosynthesis and Metabolic Profiling of Hairy Roots, Adventitious Roots, and Seedling Roots of Astragalus membranaceus

2015 ◽  
Vol 63 (40) ◽  
pp. 8862-8869 ◽  
Author(s):  
Yun Ji Park ◽  
Aye Aye Thwe ◽  
Xiaohua Li ◽  
Yeon Jeong Kim ◽  
Jae Kwang Kim ◽  
...  
Keyword(s):  
Hairy Roots ◽  
Metabolic Profiling ◽  
Adventitious Roots ◽  
Flavonoid Biosynthesis ◽  
Astragalus Membranaceus ◽  
Seedling Roots

Alleviation of drought stress and the physiological mechanisms in Citrus cultivar (Huangguogan) treated with methyl jasmonate

2020 ◽  
Vol 84 (9) ◽  
pp. 1958-1965 ◽  
Author(s):  
Bo Xiong ◽  
Yuan Wang ◽  
Yue Zhang ◽  
Mengmeng Ma ◽  
Yifei Gao ◽  
...  
Keyword(s):  
Drought Stress ◽  
Methyl Jasmonate ◽  
Physiological Mechanisms ◽  
Citrus Cultivar
Sign in / Sign up

Export Citation Format

Share Document