Triterpene and Flavonoid Biosynthesis and Metabolic Profiling of Hairy Roots, Adventitious Roots, and Seedling Roots of Astragalus membranaceus

2015 ◽  
Vol 63 (40) ◽  
pp. 8862-8869 ◽  
Author(s):  
Yun Ji Park ◽  
Aye Aye Thwe ◽  
Xiaohua Li ◽  
Yeon Jeong Kim ◽  
Jae Kwang Kim ◽  
...  
Keyword(s):  
Hairy Roots ◽  
Metabolic Profiling ◽  
Adventitious Roots ◽  
Flavonoid Biosynthesis ◽  
Astragalus Membranaceus ◽  
Seedling Roots

scholarly journals Resveratrol Biosynthesis in Hairy Root Cultures of Tan and Purple Seed Coat Peanuts

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 975
Author(s):  
Ye-Eun Park ◽  
Chang-Ha Park ◽  
Hyeon-Ji Yeo ◽  
Yong-Suk Chung ◽  
Sang-Un Park
Keyword(s):  
Arachis Hypogaea ◽  
Hairy Root ◽  
Hairy Roots ◽  
Seed Coat ◽  
Biological Properties ◽  
Fresh Weight ◽  
Good Source ◽  
Plant Parts ◽  
Seedling Roots

Peanut (Arachis hypogaea) is a crop that can produce resveratrol, a compound with various biological properties, such as those that exert antioxidant, anticancer, and anti-inflammatory effects. In this study, trans-resveratrol was detected in the roots, leaves, and stems of tan and purple seed coat peanuts (Arachis hypogaea) cultivated in a growth chamber. Both cultivars showed higher levels of resveratrol in the roots than the other plant parts. Thus, both cultivars were inoculated with Agrobacterium rhizogenes, in vitro, to promote hairy root development, thereby producing enhanced levels of t-resveratrol. After 1 month of culture, hairy roots from the two cultivars showed higher levels of fresh weight than those of seedling roots. Furthermore, both cultivars contained higher t-resveratrol levels than those of their seedling roots (6.88 ± 0.21 mg/g and 28.07 ± 0.46 mg/g, respectively); however, purple seed coat peanut hairy roots contained higher t-resveratrol levels than those of tan seed coat peanut hairy roots, ranging from 70.16 to 166.76 mg/g and from 46.61 to 54.31 mg/g, respectively. The findings of this study indicate that peanut hairy roots could be a good source for t-resveratrol production due to their rapid growth, high biomass, and substantial amount of resveratrol.

Metabolic Profiling and Transcriptome Analysis of Mulberry Leaves Provide Insights into Flavonoid Biosynthesis

2020 ◽  
Vol 68 (5) ◽  
pp. 1494-1504 ◽  
Author(s):  
Dong Li ◽  
Guo Chen ◽  
Bi Ma ◽  
Chengzhang Zhong ◽  
Ningjia He
Keyword(s):  
Transcriptome Analysis ◽  
Metabolic Profiling ◽  
Flavonoid Biosynthesis ◽  
Mulberry Leaves

scholarly journals Comparison of morphogenesis and anatomy between hairy roots and adventitious roots of Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv.)

2020 ◽  
Vol 17 (3) ◽  
pp. 483-490
Author(s):  
Trinh Thi Huong ◽  
Nguyen Thi Nhat Linh ◽  
Hoang Thanh Tung ◽  
Vu Thi Hien ◽  
Vu Quoc Luan ◽  
...  
Keyword(s):  
Hairy Roots ◽  
Adventitious Roots

scholarly journals Effect of Light and Dark on the Phenolic Compound Accumulation in Tartary Buckwheat Hairy Roots Overexpressing ZmLC

2021 ◽  
Vol 22 (9) ◽  
pp. 4702
Author(s):  
Chang Ha Park ◽  
Ye Eun Park ◽  
Hyeon Ji Yeo ◽  
Nam Il Park ◽  
Sang Un Park
Keyword(s):  
Transcription Factor ◽  
Phenolic Compounds ◽  
Hairy Roots ◽  
Sinapic Acid ◽  
Dry Weight ◽  
Flavonoid Biosynthesis ◽  
Total Phenolic ◽  
Fagopyrum Tataricum ◽  
Continuous Darkness ◽  
Phenolic Contents

Fagopyrum tataricum ‘Hokkai T10′ is a buckwheat cultivar capable of producing large amounts of phenolic compounds, including flavonoids (anthocyanins), phenolic acids, and catechin, which have antioxidant, anticancer, and anti-inflammatory properties. In the present study, we revealed that the maize transcription factor Lc increased the accumulation of phenolic compounds, including sinapic acid, 4-hydroxybenzonate, t-cinnamic acid, and rutin, in Hokkai T10 hairy roots cultured under long-photoperiod (16 h light and 8 h dark) conditions. The transcription factor upregulated phenylpropanoid and flavonoid biosynthesis pathway genes, yielding total phenolic contents reaching 27.0 ± 3.30 mg g−1 dry weight, 163% greater than the total flavonoid content produced by a GUS-overexpressing line (control). In contrast, when cultured under continuous darkness, the phenolic accumulation was not significantly different between the ZmLC-overexpressing hairy roots and the control. These findings suggest that the transcription factor (ZmLC) activity may be light-responsive in the ZmLC-overexpressing hairy roots of F. tataricum, triggering activation of the phenylpropanoid and flavonoid biosynthesis pathways. Further studies are required on the optimization of light intensity in ZmLC-overexpressing hairy roots of F. tataricum to enhance the production of phenolic compounds.

Optimization of Astragalus membranaceus hairy roots induction and culture conditions for augmentation production of astragalosides

2014 ◽  
Vol 120 (3) ◽  
pp. 1117-1130 ◽  
Author(s):  
Jiao Jiao ◽  
Qing-Yan Gai ◽  
Yu-Jie Fu ◽  
Wei Ma ◽  
Li-Ping Yao ◽  
...  
Keyword(s):  
Hairy Roots ◽  
Culture Conditions ◽  
Astragalus Membranaceus

scholarly journals Overexpression of AhHDA1 Results in Retarded Growth of Hairy Roots and Altered Cell Metabolism in Arachis hypogaea

2018 ◽  
Author(s):  
Liangchen Su ◽  
Shuai Liu ◽  
Xing Liu ◽  
Baihong Zhang ◽  
Meijuan Li ◽  
...  
Keyword(s):  
Arachis Hypogaea ◽  
Hairy Roots ◽  
Stress Responses ◽  
Metabolic Pathways ◽  
Cell Metabolism ◽  
Flavonoid Biosynthesis ◽  
In Vitro Cultivation ◽  
Biosynthesis Pathway ◽  
Histone Deacetylase 1

Peanut (Arachis hypogaea) is a crop plant with high economic value, but the epigenetic regulation of its growth and development has only rarely been studied. The peanut histone deacetylase 1 gene (AhHDA1) has been isolated and is known to be ABA- and drought-responsive. In this paper, we investigate the role of AhHDA1 in more detail, focussing on the effect of altered AhHDA1 expression in hairy roots at both the phenotypic and transcriptional levels. Agrobacterium rhizogenes-mediated transformation of A. hypogaea hairy roots was used to analyse how overexpression or RNA interference of AhHDA1 affects this tissue. In both types of transgenic hairy root, RNA sequencing was adopted to identify genes that were differentially expressed, and these genes were assigned to specific metabolic pathways. AhHDA1-overexpressing hairy roots were growth-retarded after 20 d in vitro cultivation, and superoxide anions and hydrogen peroxide accumulated to a greater extent than in control or RNAi groups. Overexpression of AhHDA1 is likely to accelerate flux through various secondary synthetic metabolic pathways in hairy roots, as well as reduce photosynthesis and oxidative phosphorylation. Genes encoding the critical enzymes caffeoyl-CoA O-methyltransferase (Araip.XGB85) and caffeic acid 3-O-methyltransferase (Araip.Z3XZX) in the phenylpropanoid biosynthesis pathway, chalcone synthase (Araip.B8TJ0) and polyketide reductase (Araip.MKZ27) in the flavonoid biosynthesis pathway, and hydroxyisoflavanone synthase (Araip.0P3RJ) and isoflavone 2'-hydroxylase (Araip.S5EJ7) in the isoflavonoid biosynthesis pathway were significantly upregulated by AhHDA1 overexpression, while their expression in AhHDA1-RNAi and control hairy roots remained at a lower level or was unchanged. Our results suggest that alteration of secondary metabolism activities is related to overexpression of AhHDA1, which is mainly reflected in phenylpropanoid, flavonoid and flavonoid biosynthesis. Future studies will focus on the function of AhHDA1 interacting proteins and their action on cell growth and stress responses.

scholarly journals UHPLC-HRMSn Analysis Reveals the Dynamic Metabonomic Responses of Salvia miltiorrhiza Hairy Roots to Polysaccharide Fraction from Trichoderma atroviride

Biomolecules ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 541 ◽  
Author(s):  
Ming ◽  
Dong ◽  
Wu ◽  
Zhu ◽  
Jia ◽  
...  
Keyword(s):  
Hairy Root ◽  
Hairy Roots ◽  
Clustering Analysis ◽  
Metabolic Profiling ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Salvia Miltiorrhiza ◽  
Principal Component ◽  
Trichoderma Atroviride ◽  
Polysaccharide Fraction

We have previously reported that Trichoderma atroviride, an endophytic fungus isolated from S. miltiorrhiza, promotes S. miltiorrhiza hairy root growth and significantly stimulates the biosynthesis of tanshinones specifically the polysaccharide fraction (PSF). However, this study only focused exclusively on six metabolites whilst ignoring changes to the whole metabolite composition of the S. miltiorrhiza hairy roots. In the present study, the dynamic metabonomic responses of S. miltiorrhiza hairy roots were investigated using ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn). UHPLC-HRMS typical total ions chromatograms (TICs) of PSF-treated hairy root samples were different from the control. Moreover, the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) indicated that PSF-treated samples were significantly different from the control. Through the analysis of PLS-DA, a total of 114 and 99 differential metabolites were found from the positive and negative models respectively and a total of 33 differential metabolites were identified. Thus, S. miltiorrhiza hairy roots had been induced to regulate the metabolic profiling in response to PSF and the changes of the metabolic profiling contributed to promoting the biosynthesis of tanshinones notably whilst the biosynthesis of phenolic acids were slightly inhibited.

Expression profile of a PAL gene from Astragalus membranaceus var. Mongholicus and its crucial role in flux into flavonoid biosynthesis

2006 ◽  
Vol 25 (7) ◽  
pp. 705-710 ◽  
Author(s):  
Rongrong Liu ◽  
Shaohua Xu ◽  
Jialin Li ◽  
Yuanlei Hu ◽  
Zhongping Lin
Keyword(s):  
Expression Profile ◽  
Crucial Role ◽  
Flavonoid Biosynthesis ◽  
Astragalus Membranaceus

scholarly journals LC/MS profiling of flavonoid glycoconjugates isolated from hairy roots, suspension root cell cultures and seedling roots of Medicago truncatula

Metabolomics ◽  
2011 ◽  
Vol 7 (4) ◽  
pp. 604-613 ◽  
Author(s):  
Anna Staszków ◽  
Barbara Swarcewicz ◽  
Joanna Banasiak ◽  
Dorota Muth ◽  
Michał Jasiński ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Cell Cultures ◽  
Hairy Roots ◽  
Root Cell ◽  
Seedling Roots
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