RNA inhibition of the JAZ9 gene increases the production of resveratrol in grape cell cultures

2021 ◽  
Author(s):  
Dmitrii S. Makhazen ◽  
G. N. Veremeichik ◽  
Y. N. Shkryl ◽  
V. P. Grigorchuk ◽  
G. K. Tchernoded ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Rna Inhibition ◽  
Grape Cell

ABA Initiates Anthocyanin Production in Grape Cell Cultures

2010 ◽  
Vol 30 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Séverine Gagné ◽  
Stéphanie Cluzet ◽  
Jean-Michel Mérillon ◽  
Laurence Gény
Keyword(s):  
Cell Cultures ◽  
Anthocyanin Production ◽  
Grape Cell

Induction of secondary metabolism in grape cell cultures by jasmonates

2009 ◽  
Vol 36 (4) ◽  
pp. 323 ◽  
Author(s):  
Claudio D' Onofrio ◽  
Agnieszka Cox ◽  
Christopher Davies ◽  
Paul K. Boss
Keyword(s):  
Secondary Metabolites ◽  
Cell Cultures ◽  
Mass Spectra ◽  
Genetic Approach ◽  
Microarray Experiments ◽  
Expression Of Genes ◽  
Generation Times ◽  
Meja Treatment ◽  
Grape Cell ◽  
Stilbene Biosynthesis

The use of a genetic approach to study the biosynthetic pathways leading to the production of secondary metabolites in grapes is difficult given the long generation times and difficulty in transforming this species. In the present study, GC/MS and microarray experiments were used to identify compounds produced in grape cell cultures in response to jasmonates and to examine the expression of genes from pathways that produce these secondary metabolites. Methyl jasmonate (MeJA) and jasmonic acid (JA) treatments resulted in the production of at least 25 compounds with sesquiterpene-like mass spectra in the cell cultures. A significantly greater amount of proanthocyanidins was produced in the MeJA-treated cell cultures compared with controls and stilbene biosynthesis was induced in both MeJA- and JA-treated cells. Salicylic acid (SA) suppressed the MeJA-associated increase in sesquiterpenes and proanthocyanidins, but SA did not suppress the stilbene production induced by MeJA treatment. The mechanism by which jasmonates induced secondary metabolite production in cultured grape cells varied depending on the pathway. The increased production of proanthocyanidins and stilbenes was associated with the induction of all of the genes in associated biosynthesis pathways, including those involved in the production of phenylalanine, whereas increased sesquiterpene synthesis was linked to the induction of certain genes from relevant biosynthesis pathways.

Enhanced anthocyanin production in grape cell cultures

Plant Science ◽  
1991 ◽  
Vol 78 (1) ◽  
pp. 107-120 ◽  
Author(s):  
Thomas J. Hirasuna ◽  
Michael L. Shuler ◽  
Vincent K. Lackney ◽  
Roger M. Spanswick
Keyword(s):  
Cell Cultures ◽  
Anthocyanin Production ◽  
Grape Cell

Immune Electron Microscopy (IEM) of a Canine Adeno-Associated Virus

1974 ◽  
Vol 32 ◽  
pp. 256-257
Author(s):  
Gunter F. Thomas ◽  
M. David Hoggan
Keyword(s):  
Electron Microscopy ◽  
Cell Cultures ◽  
Complement Fixation ◽  
Hepatitis Virus ◽  
Wide Distribution ◽  
Immune Electron Microscopy ◽  
Adeno Associated Virus ◽  
Virus Like Particle ◽  
Dog Kidney ◽  
Green Monkeys

In 1968, Sugimura and Yanagawa described a small 25 nm virus like particle in association with the Matsuda strain of infectious canine hepatitis virus (ICHV). Domoto and Yanagawa showed that this particle was dependent on ICHV for its replication in primary dog kidney cell cultures (PDK) and was resistant to heating at 70°C for 10 min, and concluded that it was a canine adeno-associated virus (CAAV). Later studies by Onuma and Yanagawa compared CAAV with the known human serotypes (AAV 1, 2, 3) and AAV-4, known to be associated with African Green Monkeys. Using the complement fixation (CF) test, they found that CAAV was serologically related to AAV-3 and had wide distribution in the dog population of Japan.

Bacteriophages Associated with Turkey Coecums in Bluecomb-Infected and Healthy Birds

1969 ◽  
Vol 27 ◽  
pp. 226-227
Author(s):  
A. E. Ritchie
Keyword(s):  
Host Cell ◽  
Causal Relationship ◽  
Cell Cultures ◽  
Cell Interaction ◽  
Etiologic Agent ◽  
Viral Origin ◽  
Intestinal Contents ◽  
Host Cell Interaction

The cause of bluecomb disease in turkeys is unknown. Filtration of infective intestinal contents suggests a viral origin. To date, it has not been possible to isolate the etiologic agent in various cell cultures. The purpose of this work was to characterize as many virus-like entities as were recognizable in intestines of both healthy and bluecomb-infected turkeys. By a comparison of the viral populations it was hoped that some insight might be gained into the cause of this disease. Studies of turkey hemorraghic enteritis by Gross and Moore (Avian Dis. 11: 296-307, 1967) have suggested that a bacteriophage-host cell interaction may bear some causal relationship to that disease.

The Fine Structure of Rat Granulosa Cell Cultures Correlated with Progestin Secretion

1973 ◽  
Vol 31 ◽  
pp. 552-553
Author(s):  
T. M. Crisp ◽  
F.R. Denys
Keyword(s):  
Fine Structure ◽  
Granulosa Cell ◽  
Cell Cultures ◽  
Single Injection ◽  
Surrounding Medium ◽  
Petri Dish ◽  
Female Rats ◽  
Vaginal Smear ◽  
Immature Female ◽  
Serum Gonadotropin

The purpose of this paper is to present observations on the fine structure of rat granulosa cell cultures grown in the presence of an adenohypophyseal explant and to correlate the morphology of these cells with progestin secretion. Twenty-six day old immature female rats were given a single injection of 5 IU pregnant mares serum gonadotropin (PMS) in order to obtain ovaries with large vesicular follicles. At 66 hrs. post-PMS administration (estrus indicated by vaginal smear cytology), the ovaries were removed and placed in a petri dish containing medium 199 and 100 U penicillin/streptomycin (P/S)/ml. Under a 20X magnification dissecting microscope, some 5-8 vesicular follicles/ovary were punctured and the granulosa cells were expressed into the surrounding medium. The cells were transferred to centrifuge tubes and spun down at 1000 rpm for 5 mins.

Morphological Examination of a Herpes-type Virus Indigenous for Tree Shrews

1970 ◽  
Vol 28 ◽  
pp. 160-161
Author(s):  
J. P. Brunschwig ◽  
R. M. McCombs ◽  
R. Mirkovic ◽  
M. Benyesh-Melnick
Keyword(s):  
Electron Microscopy ◽  
Soft Tissue ◽  
Chick Embryo ◽  
Soft Tissue Sarcoma ◽  
Cell Cultures ◽  
Tree Shrew ◽  
Type Virus ◽  
Morphological Examination ◽  
Tree Shrews ◽  
Embryo Cells

A new virus, established as a member of the herpesvirus group by electron microscopy, was isolated from spontaneously degenerating cell cultures derived from the kidneys and lungs of two normal tree shrews. The virus was found to replicate best in cells derived from the homologous species. The cells used were a tree shrew cell line, T-23, which was derived from a spontaneous soft tissue sarcoma. The virus did not multiply or did so poorly for a limited number of passages in human, monkey, rodent, rabbit or chick embryo cells. In the T-23 cells, the virus behaved as members of the subgroup B of herpesvirus, in that the virus remained primarily cell associated.

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