Comparative study on responses of cattle and water buffalo (Bubalus bubalis) to experimental inoculation of Brucella abortus biovar 1 by the intraconjunctival route—a preliminary report

ABSTRACT We tested 413 water buffalo cows (142 cases and 271 controls) for the presence of anti-Brucella abortus antibodies (by the skin test, the agglutination test, and the complement fixation test) and the Nramp1 genotype (by capillary electrophoresis). Four alleles (Nramp1A, -B, -C, and -D) were detected in the 3′ untranslated region of the Nramp1 gene. The BB genotype was represented among only controls, providing evidence that this genotype confers resistance to Brucella abortus. The monocytes from the BB (resistant) subjects displayed a higher basal level of Nramp1 mRNA and a lower number of viable intracellular bacteria than did the monocytes from AA (susceptible) subjects. The higher basal level of the antibacterial protein Nramp1 most probably provides the BB animals with the possibility of controlling bacteria immediately after their entry inside the cell.

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Evaluation of cell-mediated immune responses and bacterial clearance in 6–10 months old water buffalo (Bubalus bubalis) experimentally vaccinated with four dosages of commercial Brucella abortus strain RB51 vaccine

Veterinary Immunology and Immunopathology ◽

10.1016/j.vetimm.2005.02.023 ◽

2005 ◽

Vol 106 (3-4) ◽

pp. 209-220 ◽

Cited By ~ 5

Author(s):

M.D. Diptee ◽

A.A. Adesiyun ◽

Z. Asgarali ◽

M. Campbell ◽

G.T. Fosgate

Keyword(s):

Immune Responses ◽

Brucella Abortus ◽

Water Buffalo ◽

Bubalus Bubalis ◽

Bacterial Clearance

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Evaluation of a fluorescence polarization assay for the detection of serum antibodies to Brucella abortus in water buffalo (Bubalus bubalis)

Veterinary Immunology and Immunopathology ◽

10.1016/j.vetimm.2008.05.017 ◽

2008 ◽

Vol 125 (1-2) ◽

pp. 135-142 ◽

Cited By ~ 7

Author(s):

S. Montagnaro ◽

M. Longo ◽

K. Mallardo ◽

G. Pisanelli ◽

L. De Martino ◽

...

Keyword(s):

Brucella Abortus ◽

Fluorescence Polarization ◽

Water Buffalo ◽

Bubalus Bubalis ◽

Serum Antibodies ◽

Fluorescence Polarization Assay

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Genome Sequences of 11 Brucella abortus Isolates from Persistently Infected Italian Regions: TABLE 1

Genome Announcements ◽

10.1128/genomea.01402-15 ◽

2015 ◽

Vol 3 (6) ◽

Cited By ~ 6

Author(s):

Giuliano Garofolo ◽

Jeffrey T. Foster ◽

Kevin Drees ◽

Katiuscia Zilli ◽

Ilenia Platone ◽

...

Keyword(s):

Brucella Abortus ◽

Water Buffalo ◽

Bos Taurus ◽

Bubalus Bubalis ◽

Health Concern ◽

Bovine Brucellosis ◽

Genome Sequences ◽

Italian Regions ◽

Livestock Health ◽

Developed World

Bovine brucellosis, typically caused by Brucella abortus , has been eradicated from much of the developed world. However, the disease remains prevalent in southern Italy, persisting as a public and livestock health concern. We report here the whole-genome sequences of 11 isolates from cattle ( Bos taurus ) and water buffalo ( Bubalus bubalis ) that are representative of the current genetic diversity of B. abortus lineages circulating in Italy.

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Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americas

Veterinary Microbiology ◽

10.1016/j.vetmic.2014.07.011 ◽

2014 ◽

Vol 173 (1-2) ◽

pp. 172-176 ◽

Cited By ~ 3

Author(s):

Diana Martínez ◽

Carolina Thompson ◽

Graciela Draghi ◽

Vilma Canavesio ◽

Roberto Jacobo ◽

...

Keyword(s):

Brucella Abortus ◽

Water Buffalo ◽

Bubalus Bubalis ◽

First Case

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Genetic Resistance to Brucella abortus in the Water Buffalo (Bubalus bubalis)

Infection and Immunity ◽

10.1128/iai.74.4.2115-2120.2006 ◽

2006 ◽

Vol 74 (4) ◽

pp. 2115-2120 ◽

Cited By ~ 36

Author(s):

Giorgia Borriello ◽

Rosanna Capparelli ◽

Michele Bianco ◽

Domenico Fenizia ◽

Flora Alfano ◽

...

Keyword(s):

Brucella Abortus ◽

Water Buffalo ◽

Denaturing Gradient Gel Electrophoresis ◽

Genetic Resistance ◽

Genetic Selection ◽

Bubalus Bubalis ◽

Prolonged Incubation ◽

Gradient Gel Electrophoresis ◽

Selection For

ABSTRACT Brucellosis is a costly disease of water buffaloes (Bubalus bubalis). Latent infections and prolonged incubation of the pathogen limit the efficacy of programs based on the eradication of infected animals. We exploited genetic selection for disease resistance as an approach to the control of water buffalo brucellosis. We tested 231 water buffalo cows for the presence of anti-Brucella abortus antibodies (by the agglutination and complement fixation tests) and the Nramp1 genotype (by PCR-denaturing gradient gel electrophoresis). When the 231 animals (58 cases and 173 controls) were divided into infected (seropositive) and noninfected (seronegative) groups and the Nramp1 genotypes were compared, the seropositive subjects were 52 out of 167 (31%) in the Nramp1A + (Nramp1AA or Nramp1AB) group and 6 out of 64 (9.4%) in the Nramp1A − (Nramp1BB) group (odds ratio, 4.37; 95% confidence limits, 1.87 to 10.19; χ2, 11.65 for 1 degree of freedom). Monocytes from Nramp1BB subjects displayed significantly (P < 0.01) higher levels of Nramp1 mRNA than Nramp1AA subjects and also a significantly (P < 0.01) higher ability in controlling the intracellular replication of several Brucella species in vitro. Thus, selection for the Nramp1BB genotype can become a valuable tool for the control of water buffalo brucellosis in the areas where the disease is endemic.

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