Alterations of hemogram, serum biochemistry, oxidative/nitrosative balance, and copper/zinc homeostasis in dromedary camels naturally infected with poxvirus

Hypozincemia, with hepatic zinc accumulation at the expense of other organs, occurs in infection, inflammation, and aseptic lung injury. Mechanisms underlying zinc partitioning or its impact on extrahepatic organs are unclear. Here we show that the major zinc-binding protein, metallothionein (MT), is critical for zinc transmigration from lung to liver during hyperoxia and preservation of intrapulmonary zinc during hyperoxia is associated with an injury-resistant phenotype in MT-null mice. Particularly, lung-to-liver zinc ratios decreased in wild-type (WT) and increased significantly in MT-null mice breathing 95% oxygen for 72 h. Compared with female adult WT mice, MT-null mice were significantly protected against hyperoxic lung injury indicated by reduced inflammation and interstitial edema, fewer necrotic changes to distal airway epithelium, and sustained lung function at 72 h hyperoxia. Lungs of MT-null mice showed decreased levels of immunoreactive LC3, an autophagy marker, compared with WT mice. Analysis of superoxide dismutase (SOD) activity in the lungs revealed similar levels of manganese-SOD activity between strains under normoxia and hyperoxia. Lung extracellular SOD activity decreased significantly in both strains at 72 h of hyperoxia, although there was no difference between strains. Copper-zinc-SOD activity was ∼4× higher under normoxic conditions in MT-null compared with WT mice but was not affected in either group by hyperoxia. Collectively the data suggest that genetic deletion of MT-I/II in mice is associated with compensatory increase in copper-zinc-SOD activity, prevention of hyperoxia-induced zinc transmigration from lung to liver, and hyperoxia-resistant phenotype strongly associated with differences in zinc homeostasis during hyperoxic acute lung injury.

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Copper-To-Zinc Ratio as an Inflammatory Marker in Patients with Sickle Cell Disease

Sci ◽

10.3390/sci2040089 ◽

2020 ◽

Vol 2 (4) ◽

pp. 89

Author(s):

Mathias Abiodun Emokpae ◽

Emmanuel Bamidele Fatimehin

Keyword(s):

Sickle Cell Disease ◽

Sickle Cell ◽

Serum Levels ◽

Serum Copper ◽

The Body ◽

Zinc Homeostasis ◽

Clinical State ◽

Cell Disease ◽

Copper And Zinc ◽

Copper Zinc

Sickle cell disease (SCD) is an inherited disorder and a major health challenge in Nigeria. Micronutrient deficiencies often associated with the disorder may cause inflammation and abnormal metabolism in the body. The copper-to-zinc ratio is a more relevant diagnostic measure than the concentration of either metal alone in clinical practice. This study seeks to evaluate serum levels of c-reactive protein (CRP), copper, zinc and the copper-to-zinc ratio, and to correlate the latter with CRP in adult subjects with SCD. Serum copper, zinc, CRP and plasma fibrinogen were assayed in 100 confirmed SCD patients in steady clinical state and 100 age- and sex-matched subjects with normal hemoglobin. Serum copper and zinc were assayed by the colorimetric method using reagents supplied by Centronic, Germany, while CRP and fibrinogen were assayed using reagents supplied by Sigma (St. Louis, MO, USA) and Anogen (Ontario, Canada), respectively. The copper-to-zinc ratio was calculated from serum levels of copper and zinc. The measured parameters were compared between the groups using the Students t-test, and the Pearson correlation coefficient was used to relate CRP with the other parameters. Serum copper, CRP, fibrinogen and the copper-to-zinc ratio were significantly higher (p < 0.001), while zinc level was lower in SCD patients than in controls. Serum CRP concentration correlated with copper (r = 0.10; p < 0.02), zinc (r = −0.199; p < 0.05) and the copper-to-zinc ratio (r = 0.312; p < 0.002), but the correlation between CRP and fibrinogen was not significant. Inflammation may modulate copper and zinc homeostasis, and the copper-to-zinc ratio may be used as a marker of nutritional deficiency and inflammation in SCD patients.

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Efficacy of Microencapsulated Probiotic as Adjunct Therapy on Resolution of Diarrhea, Copper-Zinc Homeostasis, Immunoglobulins, and Inflammatory Markers in Serum of Spontaneous Rotavirus-Infected Diarrhoetic Calves

Probiotics and Antimicrobial Proteins ◽

10.1007/s12602-021-09862-9 ◽

2021 ◽

Author(s):

Jitendra Singh Gandhar ◽

Ujjwal Kumar De ◽

Anju Kala ◽

Yashpal Singh Malik ◽

Supriya Yadav ◽

...

Keyword(s):

Inflammatory Markers ◽

Zinc Homeostasis ◽

Adjunct Therapy ◽

Copper Zinc

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Clioquinol changes the expression profiles and redox states of proteins involved in copper/zinc homeostasis

Proceedings for Annual Meeting of The Japanese Pharmacological Society ◽

10.1254/jpssuppl.93.0_3-p-378 ◽

2020 ◽

Vol 93 (0) ◽

pp. 3-P-378

Author(s):

Masato Katsuyama ◽

Chihiro Yabe-Nishimura

Keyword(s):

Expression Profiles ◽

Zinc Homeostasis ◽

Redox States ◽

Copper Zinc

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Copper-to-Zinc Ratio Correlates with an Inflammatory Marker in Patients with Sickle Cell Disease (Version 1)

Sci ◽

10.3390/sci1020034 ◽

2019 ◽

Vol 1 (2) ◽

pp. 34

Author(s):

Mathias Abiodun Emokpae ◽

Emmanuel Babatunde Fatimehin

Keyword(s):

Sickle Cell Disease ◽

Sickle Cell ◽

Serum Levels ◽

Serum Copper ◽

The Body ◽

Zinc Homeostasis ◽

Clinical State ◽

Cell Disease ◽

Copper And Zinc ◽

Copper Zinc

Sickle cell disease (SCD) is an inherited disorder of major health challenge in Nigeria. Micronutrients deficiencies often associated with the disorder may cause inflammation and abnormal metabolisms in the body. The copper-to-zinc ratio is a more important assessment than the concentrations of either of the metals in clinical practice. This study seeks to evaluate serum levels of c-reactive protein (CRP), copper, zinc and copper-to-zinc ratio and to correlate copper-to-zinc ratio with CRP in adult subjects with SCD. Serum copper, zinc, CRP and plasma fibrinogen were assayed in 100 confirmed SCD patients in steady clinical state and 100 age and sex matched subjects with normal haemoglobin. Serum copper and zinc were assayed by colorimetric method using reagents supplied by Centronic, Germany while CRP and fibrinogen were assayed using reagents supplied by Sigma (St. Louis, MO, USA) and Anogen (Ontario, Canada), respectively. The copper to zinc ratio was calculated from serum levels of copper and zinc. The measured parameters were compared between the groups using Students t-test and Pearson correlation coefficient was used to relate CRP with the other parameters. Serum copper, CRP, fibrinogen and copper-to-zinc ratio were significantly higher (p < 0.001) while zinc level was lower in SCD patients than controls. Serum CRP concentration correlated with copper (r = 0.10; p < 0.02), zinc (r = −0.199; p < 0.05) and Copper-to-zinc ratio (r = 0.312; p < 0.002) but the correlation between CRP and fibrinogen was not significant. Inflammatory condition may modulate copper and zinc homeostasis and copper-to-zinc ratio may be used as marker of nutritional deficiency and inflammation in SCD patients.

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Copper-to-Zinc Ratio Correlates with an Inflammatory Marker in Patients with Sickle Cell Disease

Sci ◽

10.3390/sci1020055 ◽

2019 ◽

Vol 1 (2) ◽

pp. 55

Author(s):

Mathias Abiodun Emokpae ◽

Emmanuel Bamidele Fatimehin

Keyword(s):

Sickle Cell Disease ◽

Sickle Cell ◽

Serum Levels ◽

Serum Copper ◽

The Body ◽

Zinc Homeostasis ◽

Clinical State ◽

Cell Disease ◽

Copper And Zinc ◽

Copper Zinc

Sickle cell disease (SCD) is an inherited disorder of major health challenge in Nigeria. Micronutrients deficiencies often associated with the disorder may cause inflammation and abnormal metabolisms in the body. The copper-to-zinc ratio is a more important assessment than the concentrations of either of the metals in clinical practice. This study seeks to evaluate serum levels of c-reactive protein (CRP), copper, zinc and copper-to-zinc ratio and to correlate copper-to-zinc ratio with CRP in adult subjects with SCD. Serum copper, zinc, CRP and plasma fibrinogen were assayed in 100 confirmed SCD patients in steady clinical state and 100 age and sex matched subjects with normal haemoglobin. Serum copper and zinc were assayed by colorimetric method using reagents supplied by Centronic, Germany while CRP and fibrinogen were assayed using reagents supplied by Sigma (St. Louis, MO, USA) and Anogen (Ontario, Canada), respectively. The copper to zinc ratio was calculated from serum levels of copper and zinc. The measured parameters were compared between the groups using Students t-test and Pearson correlation coefficient was used to relate CRP with the other parameters. Serum copper, CRP, fibrinogen and copper-to-zinc ratio were significantly higher (p < 0.001) while zinc level was lower in SCD patients than controls. Serum CRP concentration correlated with copper (r = 0.10; p < 0.02), zinc (r = −0.199; p < 0.05) and Copper-to-zinc ratio (r = 0.312; p < 0.002) but the correlation between CRP and fibrinogen was not significant. Inflammatory condition may modulate copper and zinc homeostasis and copper-to-zinc ratio may be used as marker of nutritional deficiency and inflammation in SCD patients.

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Concentration of selected micro-nutrients in saliva among women in menopausal period treated with hormone replacement therapy

Osteologie/Osteology ◽

10.1055/s-0038-1630097 ◽

2013 ◽

Vol 22 (01) ◽

pp. 46-51

Author(s):

M. Rahnama ◽

I. Jastrzêbska-Jamrogiewicz ◽

R. Jamrogiewicz

Keyword(s):

Bone Mineral Density ◽

Hormone Replacement Therapy ◽

Blood Serum ◽

Replacement Therapy ◽

Hormone Replacement ◽

Surgical Removal ◽

Femoral Bone ◽

Mineral Density ◽

Natural Menopause ◽

Copper Zinc

Summary Objective: The aim of this study was to observe the variability of the level of copper, zinc and manganese in saliva amongst women with hypoestrogenia, treated and untreated with hormone replacement therapy (HRT). Methods: The study was conducted on a group of 60 women treated and untreated with HRT. Half of the patients were after natural menopause and other half was after surgical removal of ovaries. Research on micronutrients in non-stimulated saliva and blood serum was carried out in 2005. Tests on saliva were repeated in 2010. Investigation of bone mineral density (BMD) of femoral bone was performed in year 2010. Results: Statistical analysis of concentration of copper and zinc revealed a linear correlation between the levels of these microelements in blood serum and saliva. The study revealed that HRT has a beneficial effect on BMD and the concentration of copper and manganese in saliva and blood serum of patients after the menopause. Patients treated with HRT showed higher BMD values than groups not treated with hormones. Conclusions: Saliva appears to be a promising diagnostic material which can be used to analyze the content of trace elements, but further research should be carried out on a broader research group.

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