Experimental Study on the Effect of the Physicochemical Properties of Contaminated Fillers in Bioretention System on Microbial Community Structure

The continuous cropping of plants can result in the disruption of the soil microbial community and caused significant declines in yields. However, there are few reports on the effects of continuous cropping of sugarcane on the microbial community structure and functional pathway. In the current study, we analyzed the structural and functional changes of microbial community structure in the rhizospheric soil of sugarcane in different continuous cropping years using Illumina Miseq high-throughput sequencing and metagenomics analysis. We collected rhizosphere soils from fields of no continuous cropping history (NCC), 10 years of continuous cropping (CC10), and 30 years of continuous cropping (CC30) periods in the Fujian province. The results demonstrated that continuous sugarcane cropping resulted in significant changes in the physicochemical properties of soil and the composition of soil bacterial and fungal communities. With the continuous cropping, the crop yield dramatically declined from NCC to CC30. Besides, the redundancy analysis (RDA) of the dominant bacterial and fungal phyla and soil physicochemical properties revealed that the structures of the bacterial and fungal communities were mainly driven by pH and TS. Analysis of potential functional pathways during the continuous cropping suggests that different KEGG pathways were enriched in different continuous cropping periods. The significant reduction of bacteria associated with rhizospheric soil nitrogen and sulfur cycling functions and enrichment of pathogenic bacteria may be responsible for the reduction of effective nitrogen and total sulfur content in rhizospheric soil of continuous sugarcane as well as the reduction of sugarcane yield and sugar content. Additionally, genes related to nitrogen and sulfur cycling were identified in our study, and the decreased abundance of nitrogen translocation genes and AprAB and DsrAB in the dissimilatory sulfate reduction pathway could be the cause of declined biomass. The findings of this study may provide a theoretical basis for uncovering the mechanism of obstacles in continuous sugarcane cropping and provide better guidance for sustainable development of the sugarcane.

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Microbial community structure and relationship with physicochemical properties of soil stockpiles in selected South African opencast coal mines

Soil Science & Plant Nutrition ◽

10.1080/00380768.2019.1621667 ◽

2019 ◽

Vol 65 (4) ◽

pp. 332-341 ◽

Cited By ~ 5

Author(s):

Obinna T. Ezeokoli ◽

Sannie K. Mashigo ◽

D. Garry Paterson ◽

Cornelius C. Bezuidenhout ◽

Rasheed A. Adeleke

Keyword(s):

Community Structure ◽

Microbial Community ◽

Physicochemical Properties ◽

South African ◽

Microbial Community Structure ◽

Coal Mines ◽

Opencast Coal Mines ◽

Properties Of Soil

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Changes in the Physicochemical Properties and Microbial Communities of Rhizospheric Soil after Cassava/Peanut Intercropping

10.1101/570937 ◽

2019 ◽

Author(s):

Xiumei Tang ◽

Saiyun Luo ◽

Zhipeng Huang ◽

Haining Wu ◽

Jin Wang ◽

...

Keyword(s):

Community Structure ◽

Microbial Community ◽

16S Rrna ◽

Physicochemical Properties ◽

Microbial Community Structure ◽

Enzyme Activities ◽

Southern China ◽

Nutrient Content ◽

Rrna Gene ◽

Rhizospheric Soil

ABSTRACTCassava/peanut intercropping is a popular cultivation method in southern China and has the advantages of apparently increased yield and economic efficiency compared with monoculture, however, the ecological benefits of this method are poorly understood. This study aimed to investigate the effects of intercropping on the physicochemical properties and microbial community structures of soil. Field trials were performed to determine the effects of cassava/peanut intercropping on rhizospheric soil nutrient content, enzyme activities, microbial quantity and microbial community structure. The microbial community was characterized by 16S rRNA tag-based high-throughput sequencing on the Illumina MiSeq platform. Results showed that cassava/peanut intercropping could improve the physicochemical properties of rhizospheric soil by increasing the available nutrient content, pH, bacterial quantity, and some enzyme activities and by altering the microbial community structure. 16S rRNA gene sequencing demonstrated that the microbial community structure varied between the intercropping and monoculture systems. Nitrospirae, Verrucomicrobia and Gemmatimonadetes were more abundant in the intercropping system than in the monocultures. Redundancy analysis (RDA) revealed that the abundances ofDA101,PilimeliaandRamlibacterwere positively correlated with environmental parameters such as available nitrogen and pH, and these were dominant genera in the rhizospheric soil of the intercropped peanut plants.

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