Recombinant Escherichia coli heat-labile enterotoxin B subunit humoral adjuvant effect depends on dose and administration route

This study aimed to investigate the adjuvant effect of recombinant attenuatedSalmonellaexpressing cholera toxin B subunit (CTB) andEscherichia coliheat-labile enterotoxin B subunit (LTB) for the P-fimbriae subunit-based vaccine of avian pathogenicE. coli(APEC) in a murine model. The PapA-specific sIgA and IgG responses were significantly enhanced after immunisation with theSalmonella-PapA vaccine in the presence of CTB or LTB. The group immunised with theSalmonella-LTB strain promoted Th1-type immunity, whereas that immunised with theSalmonella-CTB strain produced Th2-type immunity. We concluded that bothSalmonella-CTB and -LTB strains can enhance the immune response to PapA, and that the LTB strain may be a more effective adjuvant for APEC vaccination, which requires higher Th1-type immunity for protection. Thus, our findings provide evidence that immunisation with an adjuvant, LTB, is one of the strategies of developing effective vaccines against P-fimbriated APEC.

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Efficient extracellular production of recombinant Escherichia coli heat-labile enterotoxin B subunit by using the expression/secretion system of Bacillus brevis and its mucosal immunoadjuvanticity

Vaccine ◽

10.1016/s0264-410x(99)00547-2 ◽

2000 ◽

Vol 18 (17) ◽

pp. 1730-1737 ◽

Cited By ~ 9

Author(s):

Satoshi Kozuka ◽

Yoko Yasuda ◽

Masanori Isaka ◽

Nobutake Masaki ◽

Tooru Taniguchi ◽

...

Keyword(s):

Escherichia Coli ◽

Secretion System ◽

Recombinant Escherichia Coli ◽

Extracellular Production ◽

Bacillus Brevis ◽

B Subunit ◽

Heat Labile ◽

Enterotoxin B

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Attenuated Salmonella Gallinarum secreting an Escherichia coli heat-labile enterotoxin B subunit protein as an adjuvant for oral vaccination against fowl typhoid

Veterinary Immunology and Immunopathology ◽

10.1016/j.vetimm.2012.09.006 ◽

2012 ◽

Vol 150 (3-4) ◽

pp. 149-160 ◽

Cited By ~ 9

Author(s):

Byung Woo Jeon ◽

Chetan V. Jawale ◽

Seung Hwan Kim ◽

John Hwa Lee

Keyword(s):

Escherichia Coli ◽

Oral Vaccination ◽

Salmonella Gallinarum ◽

Subunit Protein ◽

B Subunit ◽

Heat Labile ◽

Fowl Typhoid ◽

Enterotoxin B

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Tissue culture and expression of Escherichia coli heat-labile enterotoxin B subunit in transgenic Peperomia pellucida

Protein Expression and Purification ◽

10.1016/j.pep.2010.02.010 ◽

2010 ◽

Vol 72 (1) ◽

pp. 82-86 ◽

Cited By ~ 11

Author(s):

Nguyen Hoang Loc ◽

Nguyen Hoang Bach ◽

Tae-Geum Kim ◽

Moon-Sik Yang

Keyword(s):

Escherichia Coli ◽

Tissue Culture ◽

B Subunit ◽

Heat Labile ◽

Enterotoxin B

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Escherichia coli Heat-Labile Enterotoxin B Subunit Is a More Potent Mucosal Adjuvant than Its Closely Related Homologue, the B Subunit of Cholera Toxin

Infection and Immunity ◽

10.1128/iai.69.5.3476-3482.2001 ◽

2001 ◽

Vol 69 (5) ◽

pp. 3476-3482 ◽

Cited By ~ 57

Author(s):

Douglas G. Millar ◽

Timothy R. Hirst ◽

Denis P. Snider

Keyword(s):

Escherichia Coli ◽

Cholera Toxin ◽

Lymphocyte Proliferation ◽

Vaccine Adjuvants ◽

B Subunit ◽

Heat Labile ◽

Antibody Titers ◽

Enterotoxin B ◽

Draining Lymph Nodes ◽

Stimulation Of

ABSTRACT Although cholera toxin (Ctx) and Escherichia coliheat-labile enterotoxin (Etx) are known to be potent mucosal adjuvants, it remains controversial whether the adjuvanticity of the holotoxins extends to their nontoxic, receptor-binding B subunits. Here, we have systematically evaluated the comparative adjuvant properties of highly purified recombinant EtxB and CtxB. EtxB was found to be a more potent adjuvant than CtxB, stimulating responses to hen egg lysozyme when the two were coadministered to mice intranasally, as assessed by enhanced serum and secretory antibody titers as well as by stimulation of lymphocyte proliferation in spleen and draining lymph nodes. These results indicate that, although structurally very similar, EtxB and CtxB have strikingly different immunostimulatory properties and should not be considered equivalent as prospective vaccine adjuvants.

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