Secretory Expression and Purification of Recombinant Escherichia coli Heat-Labile Enterotoxin B Subunit and its Applications on Intranasal Vaccination of Hantavirus

2008 ◽  
Vol 41 (2) ◽  
pp. 91-98 ◽  
Author(s):  
Shouchun Cao ◽  
Ying Zhang ◽  
Feng Liu ◽  
Qin Wang ◽  
Quanfu Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli ◽  
Expression And Purification ◽  
Secretory Expression ◽  
B Subunit ◽  
Heat Labile ◽  
Enterotoxin B

Recombinant Escherichia coli heat-labile enterotoxin B subunit humoral adjuvant effect depends on dose and administration route

2009 ◽  
Vol 26 (3) ◽  
pp. 489-495 ◽  
Author(s):  
Geferson Fischer ◽  
Fabricio R. Conceição ◽  
Fábio P. L. Leite ◽  
Carina M. Moraes ◽  
Lílian N. Ferreira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli ◽  
Administration Route ◽  
Adjuvant Effect ◽  
B Subunit ◽  
Heat Labile ◽  
Enterotoxin B

Tissue culture and expression of Escherichia coli heat-labile enterotoxin B subunit in transgenic Peperomia pellucida

2010 ◽  
Vol 72 (1) ◽  
pp. 82-86 ◽  
Author(s):  
Nguyen Hoang Loc ◽  
Nguyen Hoang Bach ◽  
Tae-Geum Kim ◽  
Moon-Sik Yang
Keyword(s):  
Escherichia Coli ◽  
Tissue Culture ◽  
B Subunit ◽  
Heat Labile ◽  
Enterotoxin B

scholarly journals Escherichia coli Heat-Labile Enterotoxin B Subunit Is a More Potent Mucosal Adjuvant than Its Closely Related Homologue, the B Subunit of Cholera Toxin

2001 ◽  
Vol 69 (5) ◽  
pp. 3476-3482 ◽  
Author(s):  
Douglas G. Millar ◽  
Timothy R. Hirst ◽  
Denis P. Snider
Keyword(s):  
Escherichia Coli ◽  
Cholera Toxin ◽  
Lymphocyte Proliferation ◽  
Vaccine Adjuvants ◽  
B Subunit ◽  
Heat Labile ◽  
Antibody Titers ◽  
Enterotoxin B ◽  
Draining Lymph Nodes ◽  
Stimulation Of

ABSTRACT Although cholera toxin (Ctx) and Escherichia coliheat-labile enterotoxin (Etx) are known to be potent mucosal adjuvants, it remains controversial whether the adjuvanticity of the holotoxins extends to their nontoxic, receptor-binding B subunits. Here, we have systematically evaluated the comparative adjuvant properties of highly purified recombinant EtxB and CtxB. EtxB was found to be a more potent adjuvant than CtxB, stimulating responses to hen egg lysozyme when the two were coadministered to mice intranasally, as assessed by enhanced serum and secretory antibody titers as well as by stimulation of lymphocyte proliferation in spleen and draining lymph nodes. These results indicate that, although structurally very similar, EtxB and CtxB have strikingly different immunostimulatory properties and should not be considered equivalent as prospective vaccine adjuvants.

Mass production of somatic embryos expressing Escherichia coli heat-labile enterotoxin B subunit in Siberian ginseng

2006 ◽  
Vol 121 (2) ◽  
pp. 124-133 ◽  
Author(s):  
Tae-Jin Kang ◽  
Won-Seok Lee ◽  
Eun-Gyung Choi ◽  
Jae-Whune Kim ◽  
Bang-Geul Kim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Mass Production ◽  
Somatic Embryos ◽  
B Subunit ◽  
Siberian Ginseng ◽  
Heat Labile ◽  
Enterotoxin B
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