Ecotoxicological study of six drugs in Aliivibrio fischeri, Daphnia magna and Raphidocelis subcapitata

Aromatic amines (anilines and related derivates) are an important class of environmental pollutants that can be released to the aquatic environment as industrial effluents or as breakdown products of pesticides and dyes. The toxicities of aniline, 2-chloroaniline, 3-chloroaniline, 4-chloroaniline and 3,5-dichloroaniline towards a multitrophic test battery comprised of bacteria Aliivibrio fischeri (formerly Vibrio fischeri), a ciliated protozoan Tetrahymena thermophila and two crustaceans (Daphnia magna and Thamnocephalus platyurus) were investigated. Under the applied test conditions, the toxicities of the anilines notably varied among the test species. The bacteria and protozoa were much less sensitive towards the anilines than the crustaceans: EC50 values 13-403 mg L-1 versus 0.13-15.2 mg L-1. No general tendency between toxicity and the chemical structure of the anilines (the degree of chloro-substitution and the position of the chloro-substituents) was found in the case of all the tested aquatic species. The replacement of the artificial test medium (ATM) by the river water remarkably decreased the toxicity of anilines to crustaceans but not to protozoa. This research is part of the EU 6th Framework Integrated Project OSIRIS, in which ecotoxicogenomic studies of anilines (e.g., for Daphnia magna) will also be performed that may help to clarify the mechanisms of toxicity of different anilines.

Download Full-text

Aquatic Toxicity Comparison of Silver Nanoparticles and Silver Nanowires

BioMed Research International ◽

10.1155/2015/893049 ◽

2015 ◽

Vol 2015 ◽

pp. 1-12 ◽

Cited By ~ 17

Author(s):

Eun Kyung Sohn ◽

Seyed Ali Johari ◽

Tae Gyu Kim ◽

Jin Kwon Kim ◽

Ellen Kim ◽

...

Keyword(s):

Silver Nanoparticles ◽

Daphnia Magna ◽

Silver Nanowires ◽

Aquatic Toxicity ◽

Oryzias Latipes ◽

Aquatic Environments ◽

Silver Nanomaterials ◽

Raphidocelis Subcapitata ◽

Test Organisms ◽

Test Fish

To better understand the potential ecotoxicological impact of silver nanoparticles (AgNPs) and silver nanowires (AgNWs) released into freshwater environments, the toxicities of these nanomaterials were assessed and compared using Organization for Economic Cooperation and Development (OECD) test guidelines, including a “Daphniasp., acute immobilization test,” “Fish, acute toxicity test,” and “freshwater alga and cyanobacteria, growth inhibition test.” Based on the estimated median lethal/effective concentrations of AgNPs and AgNWs, the susceptibility to the nanomaterials was different among test organisms (daphnia > algae > fish), suggesting that the AgNPs are classified as “category acute 1” forDaphnia magna, “category acute 2” forOryzias latipes, and “category acute 1” forRaphidocelis subcapitata, while the AgNWs are classified as “category acute 1” forDaphnia magna, “category acute 2” forOryzias latipes, and “category acute 2” forRaphidocelis subcapitata, according to the GHS (Globally Harmonized System of Classification and Labelling of Chemicals). In conclusion, the present results suggest that more attention should be paid to prevent the accidental or intentional release of silver nanomaterials into freshwater aquatic environments.

Download Full-text

Toxicity prediction and assessment of an environmentally realistic pesticide mixture to Daphnia magna and Raphidocelis subcapitata

Ecotoxicology ◽

10.1007/s10646-018-1938-0 ◽

2018 ◽

Vol 27 (7) ◽

pp. 956-967 ◽

Cited By ~ 7

Author(s):

E. Silva ◽

C. Martins ◽

A. S. Pereira ◽

S. Loureiro ◽

M. J. Cerejeira

Keyword(s):

Daphnia Magna ◽

Toxicity Prediction ◽

Pesticide Mixture ◽

Raphidocelis Subcapitata

Download Full-text

Aquatic toxicity of several textile dye formulations: Acute and chronic assays with Daphnia magna and Raphidocelis subcapitata

Ecotoxicology and Environmental Safety ◽

10.1016/j.ecoenv.2017.05.046 ◽

2017 ◽

Vol 144 ◽

pp. 79-87 ◽

Cited By ~ 26

Author(s):

Roberta Croce ◽

Filippo Cinà ◽

Anna Lombardo ◽

Gregory Crispeyn ◽

Claudia Ileana Cappelli ◽

...

Keyword(s):

Daphnia Magna ◽

Aquatic Toxicity ◽

Textile Dye ◽

Raphidocelis Subcapitata

Download Full-text

Correlation between acute toxicity for Daphnia magna, Aliivibrio fischeri and physicochemical variables of the leachate produced in landfill simulator reactors

Environmental Technology ◽

10.1080/09593330.2017.1281352 ◽

2017 ◽

Vol 38 (22) ◽

pp. 2898-2906 ◽

Cited By ~ 2

Author(s):

José J. Barrios Restrepo ◽

Letícia Flohr ◽

Silvia P. Melegari ◽

Cristina H. da Costa ◽

Cristiane F. Fuzinatto ◽

...

Keyword(s):

Acute Toxicity ◽

Daphnia Magna ◽

Physicochemical Variables ◽

Aliivibrio Fischeri

Download Full-text

Sensitivity of Zebrafish (Danio rerio) Embryos to Hospital Effluent Compared to Daphnia magna and Aliivibrio fischeri

Physiological Research ◽

10.33549/physiolres.934616 ◽

2020 ◽

pp. S681-S691

Author(s):

M WITTLEROVÁ ◽

G JÍROVÁ ◽

A VLKOVÁ ◽

K KEJLOVÁ ◽

M MALÝ ◽

...

Keyword(s):

Acute Toxicity ◽

Daphnia Magna ◽

Sublethal Effects ◽

Aquatic Organisms ◽

Spinal Curvature ◽

Treated Wastewater ◽

Zebrafish Embryos ◽

Fish Embryo ◽

Additional Tool ◽

Aliivibrio Fischeri

The Fish Embryo Acute Toxicity (FET) Test was adopted by the Organisation for Economic Co-operation and Development as OECD TG 236 in 2013. The test has been designed to determine acute toxicity of chemicals on embryonic stages of fish and proposed as an alternative method to the Fish Acute Toxicity Test performed according to OECD TG 203. In recent years fish embryos were used not only in the assessment of toxicity of chemicals but also for environmental and wastewater samples. In our study we investigated the acute toxicity of treated wastewater from seven hospitals in the Czech Republic. Our main purpose was to compare the suitability and sensitivity of zebrafish embryos with the sensitivity of two other aquatic organisms commonly used for wastewater testing – Daphnia magna and Aliivibrio fischeri. For the aim of this study, in addition to the lethal endpoints of the FET test, sublethal effects such as delayed heartbeat, lack of blood circulation, pericardial and yolk sac edema, spinal curvature and pigmentation failures were evaluated. The comparison of three species demonstrated that the sensitivity of zebrafish embryos is comparable or in some cases higher than the sensitivity of D. magna and A. fischeri. The inclusion of sublethal endpoints caused statistically significant increase of the FET test efficiency in the range of 1-12 %. Based on our results, the FET test, especially with the addition of sublethal effects evaluation, can be considered as a sufficiently sensitive and useful additional tool for ecotoxicity testing of the acute toxicity potential of hospital effluents.

Download Full-text

Mixture toxicity of six pharmaceuticals towards Aliivibrio fischeri, Daphnia magna, and Lemna minor

Environmental Science and Pollution Research ◽

10.1007/s11356-021-17928-y ◽

2021 ◽

Author(s):

Anna Białk-Bielińska ◽

Łukasz Grabarczyk ◽

Ewa Mulkiewicz ◽

Alan Puckowski ◽

Stefan Stolte ◽

...

Keyword(s):

Daphnia Magna ◽

Lemna Minor ◽

Opioid Analgesic ◽

Mixture Toxicity ◽

Concentration Addition ◽

Case Scenario ◽

Worst Case ◽

Ca Model ◽

Inflammatory Drugs ◽

Aliivibrio Fischeri

AbstractAs the knowledge on the joint effects of pharmaceuticals towards different non-target organisms is still limited, the aim of our study was to evaluate the toxicity of mixtures of pharmaceuticals, as well as their baseline toxicity towards three selected organisms, namely the bioluminescent bacteria Aliivibrio fischeri, the crustacean Daphnia magna, and the duckweed Lemna minor. Different mixtures composed of three up to five pharmaceuticals having the same or different mechanisms of action in terms of their therapeutic activity (non-steroidal anti-inflammatory drugs, opioid analgesic, antibacterial and anti-epileptic drugs) were investigated. The observed EC50s were compared with those predicted using the concentration addition (CA) and independent action (IA) models. In general, the EC50 values for mixtures predicted with the CA model were lower than those obtained with the IA model, although, in some cases, test predictions of these two models were almost identical. Most of the experimentally determined EC50 values for the specific mixtures were slightly higher than those predicted with the CA model; hence, a less than additive effect was noted. Based on the obtained results, it might be concluded that the CA model assumes the worst-case scenario and gives overall closer predictions; therefore, it should be recommended also for modeling the mixture toxicity of pharmaceuticals with different modes of action.

Download Full-text

Ecotoxic Effects of the Vehicle Solvent Dimethyl Sulfoxide on Aquatic Model Organisms

10.21203/rs.3.rs-1069241/v1 ◽

2021 ◽

Author(s):

Larissa Andrade-Vieira ◽

Clement Bojic ◽

Ingrid Alvarenga ◽

Teotonio de Carvalho ◽

Jean-François Masfaraud ◽

...

Keyword(s):

Growth Rate ◽

Population Growth ◽

Dimethyl Sulfoxide ◽

Daphnia Magna ◽

Population Growth Rate ◽

Exposure Period ◽

Effective Concentration ◽

Model Organisms ◽

Raphidocelis Subcapitata ◽

Frequent Use

Abstract Dimethyl sulfoxide (DMSO) is widely used as a vehicle solvent in ecotoxicity bioassays. However, despite its frequent use, itcould be toxic for organisms at some concentrations. Hence, the aim of this study was to investigate the effectsof DMSO on the population growth rate of the microalgaeRaphidocelis subcapitata, the mobility of the microcrustacean Daphnia magna,and the reproduction of the rotiferBrachionus calyciflorus. DMSO was applied to the organisms in concentrations ranging from 0.031–4%. For R. subcapitata significant effects in growth inhibitionafter 72 h of exposure was 0.125% DMSO,being the lowest observed effectconcentration (LOEC). The 50% effective concentration (EC50) was2.138 ± 0.372%. In D. magna,significant differences in the mobility after 24 h or 48 h of exposure was 1% DMSO being 1.712± 0.207% and 1.167± 0.220%DMSO the EC50 observed for 24 h and 48 h exposure, respectively. For B. calyciflorus,it was not possible to validate the tests performed, as there were insufficient animals alive in the control conditions at the end of the exposure period. Therefore, we recommended avoiding DMSO as a vehicle in assays using B. calyciflorus,and to use final DMSO concentrationsin experimental solution not exceeding 0.125% forR. subcapitata and 0.5% for D. magna.

Download Full-text