scholarly journals Simultaneous high-efficiency removal of sulfamethoxazole and zinc (II) from livestock and poultry breeding wastewater by a novel dual-functional bacterium, Bacillus sp. SDB4

2021 ◽  
Author(s):  
Xi Chen ◽  
Hai Lin ◽  
Yingbo Dong ◽  
Bing Li ◽  
Tingting Yin ◽  
...  
Keyword(s):  
High Efficiency ◽  
Bacillus Sp ◽  
Dual Functional ◽  
Poultry Breeding ◽  
Bacterium Bacillus

scholarly journals Simultaneous High-Efficiency Removal of Sulfamethoxazole and zinc (II) From Livestock and Poultry Breeding Wastewater by a Novel Dual-Functional Bacterium, Bacillus sp. SDB4

2021 ◽  
Author(s):  
Xi Chen ◽  
Hai Lin ◽  
Yingbo Dong ◽  
Bing Li ◽  
Tingting Yin ◽  
...  
Keyword(s):  
Heavy Metals ◽  
High Efficiency ◽  
Transformation Product ◽  
Dual Functional ◽  
Biological Removal ◽  
Low Concentrations ◽  
Poultry Breeding ◽  
Bacterium Bacillus ◽  
Maximum Removal ◽  
Functional Strain

Abstract The complex mixtures of antibiotics and heavy metals are commonly existed in livestock and poultry breeding wastewater. Effective and simultaneous removal of these toxic compounds by microorganisms, especially single strains remains a considerable challenge. In this study, a novel functional strain SDB4, isolated from duck manure and identified as Bacillus sp., has been shown to possess high removal abilities for both sulfamethoxazole (SMX) and Zn2+. The maximum removal efficiency achieved 73.97% for SMX and 84.06% for Zn2+ within 48 h in sole system. It has great potential for eliminating SMX along with Zn2+, 78.45% of SMX and 52.91% of Zn2+ were removed in the 20 mg·L-1 SMX and 100 mg·L-1 Zn2+ binary system. Furthermore, the SMX-biotransformation ability of SDB4 was enhanced at low concentrations of Zn2+ (below 100 mg·L-1). N4-acetyl-SMX was identified as the main stable transformation product during SMX bioremoval. FTIR analyses revealed that OH, NH2, C=O, C-N/N-H and C-O-C played major roles in the biosorption of Zn2+. Our study of the dually functioning strain SDB4 provides a potential application for the simultaneous biological removal of antibiotics and heavy metals.

A primary study on nattokinase of bacterium Bacillus sp. isolated from nem chua

2015 ◽  
Vol 37 (1se) ◽  
Author(s):  
Nguyen Quynh Uyen ◽  
Hoang Thu Ha ◽  
Nguyen Hong Nhung ◽  
Phan Thi Ha ◽  
Nguyen Huynh Minh Quyen
Keyword(s):  
Primary Study ◽  
Bacillus Sp ◽  
Bacterium Bacillus

scholarly journals Purification and Characterization of a Novel Alginate Lyase from the Marine Bacterium Bacillus sp. Alg07

Marine Drugs ◽  
2018 ◽  
Vol 16 (3) ◽  
pp. 86 ◽  
Author(s):  
Peng Chen ◽  
Yueming Zhu ◽  
Yan Men ◽  
Yan Zeng ◽  
Yuanxia Sun
Keyword(s):  
Marine Bacterium ◽  
Alginate Lyase ◽  
Bacillus Sp ◽  
Purification And Characterization ◽  
Bacterium Bacillus

scholarly journals Overexpression of Thermophilic α-amylase in Bacillus Licheniformis using a High Efficiency Chromosomal Integration and Amplification Strategy

2021 ◽  
Author(s):  
Peili Shen ◽  
Dandan Niu ◽  
Xuelian Liu ◽  
Kangming Tian ◽  
Permaul Kugenthiren ◽  
...  
Keyword(s):  
Bacillus Licheniformis ◽  
High Efficiency ◽  
High Yield ◽  
Chromosomal Integration ◽  
Bacillus Sp ◽  
Strain Development ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
New Strategy ◽  
Amplification Strategy

Abstract Highly efficient preparation of industrially important enzymes depends on development of the genetically stable and high-yield microbial cell factories, which is often a challengeable laboratory hard work. In aims to simplify strain development with high efficiency for enzyme overproduction, a new strategy based on chromosomal integration and amplification in Bacillus sp . was developed. A pair of plasmids, an integrated expression plasmid pUB'-Ex1 and a thermosensitive replicable plasmid pUB-MazF, were constructed. pUB'-Ex1 conditionally self-replicated in Bacillus sp . when the RepF in pUB-MazF expressed. pUB-MazF thermosensitively self-replicated in Bacillus sp . , which was easily cured from the host by inducing MazF expression with IPTG. Bacillus licheniformis BL-UBM that integrated with pUB-MazF was then transformed with pUB'-amyS derived from pUB'-Ex1 by in-frame cloning of amyS encoding a thermophilic α-amylase from Geobacillus stearothermophilus ATCC 31195. The transformant of B. licheniformis BL-UBM with pUB'-amyS was cultivated at 42 o C with the existence of 1 mmol/l IPTG and 500 μg/ml kanamycin and the recombinants with high α-amylase activities were selected. All tested recombinants were extremely high genetic stability. One of which, recombinant BLiS-002, carried five copies of amyS and produced the highest yield of α-amylase. It could yield 50,753 U/ml of α-amylase in a 50-l bioreactor. The strategy developed in this study is of application potential for convenient and quick strain development for industrially important enzyme overexpression.

scholarly journals Atomic Engineering Bifunctional Niobium (V)-based Heterostructure Nanosheet for High Efficiency Lean-Electrolyte Lithium-Sulfur Full Batteries

2021 ◽  
Author(s):  
Haodong Shi ◽  
Jieqiong Qin ◽  
Pengfei Lu ◽  
Cong Dong ◽  
Pratteek Das ◽  
...  
Keyword(s):  
High Efficiency ◽  
Electrode Structure ◽  
Practical Applications ◽  
Dual Functional ◽  
Redox Kinetics ◽  
Minimum Capacity ◽  
Li Ion ◽  
Positive Capacity ◽  
Areal Capacity ◽  
Lithium Sulfur

Abstract High-efficiency lithium-sulfur (Li-S) batteries depend on advanced electrode structure that can attain high sulfur utilization at lean-electrolyte and limited lithium. Herein, a twinborn holey Nb4N5-Nb2O5 heterostructure is designed as a dual-functional host for both redox-kinetics-accelerated sulfur cathode and dendrite-inhibited Li anode simultaneously for long-cycling and lean-electrolyte Li-S full batteries. Benefiting from the accelerative polysulfides anchoring-diffusion converting efficiency and electronic-conducting properties of Nb4N5-Nb2O5, polysulfide-shutting is significantly alleviated. Meanwhile, the lithiophilic nature of holey Nb4N5-Nb2O5 is applied as ion-redistributor for homogeneous Li-ion deposition. Taking advantage of these merits, the Li-S full batteries present the excellent electrochemical properties, including a minimum capacity decay of 0.025% per cycle, and a high areal-capacity of 5.0 mAh cm− 2 at sulfur loading of 6.9 mg cm− 2, corresponding to negative to positive capacity ratio (2.4:1) and electrolyte to sulfur ratio (5.1 µl mg− 1). Therefore, this work opens a new avenue for boosting high-performances Li-S batteries towards practical applications.

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