Development of a horseradish peroxidase-Fenton-like system for the degradation of sulfamethazine under weak acid condition

2021 ◽  
Author(s):  
Hong Liu ◽  
Zaihui Huang ◽  
Chunguang Liu
Keyword(s):  
Horseradish Peroxidase ◽  
Weak Acid ◽  
Acid Condition

Preparation of Amido Modified Silicone in Microemulsion

2008 ◽  
Vol 385-387 ◽  
pp. 497-500
Author(s):  
Jie Sheng Liu ◽  
Shi Qiang Huang ◽  
Shao Peng Wu
Keyword(s):  
Coupling Agent ◽  
Weak Acid ◽  
Correlation Spectroscopy ◽  
Acid Condition ◽  
Photon Correlation ◽  
Ph Values ◽  
Transmission Electron ◽  
Structures And Properties ◽  
Effects Of Ph ◽  
Ft Ir

A translucent amido modified microemulsion was synthesized by the adding of amido silane coupling agent. Transmission electron microscope (TEM), Fourier Transform Infrared Spectra (FT-IR), Photon Correlation Spectroscopy (PCS) were used to characterize the structures and properties of amido microemulsion, respectively. IR spectroscopy confirmed that amido group was successfully introduced into the polysiloxane microemulsion. TEM images indicated that the granules agglomerated heavily when the coupling agent was in a larger content. The effects of PH values, amount of coupling agent on the size and the configuration of the amido microemulsion were investigated. The results indicated that the properties of amido microemulsion were stable when the microemulsion was under weak acid condition (pH=3-7). When the amount of coupling agent increased, the particles size of microemulsion increased and the particle size distribution became narrow.

Aging behavior of nano-CaCO3-modified polypropylene nonwoven fabric composites

2020 ◽  
pp. 096739112090354
Author(s):  
Zongqian Wang ◽  
Yu Li ◽  
Yinchun Fang ◽  
Ganyu Feng ◽  
Jingjing Xu
Keyword(s):  
Corrosion Resistance ◽  
Acid Corrosion ◽  
Filter Material ◽  
Strong Acid ◽  
Nonwoven Fabric ◽  
Weak Acid ◽  
Aging Behavior ◽  
Acid Condition ◽  
Uv Aging ◽  
Fabric Composite

Nano-CaCO3-modified polypropylene (PP) nonwoven fabric composite is widely used as the filter material which could overcome the poor lightfastness and low weight of the pure one, while its aging performances would be influenced. In this article, the aging behavior of the nano-CaCO3-modified PP nonwoven fabric composite was investigated. At first, the difference between untreated and CaCO3-modified PP nonwoven fabric was analyzed by scanning electron microscopy, thermogravimetric/differential thermal analysis, and X-ray diffraction. Then the aging behaviors of these two PP nonwoven fabrics were studied in detail. The results showed that the PP nonwoven fabric exhibited good acid corrosion resistance. On the contrary, acid corrosion resistance of the modified PP nonwoven fabric was poor; although its weight and strength didn’t change under weak acid condition, its weight and strength loss both increased obviously under medium and strong acid condition with the prolongation of corrosion time. Due to the ultraviolet (UV) energy dissipation of nano-CaCO3 particles, the UV aging resistance of the modified PP nonwoven fabric was improved, and the compound UV aging under weak acid condition was also superior to that of the untreated PP fabric. This article will provide experimental and theoretical base for the structural regulation of CaCO3-modified PP fibers.

Effects of Vincristine on Endothelial Microtubules in the Spinal Cord

1976 ◽  
Vol 34 ◽  
pp. 58-59
Author(s):  
John L. Beggs ◽  
John D. Waggener ◽  
Wanda Miller
Keyword(s):  
Spinal Cord ◽  
Biological Activity ◽  
Horseradish Peroxidase ◽  
Transport Mechanism ◽  
Vasogenic Edema ◽  
Vesicular Transport ◽  
Close Proximity

Microtubules (MT) are versatile organelles participating in a wide variety of biological activity. MT involvement in the movement and transport of cytoplasmic components has been well documented. In the course of our study on trauma-induced vasogenic edema in the spinal cord we have concluded that endothelial vesicles contribute to the edema process. Using horseradish peroxidase as a vascular tracer, labeled endothelial vesicles were present in all situations expected if a vesicular transport mechanism was in operation. Frequently,labeled vesicles coalesced to form channels that appeared to traverse the endothelium. The presence of MT in close proximity to labeled vesicles sugg ested that MT may play a role in vesicular activity.

Comparative strengths and weaknesses of peroxidase and colloidal gold in pre- and post-embedding immunolabeling techniques

1987 ◽  
Vol 45 ◽  
pp. 1002-1005
Author(s):  
D. R. Abrahamson ◽  
P. L. St.John ◽  
E. W. Perry
Keyword(s):  
Electron Microscopy ◽  
Horseradish Peroxidase ◽  
Light Microscopy ◽  
Colloidal Gold ◽  
Light Microscope ◽  
Ultrastructural Localization ◽  
The Other ◽  
Quantitative Studies ◽  
Dense Suspension ◽  
Antigen Localization

Antibodies coupled to tracers for electron microscopy have been instrumental in the ultrastructural localization of antigens within cells and tissues. Among the most popular tracers are horseradish peroxidase (HRP), an enzyme that yields an osmiophilic reaction product, and colloidal gold, an electron dense suspension of particles. Some advantages of IgG-HRP conjugates are that they are readily synthesized, relatively small, and the immunolabeling obtained in a given experiment can be evaluated in the light microscope. In contrast, colloidal gold conjugates are available in different size ranges and multiple labeling as well as quantitative studies can therefore be undertaken through particle counting. On the other hand, gold conjugates are generally larger than those of HRP but usually can not be visualized with light microscopy. Concern has been raised, however, that HRP reaction product, which is exquisitely sensitive when generated properly, may in some cases distribute to sites distant from the original binding of the conjugate and therefore result in spurious antigen localization.

Unlabeled Antibody Immunocytochemistry Applied to Murine Mammary Tumor Cells

1975 ◽  
Vol 33 ◽  
pp. 390-391
Author(s):  
Wm. J. Arnold ◽  
J. Russo ◽  
H. D. Soule ◽  
M. A. Rich
Keyword(s):  
Horseradish Peroxidase ◽  
Mammary Tumor ◽  
Covalent Binding ◽  
Petri Dish ◽  
Gamma Chain ◽  
Mammary Tumor Virus ◽  
Mammary Tumor Cells ◽  
Murine Mammary Tumor ◽  
Tumor Virus ◽  
Unlabeled Antibody

Our studies of mammary tumor virus have included the application of the unlabeled antibody enzyme method of Sternberger to mammary tumor derived mouse cells in culture and observation with an electron microscope. The method avoids the extravagance of covalent binding of indicator molecules (horseradish peroxidase) with precious antibody locator molecules by relying instead upon specific antibody-antigen linkages. Our reagents included: Primary Antibody, rabbit anti-murine mammary tumor virus (MuMTV) which was antiserum 113 AV-2; Secondary Antibody, goat anti-rabbit IgG gamma chain (Cappel Laboratories); andthe Indicator, rabbit anti-horseradish peroxidase - horseradish peroxidase complex (PAP) (Cappel Labs.). Dilutions and washes were made in 0.05 M Tris 0.15 M saline buffered to pH 7.4. Cell monolayers, after light fixation in glutaraldehyde, were incubated in place by a protocol adapted from Sternberger and Graham and Karnovsky, then embedded by our usual method for monolayers. Reagents were confined to specific areas by neoprene 0-rings (Parker Seal Co.) reducing the amount of reagent needed to 50 microliters, 1/6th of that required to wet a 35 mm petri dish.

Henderson Hasselbalch Relationship and Weak Acid Titration

2019 ◽  
Author(s):  
Marc Blétry
Keyword(s):  
Theoretical Framework ◽  
Weak Acid ◽  
Acid Base ◽  
Sensitive Technique ◽  
Chemistry Students ◽  
Advanced Students ◽  
Acid Titration ◽  
Acid Base Titration ◽  
Analytical Expressions

Henderson-Hasselbalch relation is generally the simplified theoretical framework used to introduce students to acid-base titration. However, it is not always valid and its limitations should be made clear to chemistry students. The appropriate parameter to evaluate its validity is K a /C 0 , in connection with Ostwald dilution law. For more advanced students, it is possible to deduce analytical expressions that always fit accurately acid-base titrations and allow an evaluation of Henderson Hasselbalch relation. Gran plot appears as a particularly sensitive technique to the breakdown of Henderson Hasselbalch relation.

Use of Low Pressure Mercury Lamps for the Photodecomposition of Iron Cyanide in Gold Mill Effluents

1985 ◽  
Vol 20 (2) ◽  
pp. 111-119 ◽  
Author(s):  
J.H. Carey ◽  
S.A. Zaidi
Keyword(s):  
Ultraviolet Light ◽  
High Intensity ◽  
Experimental Evaluation ◽  
Low Pressure ◽  
Pilot Scale ◽  
Weak Acid ◽  
Potassium Ferricyanide ◽  
Technical Feasibility ◽  
Iron Cyanide ◽  
Scale Test

Abstract The use of ultraviolet light (UV) from low pressure mercury lamps for destroying iron cyanide in synthetic and actual gold mill effluents was evaluated in this study. For the light intensities used in this study, UV irradiation was not able to efficiently destroy cyanide. However, it converted iron cyanide to a weak acid dissociable form which was destroyed by chlorine. Data from several bench-scale tests and one pilot scale test were used to estimate quantum efficiencies (moles iron cyanide destroyed/einstein). These efficiencies ranged from 0.2% to 1%; approximately 30% to 90% lower than those reported in the literature for potassium ferricyanide. The data collected during the study demonstrated the technical feasibility of using UV in conjunction with chlorination for destroying iron cyanide in gold mill effluents. However, low pressure mercury lamps do not appear to be a practical UV source for this purpose. Irradiation with high intensity lamps may be more practical and is recommended for experimental evaluation.

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