Quantitative analysis of acylated oleanane-type triterpene saponins, chakasaponins I–III and floratheasaponins A–F, in the flower buds of Camellia sinensis from different regional origins

Using the recently developed two analytical protocols, distributions were analyzed of five catechins (1–5), ten flavonoids (6—15), caffeine (16), and nine saponins (17—25) in 12 samples of flower buds of Camellia sinensis (L.) O. Kuntze, collected at different points in Taiwan. Characteristic tendencies with respect to the distribution of these constituents were observed according to the region of collection. Among the catechins, (–)-epigallocatechin 3- O-gallate (5) was the major constituent in all the samples. Notably, the content of 5 was higher in samples from the mountain regions in the middle and northern Taiwan than in samples from other regions. As for the principal flavonoids, the content of 10 was higher than that of 11 in most of the samples except those of Sijichun tea. For the saponin contents, the following trends were observed: (1) contents of chakasaponins I—III (17—19) were higher in samples from the mountain region in the middle and northern areas; and (2) contents of floratheasaponins A—F (20—25) were higher in the samples from central and southern areas.

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Flavonol glycosides with lipid accumulation inhibitory activity and simultaneous quantitative analysis of 15 polyphenols and caffeine in the flower buds of Camellia sinensis from different regions by LCMS

Food Chemistry ◽

10.1016/j.foodchem.2013.02.079 ◽

2013 ◽

Vol 140 (1-2) ◽

pp. 353-360 ◽

Cited By ~ 20

Author(s):

Toshio Morikawa ◽

Kiyofumi Ninomiya ◽

Sohachiro Miyake ◽

Yoshinobu Miki ◽

Masaki Okamoto ◽

...

Keyword(s):

Quantitative Analysis ◽

Camellia Sinensis ◽

Inhibitory Activity ◽

Lipid Accumulation ◽

Flavonol Glycosides ◽

Flower Buds

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Acylated Oleanane-Type Triterpene Saponins with Acceleration of Gastrointestinal Transit and Inhibitory Effect on Pancreatic Lipase from Flower Buds of Chinese Tea Plant (Camellia sinensis)

Chemistry & Biodiversity ◽

10.1002/cbdv.200800153 ◽

2009 ◽

Vol 6 (6) ◽

pp. 903-915 ◽

Cited By ~ 38

Author(s):

Masayuki Yoshikawa ◽

Sachiko Sugimoto ◽

Yasuyo Kato ◽

Seikou Nakamura ◽

Tao Wang ◽

...

Keyword(s):

Camellia Sinensis ◽

Pancreatic Lipase ◽

Gastrointestinal Transit ◽

Inhibitory Effect ◽

Tea Plant ◽

Triterpene Saponins ◽

Flower Buds

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Bioactive Saponins and Glycosides. XXV. Acylated Oleanane-Type Triterpene Saponins from the Seeds of Tea Plant (Camellia sinensis)

Chemical and Pharmaceutical Bulletin ◽

10.1248/cpb.55.57 ◽

2007 ◽

Vol 55 (1) ◽

pp. 57-63 ◽

Cited By ~ 41

Author(s):

Masayuki Yoshikawa ◽

Toshio Morikawa ◽

Seikou Nakamura ◽

Ning Li ◽

Xian Li ◽

...

Keyword(s):

Camellia Sinensis ◽

Tea Plant ◽

Triterpene Saponins

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Simultaneous Determination and Quantification of Triterpene Saponins from Camellia sinensis Seeds Using UPLC-PDA-QTOF-MS/MS

Molecules ◽

10.3390/molecules24203794 ◽

2019 ◽

Vol 24 (20) ◽

pp. 3794 ◽

Cited By ~ 6

Author(s):

Xuejin Wu ◽

Lingyan Jia ◽

Jiafan Wu ◽

Yawen Liu ◽

Hyunuk Kang ◽

...

Keyword(s):

Sulfuric Acid ◽

Camellia Sinensis ◽

High Performance ◽

Biological Properties ◽

Array Detector ◽

Fragmentation Pattern ◽

Triterpene Saponins ◽

Chemical Structures ◽

Flight Mass Spectrometry ◽

Saponin Content

Saponins in the Camellia sinensis seeds have a broad spectrum of biological properties and application potentials. However, up to now, no chromatographic methods have been developed to provide full fingerprinting and quality assurance for these saponins. This research aimed to develop a novel method to tentatively identify and quantify saponins in C. sinensis seeds by ultra-high-performance liquid chromatography coupled with photo-diode array detector and quadrupole time-of-flight mass spectrometry (UPLC-PDA-QTOF-MS/MS), and compare it with the classic vanillin-sulfuric acid assay. Fifty-one triterpene saponins, including six potentially new compounds, were simultaneously detected by UPLC-PDA-MS/MS, and their chemical structures were speculated according to the retention behavior and fragmentation pattern. The total saponin content in the crude extract and the purified saponin fraction of C. sinensis seeds were quantified to be 19.57 ± 0.05% (wt %) and 41.68 ± 0.09% (wt %) respectively by UPLC-PDA at 210 nm, while the corresponding values were determined to be 43.11 ± 3.17% (wt %) and 56.60 ± 5.79% (wt %) respectively by the vanillin-sulfuric acid assay. The developed UPLC-PDA -MS/MS method could determine specified saponins, and is more reliable for quantifying the C. sinensis seed saponins than the classic spectrophotometric method. It is of great significance for the future investigations and applications of these saponins.

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