A GATA-type transcription factor AcAREB for nitrogen metabolism is involved in regulation of cephalosporin biosynthesis in Acremonium chrysogenum

Autotoxicity of root exudates is one of the main reasons for consecutive monoculture problem (CMP) in cucumber under greenhouse cultivation. Rootstock grafting may improve the tolerance of cucumber plants to autotoxic stress. To verify the enhanced tolerance to autotoxic stress and illuminate relevant molecular mechanism, a transcriptomic comparative analysis was performed between rootstock grafted (RG) and non-grafted (NG) cucumber plants by a simulation of exogenous cinnamic acid (CA). The present study confirmed that relatively stable plant growth, biomass accumulation, chlorophyll content, and photosynthesis was observed in RG than NG under CA stress. We identified 3647 and 2691 differentially expressed genes (DEGs) in NG and RG cucumber plants when compared to respective control, and gene expression patterns of RNA-seq was confirmed by qRT-PCR. Functional annotations revealed that DEGs response to CA stress were enriched in pathways of plant hormone signal transduction, MAPK signaling pathway, phenylalanine metabolism, and plant-pathogen interaction. Interestingly, the significantly enriched pathway of photosynthesis-related, carbon and nitrogen metabolism only identified in NG, and most of DEGs were down-regulated. However, most of photosynthesis, Calvin cycle, glycolysis, TCA cycle, and nitrogen metabolism-related DEGs exhibited not or slightly down-regulated in RG. In addition, several stress-related transcription factor families of AP2/ERF, bHLH, bZIP, MYB. and NAC were uniquely triggered in the grafted cucumbers. Overall, the results of this study suggest that rootstock grafting improve the tolerance of cucumber plants to autotoxic stress by mediating down-regulation of photosynthesis, carbon, and nitrogen metabolism-related DEGs and activating the function of stress-related transcription factor. The transcriptome dataset provides an extensive sequence resource for further studies of autotoxic mechanism at molecular level.

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Use of bimolecular fluorescence complementation to demonstrate transcription factor interaction in nuclei of living cells from the filamentous fungus Acremonium chrysogenum

Current Genetics ◽

10.1007/s00294-004-0546-0 ◽

2004 ◽

Vol 47 (2) ◽

pp. 132-138 ◽

Cited By ~ 51

Author(s):

Birgit Hoff ◽

Ulrich K�ck

Keyword(s):

Transcription Factor ◽

Filamentous Fungus ◽

Living Cells ◽

Bimolecular Fluorescence Complementation ◽

Acremonium Chrysogenum ◽

Transcription Factor Interaction ◽

Factor Interaction ◽

Fluorescence Complementation ◽

Bimolecular Fluorescence

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Identification of the Main Regulator Responsible for Synthesis of the Typical Yellow Pigment Produced by Trichoderma reesei

Applied and Environmental Microbiology ◽

10.1128/aem.01408-16 ◽

2016 ◽

Vol 82 (20) ◽

pp. 6247-6257 ◽

Cited By ~ 29

Author(s):

Christian Derntl ◽

Alice Rassinger ◽

Ewald Srebotnik ◽

Robert L. Mach ◽

Astrid R. Mach-Aigner

Keyword(s):

Transcription Factor ◽

Secondary Metabolism ◽

Trichoderma Reesei ◽

Penicillium Chrysogenum ◽

Yellow Pigment ◽

Mass Spectrometry Analysis ◽

Acremonium Chrysogenum ◽

Pigment Formation ◽

Content Type ◽

Cluster A

ABSTRACTThe industrially used ascomyceteTrichoderma reeseisecretes a typical yellow pigment during cultivation, while otherTrichodermaspecies do not. A comparative genomic analysis suggested that a putative secondary metabolism cluster, containing two polyketide-synthase encoding genes, is responsible for the yellow pigment synthesis. This cluster is conserved in a set of rather distantly related fungi, includingAcremonium chrysogenumandPenicillium chrysogenum. In an attempt to silence the cluster inT. reesei, two genes of the cluster encoding transcription factors were individually deleted. For a complete genetic proof-of-function, the genes were reinserted into the genomes of the respective deletion strains. The deletion of the first transcription factor (termed yellow pigment regulator 1 [Ypr1]) resulted in the full abolishment of the yellow pigment formation and the expression of most genes of this cluster. A comparative high-pressure liquid chromatography (HPLC) analysis of supernatants of theypr1deletion and its parent strain suggested the presence of several yellow compounds inT. reeseithat are all derived from the same cluster. A subsequent gas chromatography/mass spectrometry analysis strongly indicated the presence of sorbicillin in the major HPLC peak. The presence of the second transcription factor, termed yellow pigment regulator 2 (Ypr2), reduces the yellow pigment formation and the expression of most cluster genes, including the gene encoding the activator Ypr1.IMPORTANCETrichoderma reeseiis used for industry-scale production of carbohydrate-active enzymes. During growth, it secretes a typical yellow pigment. This is not favorable for industrial enzyme production because it makes the downstream process more complicated and thus increases operating costs. In this study, we demonstrate which regulators influence the synthesis of the yellow pigment. Based on these data, we also provide indication as to which genes are under the control of these regulators and are finally responsible for the biosynthesis of the yellow pigment. These genes are organized in a cluster that is also found in other industrially relevant fungi, such as the two antibiotic producersPenicillium chrysogenumandAcremonium chrysogenum. The targeted manipulation of a secondary metabolism cluster is an important option for any biotechnologically applied microorganism.

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Disruption of the nitrogen regulatory gene AcareA in Acremonium chrysogenum leads to reduction of cephalosporin production and repression of nitrogen metabolism

Fungal Genetics and Biology ◽

10.1016/j.fgb.2013.10.006 ◽

2013 ◽

Vol 61 ◽

pp. 69-79 ◽

Cited By ~ 22

Author(s):

Jinyang Li ◽

Yuanyuan Pan ◽

Gang Liu

Keyword(s):

Nitrogen Metabolism ◽

Regulatory Gene ◽

Acremonium Chrysogenum

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CPCR1, but not its interacting transcription factor AcFKH1, controls fungal arthrospore formation in Acremonium chrysogenum

Molecular Microbiology ◽

10.1111/j.1365-2958.2005.04626.x ◽

2005 ◽

Vol 56 (5) ◽

pp. 1220-1233 ◽

Cited By ~ 32

Author(s):

Birgit Hoff ◽

Esther K. Schmitt ◽

Ulrich Kück

Keyword(s):

Transcription Factor ◽

Acremonium Chrysogenum

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The extended regulon of the Escherichia coli LgoR transcription factor includes genes for sugar acid and nitrogen metabolism

New Biotechnology ◽

10.1016/j.nbt.2016.06.1334 ◽

2016 ◽

Vol 33 ◽

pp. S177

Author(s):

Maria Tutukina ◽

Uliana Shvyreva ◽

Inna Suvorova ◽

Diana Gagarinskaya ◽

Olga Ozoline ◽

...

Keyword(s):

Escherichia Coli ◽

Transcription Factor ◽

Nitrogen Metabolism

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Ipa1 Improves Rice Drought Tolerance at Seedling Stage Mainly Through Activating Abscisic Acid Pathway

10.21203/rs.3.rs-733123/v1 ◽

2021 ◽

Author(s):

Menghao Zhu ◽

Yonggang He ◽

Mingqiang Zhu ◽

Ayaz Ahmad ◽

Shuang Xu ◽

...

Keyword(s):

Transcription Factor ◽

Drought Tolerance ◽

Nitrogen Metabolism ◽

Crop Production ◽

Plant Architecture ◽

Biotic Resistance ◽

Rice Seedling ◽

Seedling Stage ◽

Luciferase Assay ◽

Aba Biosynthesis

Abstract Drought is a major abiotic stress to crop production. IPA1 (IDEAL PLANT ARCHITECTURE 1)/OsSPL14 encodes a transcription factor and has been reported to function in both rice ideal plant architecture and biotic resistance. Here, with a pair of IPA1/ipa1-NILs (Near Iso-genic Lines), we found that ipa1 could significantly improve rice drought tolerance at seedling stage. The ipa1 plants had a better-developed root system and smaller leaf stomatal aperture. Analysis of carbon-nitrogen metabolism-associated enzyme activity, gene expression, and metabolic profile indicated that ipa1 could tip the carbon-nitrogen metabolism balance towards an increased carbon metabolism pattern. In both the control and PEG-treated conditions, ABA content in the ipa1 seedlings was significantly higher than that in the IPA1 seedlings. Expression of the ABA biosynthesis genes was detected to be up-regulated, whereas the expression of ABA catabolism genes was down-regulated in the ipa1 seedlings. In addition, based on yeast one-hybrid assay and dual-luciferase assay, IPA1 was found to directly activate the promoter activity of OsHOX12, a transcription factor promoting ABA biosynthesis, and OsNAC52, a positive regulator of the ABA pathway. The expression of OsHOX12 and OsNAC52 was significantly up-regulated in the ipa1 plants. Combined with the previous studies, our results suggested that ipa1 could improve rice seedling drought tolerance mainly through activating the ABA pathway and that regulation of the ipa1-mediated ABA pawthway will be an important strategy for improving drought resistance of rice.

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