Preparation and Characterization of Poly Lactic Acid/Graphene Oxide/Nerve Growth Factor Scaffold with Electrical Stimulation for Peripheral Nerve Regeneration in vitro

The usage of hollow nerve conduits shows inferior recovery effect on the repair of peripheral nerve defects. In this study, a biocompatible and biodegradable pH-induced injectable chitosan–hyaluronic acid hydrogel for nerve growth factor encapsulation and sustained release was developed as the fillers in the lumen of hollow nerve conduit to reform its microenvironment for peripheral nerve regeneration. The physicochemical properties of hydrogel were characterized by gelation time, Fourier transform infrared spectroscopy, scanning electron microscopy, compressive modulus, porosity, swelling ratio, and in vitro degradation. The in vitro nerve growth factor release profiles and cell evaluation were also investigated. The results show that the structure of chitosan–hyaluronic acid hydrogel is composed of interconnected channels with a controllable pore diameter ranging from 20 to 100 µm. The hydrogel can be degraded more than 70% within 8 weeks in vitro and is available for nerve growth factor sustained release. The chitosan–hyaluronic acid/nerve growth factor hydrogel is non-toxic and suitable for adhesion and proliferation of nerve cells and capable of maintaining nerve growth factor activity. Therefore, it could be a promising intraluminal filler of nerve conduits for peripheral nerve regeneration in neural tissue engineering.

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Preparation and characterization of phosphorylated graphene oxide grafted with poly(L‐lactide) and its effect on the crystallization, rheological behavior, and performance of poly (lactic acid)

Polymers for Advanced Technologies ◽

10.1002/pat.4717 ◽

2019 ◽

Vol 30 (11) ◽

pp. 2846-2859 ◽

Cited By ~ 1

Author(s):

Li Yang ◽

Weijun Zhen

Keyword(s):

Graphene Oxide ◽

Lactic Acid ◽

Rheological Behavior ◽

Poly Lactic Acid ◽

And Performance

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Preparation and in vitro characterization of paclitaxel containing poly(lactic acid co-castor oil)-based nanodispersions

Journal of Drug Delivery Science and Technology ◽

10.1016/s1773-2247(13)50063-3 ◽

2013 ◽

Vol 23 (5) ◽

pp. 439-444 ◽

Cited By ~ 3

Author(s):

J.G. Hiremath ◽

A. Rajeshkumar ◽

D. Ickowicz ◽

A.J. Domb

Keyword(s):

Lactic Acid ◽

Castor Oil ◽

Poly Lactic Acid ◽

In Vitro Characterization

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In Vitro Characterization of Poly(Lactic Acid)/ Poly(Hydroxybutyrate)/ Thermoplastic Starch Blends for Tissue Engineering Application

Cell Transplantation ◽

10.1177/09636897211021003 ◽

2021 ◽

Vol 30 ◽

pp. 096368972110210

Author(s):

Martina Culenova ◽

Ivana Birova ◽

Pavol Alexy ◽

Paulina Galfyova ◽

Andreas Nicodemou ◽

...

Keyword(s):

Tissue Engineering ◽

Lactic Acid ◽

Thermoplastic Starch ◽

Biological Behavior ◽

Poly Lactic Acid ◽

Cytotoxicity Test ◽

In Vitro Characterization ◽

Custom Made

Complex in vitro characterization of a blended material based on Poly(Lactic Acid), Poly(Hydroxybutyrate), and Thermoplastic Starch (PLA/PHB/TPS) was performed in order to evaluate its potential for application in the field of tissue engineering. We focused on the biological behavior of the material as well as its mechanical and morphological properties. We also focused on the potential of the blend to be processed by the 3D printer which would allow the fabrication of the custom-made scaffold. Several blends recipes were prepared and characterized. This material was then studied in the context of scaffold fabrication. Scaffold porosity, wettability, and cell-scaffold interaction were evaluated as well. MTT test and the direct contact cytotoxicity test were applied in order to evaluate the toxic potential of the blended material. Biocompatibility studies were performed on the human chondrocytes. According to our results, we assume that material had no toxic effect on the cell culture and therefore could be considered as biocompatible. Moreover, PLA/PHB/TPS blend is applicable for 3D printing. Printed scaffolds had highly porous morphology and were able to absorb water as well. In addition, cells could adhere and proliferate on the scaffold surface. We conclude that this blend has potential for scaffold engineering.

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