Primary fibroblast cultures and karyotype analysis for the olive ridley sea turtle (Lepidochelys olivacea)

2013 ◽  
Vol 50 (5) ◽  
pp. 381-383 ◽  
Author(s):  
Tomokazu Fukuda ◽  
Masafumi Katayama ◽  
Kodzue Kinoshita ◽  
Takashi Kasugai ◽  
Hitoshi Okamoto ◽  
...  
Keyword(s):  
Karyotype Analysis ◽  
Sea Turtle ◽  
Olive Ridley ◽  
Primary Fibroblast ◽  
Lepidochelys Olivacea ◽  
Fibroblast Cultures

scholarly journals Chelonicola and Poulinea, two new gomphonemoid diatom genera (Bacillariophyta) living on marine turtles from Costa Rica

Phytotaxa ◽  
2015 ◽  
Vol 233 (3) ◽  
pp. 236 ◽  
Author(s):  
Roksana Majewska ◽  
J. P. Kociolek ◽  
Evan W. Thomas ◽  
Mario De Stefano ◽  
Mario Santoro ◽  
...  
Keyword(s):  
Costa Rica ◽  
Marine Mammals ◽  
Sea Turtles ◽  
Sea Turtle ◽  
New Genera ◽  
Olive Ridley ◽  
Lepidochelys Olivacea ◽  
Marine Turtles ◽  
The Family ◽  
Long Time

Marine mammals such as whales and dolphins have been known for a long time to host a very specific epizoic community on their skin. Less known however is the presence of a similar community on the carapaces of sea turtles. The present study is the first describing new taxa inhabiting sea turtle carapaces. Samples, collected from nesting olive ridley sea turtles (Lepidochelys olivacea) on Ostional Beach (Costa Rica), were studied using light and scanning electron microscopy. Two unknown small-celled gomphonemoid taxa were analysed in more detail and are described as two new genera, closely related to other gomphonemoid genera with septate girdle bands, such as Tripterion, Cuneolus and Gomphoseptatum. Chelonicola Majewska, De Stefano & Van de Vijver gen. nov. has a flat valve face, uniseriate striae composed of more than three areolae, simple external raphe endings, internally a siliceous flap over the proximal raphe endings and lives on mucilaginous stalks. Poulinea Majewska, De Stefano & Van de Vijver gen. nov. has at least one concave valve, uniseriate striae composed of only two elongated areolae, external distal raphe endings covered by thickened siliceous flaps and lives attached to the substrate by a mucilaginous pad. Chelonicola costaricensis Majewska, De Stefano & Van de Vijver sp. nov. and Poulinea lepidochelicola Majewska, De Stefano & Van de Vijver sp. nov. can be separated based on stria structure, girdle structure composed of more than 10 copulae, raphe structure and general valve outline. A cladistics analysis of putative members of the Rhoicospheniaceae indicates that the family is polyphyletic. Chelonicola and Poulinea are sister taxa, and form a monophyletic group with Cuneolus and Tripterion, but are not closely related to Rhoicosphenia, or other genera previously assigned to this family. Features used to help diagnose the family such as symmetry and presence of septa and pseudosepta are homoplastic across the raphid diatom tree of life.

Successful rehabilitation of an oiled sea turtle (Lepidochelys olivacea) affected by the biggest oil spill disaster in Brazil

2021 ◽  
Author(s):  
REM Oliveira ◽  
FLN Attademo ◽  
JS Galvincio ◽  
ACB Freire ◽  
AS Silva ◽  
...  
Keyword(s):  
Oil Spills ◽  
Sea Turtle ◽  
Entire Body ◽  
Normal Range ◽  
Olive Ridley ◽  
Lepidochelys Olivacea ◽  
Second Stage ◽  
Therapeutic Protocol ◽  
Mucous Membranes ◽  
Therapeutic Procedures

The standardisation of protocols and discussion of therapeutic procedures in the rehabilitation of turtles affected by oil spills are necessary to optimise the recovery time and increase the chances of survival of these animals. This study aimed at reporting the processes adopted for the stabilisation, decontamination, rehabilitation and release of an oiled olive ridley sea turtle (Lepidochelys olivacea), rescued alive on September 23, 2019, at Santa Rita Beach, Extremoz municipality, Rio Grande do Norte, Brazil. Its entire body was covered by oil. At first, the animal was mechanically dry cleaned using a gauze soaked in mineral oil in the keratinised regions (carapace and plastron) and a gauze soaked in vegetable oil was used on the oral, nasal, ocular, and cloacal mucous membranes. The second stage of the oil removal consisted of washing the animal with heated pressurised water (39 °C) and a neutral detergent using a soft foam sponge. The animal received treatment with antitoxins, antibiotics, analgesics, gastrointestinal protectors, and fluid therapy. After 7 days of treatment, the blood count showed that all the parameters were within the normal range. The oil cleaning process and the therapeutic protocol used in the rehabilitation of the olive ridley sea turtle were efficient.

scholarly journals Expression of aromatase in the embryonic brain of the olive ridley sea turtle (Lepidochelys olivacea), and the effect of bisphenol-A in sexually differentiated embryos

2014 ◽  
Vol 58 (10-11-12) ◽  
pp. 733-741 ◽  
Author(s):  
Patsy Gómez-Picos ◽  
Itzel Sifuentes-Romero ◽  
Horacio Merchant-Larios ◽  
Rubí Hernández-Cornejo ◽  
Verónica Díaz-Hernández ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Sea Turtle ◽  
Embryonic Brain ◽  
Olive Ridley ◽  
Lepidochelys Olivacea

scholarly journals Study on the etiology of fibropapillomatosis of olive ridley sea turtles (Lepidochelys olivacea) nesting in the National Wildlife Refuge at Ostional, Guanacaste, Costa Rica

2013 ◽  
Vol 5 ◽  
pp. 119 ◽  
Author(s):  
Laura Brenes Chaves ◽  
Alexis Berrocal ◽  
Ana I. Meneses ◽  
Carlos Jiménez Sánchez ◽  
Carlos M. Orrego Vásquez
Keyword(s):  
Sea Turtles ◽  
Cytoplasmic Inclusion ◽  
Blood Chemistry ◽  
Sea Turtle ◽  
Olive Ridley ◽  
Blood Samples ◽  
Lepidochelys Olivacea ◽  
Wildlife Refuge ◽  
Para Determinar ◽  
Olive Ridley Sea Turtles

Sea turtle fibropapillomatosis is an emerging disease characterized by a proliferation of cutaneous papillomas, fibromas, and fibropapillomas and occasional visceral fibromas. This paper aims to contribute tothe etiology of fibropapillomatosis in olive ridley sea turtles (Lepidochelys olivacea) nesting in OstionalNational Wildlife Refuge. Twenty six olive ridley turtles with cutaneous fibropapilloma were sampled and24 healthy olive ridley turtles served as controls. Biopsies were taken of the cutaneous tumors in sick seaturtles, as well as skin biopsies from control subjects, and blood samples were collected from all turtles.Tumorous samples and skin samples were microscopically analyzed in order to differentiate the histologicalfactors resulting from the disease pathogenesis, where the main histological findings were papillaryepidermal hyperplasia, orthokeratotic hyperkeratosis, spirorchid-like eggs in the dermis, and eosinophilic cytoplasmic inclusion. Hematology and blood chemistry studies were conducted on blood samples, andMCHC, heterophils, lymphocytes, monocytes, AST, total protein, albumin and globulin values were significantlydifferent between healthy turtles and turtles with tumors. A PCR test was also conducted in thesamples to determine the presence of herpesvirus and papillomavirus as possible etiologic agents, wherethe papilomavirus was absent in all the samples, while the herpesvirus was present in 69.23% of the tumors,this being the most probable etiological agent of fibropapillomatosis.La fibropapilomatosis de la tortuga marina es una enfermedad emergente caracterizada por múltiples papilomas, fibromas y fibropapilomas cutáneos, así como ocasionales fibromas viscerales. El presente trabajo tiene como objetivo contribuir a la etiología de la fibropapilomatosis en la tortuga lora (Lepidochelys olivacea) que anida en el Refugio Nacional de Vida Silvestre Ostional. Se muestrearon 26 tortugas lora con fibropapilomas cutáneos y 24 tortugas lora sanas que sirvieron de control. Se tomaron biopsias excisionales de los tumores cutáneos de las tortugas enfermas y biopsias de piel de las tortugas control, además se recolectaron muestras de sangre de todas las tortugas. Las muestras tumorales y de piel se analizaron microscópicamente para diferenciar los factores histológicos que resultan de la patogénesis de la enfermedad, donde los hallazgos histopatológicos principales en los fibropapilomas fueron: crecimiento papiliforme, hiperqueratosis ortoqueratótica, huevos de parásitos similares a espiróquidos en la capa dérmica e inclusión eosinofílica citoplasmática. A las muestras de sangre se les realizó análisis hematológico y de química sanguínea, donde los valores de CHCM, heterófilos, linfocitos, monocitos, AST, proteínas totales, albúmina y globulinas resultaron significativamente diferentes entre las tortugas sanas y las tortugas con tumores. Se realizó PCR a las muestras para determinar la presencia de genoma de herpesvirus y papilomavirus como posibles agentes etiológicos, donde el papilomavirus estuvo ausente en la totalidad de las muestras, mientras el virus Herpes se presentó en el 69.23% de los tumores posicionándose como el posible agente etiológico de la enfermedad.

First Record of the Olive Ridley Sea Turtle Lepidochelys olivacea (Reptilia: Testudines: Cheloniidae) from South Korea

2019 ◽  
Vol 38 (2) ◽  
pp. 153
Author(s):  
Il-Hun Kim ◽  
Chang-Ho Yi ◽  
Jeong-Hyun Lee ◽  
Daesik Park ◽  
In-Young Cho ◽  
...  
Keyword(s):  
South Korea ◽  
First Record ◽  
Sea Turtle ◽  
Olive Ridley ◽  
Lepidochelys Olivacea

scholarly journals Multiple paternity and female-biased mutation at a microsatellite locus in the olive ridley sea turtle (Lepidochelys olivacea)

Heredity ◽  
2002 ◽  
Vol 89 (2) ◽  
pp. 107-113 ◽  
Author(s):  
W E J Hoekert ◽  
H Neuféglise ◽  
A D Schouten ◽  
S B J Menken
Keyword(s):  
Microsatellite Locus ◽  
Multiple Paternity ◽  
Sea Turtle ◽  
Olive Ridley ◽  
Lepidochelys Olivacea

Multiple-antibiotic resistance of Enterococcus faecalis in an endangered olive ridley sea turtle (Lepidochelys olivacea): A case report

2019 ◽  
Author(s):  
Ming-An Tsai ◽  
Chao-Chin Chang ◽  
Tsung-Hsien Li
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Enterococcus Faecalis ◽  
System Analysis ◽  
Sea Turtle ◽  
Resistant Bacteria ◽  
Multiple Antibiotic Resistance ◽  
Olive Ridley ◽  
Lepidochelys Olivacea ◽  
Resistance Patterns

There has recently been an alarming increase in the prevalence of antibiotic resistant bacteria in marine environments. In this study, an Enterococcus sp. was isolated from bacteriologic cultures of the elbow joint of an olive ridley turtle (Lepidochelys olivacea ). The enterococci isolate was identified at the species level as Enterococcus faecalis using PCR and the RAPID ID 32 STREP system. Analysis of the antimicrobial resistance patterns and the antibiotic-resistance genes of the E. faecalis isolated in this study revealed multiple-antibiotic-resistance (MAR). Specifically, the isolate presented resistance to doxycycline, enrofloxacin, erythromycin, neomycin, oxytetracycline, gentamicin, amikacin, ciprofloxacin, spiramycin, chloramphenicol, ceftiofur, and azithromycin. We also identified the tetracycline resistance gene tet(M) in the E. faecalis isolate. Further research into the antimicrobial resistance patterns of Enterococcus spp. should be conducted to promote the conservation of sea turtles.

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