Brain lipid binding protein mediates the proliferation of human glioblastoma cells by regulating ERK1/2 signaling pathway in vitro

2017 ◽  
Vol 54 (2) ◽  
pp. 156-162 ◽  
Author(s):  
Wei Tian ◽  
Jinhong Shi ◽  
Jianbing Qin ◽  
Guohua Jin ◽  
Xiao Han ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Binding Protein ◽  
Lipid Binding ◽  
Brain Lipid ◽  
Glioblastoma Cells ◽  
Human Glioblastoma ◽  
Lipid Binding Protein ◽  
Human Glioblastoma Cells

scholarly journals A New Pathway Promotes Adaptation of Human Glioblastoma Cells to Glucose Starvation

Cells ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 1249
Author(s):  
Alberto Azzalin ◽  
Francesca Brambilla ◽  
Eloisa Arbustini ◽  
Katia Basello ◽  
Attilio Speciani ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Membrane Trafficking ◽  
Adaptor Protein ◽  
Glucose Starvation ◽  
Glioblastoma Cells ◽  
Glucose Addition ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cells ◽  
Inducible Protein

Adaptation of glioblastoma to caloric restriction induces compensatory changes in tumor metabolism that are incompletely known. Here we show that in human glioblastoma cells maintained in exhausted medium, SHC adaptor protein 3 (SHC3) increases due to down-regulation of SHC3 protein degradation. This effect is reversed by glucose addition and is not present in normal astrocytes. Increased SHC3 levels are associated to increased glucose uptake mediated by changes in membrane trafficking of glucose transporters of the solute carrier 2A superfamily (GLUT/SLC2A). We found that the effects on vesicle trafficking are mediated by SHC3 interactions with adaptor protein complex 1 and 2 (AP), BMP-2-inducible protein kinase and a fraction of poly ADP-ribose polymerase 1 (PARP1) associated to vesicles containing GLUT/SLC2As. In glioblastoma cells, PARP1 inhibitor veliparib mimics glucose starvation in enhancing glucose uptake. Furthermore, cytosol extracted from glioblastoma cells inhibits PARP1 enzymatic activity in vitro while immunodepletion of SHC3 from the cytosol significantly relieves this inhibition. The identification of a new pathway controlling glucose uptake in high grade gliomas represents an opportunity for repositioning existing drugs and designing new ones.

An in vitro study on the antitumor effect of sonodynamic therapy using sinoporphyrin sodium on human glioblastoma cells

Ultrasonics ◽  
2021 ◽  
Vol 110 ◽  
pp. 106272
Author(s):  
Yuanyuan Shen ◽  
Yiling Chen ◽  
Yongpeng Huang ◽  
Xiaojun Zeng ◽  
Lanhui Huang ◽  
...  
Keyword(s):  
Antitumor Effect ◽  
In Vitro Study ◽  
Vitro Study ◽  
Glioblastoma Cells ◽  
Sonodynamic Therapy ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cells

Down-regulation of ribosomal protein S15A inhibits proliferation of human glioblastoma cells in vivo and in vitro via AKT pathway

Tumor Biology ◽  
2015 ◽  
Vol 37 (4) ◽  
pp. 4979-4990 ◽  
Author(s):  
Yiqun Yao ◽  
Yongjian Liu ◽  
Xiupeng Lv ◽  
Bin Dong ◽  
Feng Wang ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Akt Pathway ◽  
Glioblastoma Cells ◽  
Down Regulation ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cells
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