In vitro antifungal activity of some traditional Persian medicinal plants on pathogenic fungi

Aims: This study is aimed to evaluate the in vitro antifungal activity effect of the crude aqueous extract (CAE), hydrolate (HY) and essential oil (EO) of Corymbia citriodora, Cymbopogon citratus, Cymbopogon flexuosus and Curcuma longa against the phytopathogenic fungi Alternaria steviae, Botryosphaeria dothidea, Colletotrichum gloeosporioides and Sclerotium rolfsii, and assess, in situ, the effectiveness of CAE of medicinal plants in reducing the severity of the cucumber anthracnose. Methodology: The EOs and HYs were obtained by hydrodistillation. The CAEs were prepared by the turbolysis method. Mycelial growth of the fungi was measured daily, by the diametrically opposite method. In the in vivo test, the CAEs were sprayed on the cotyledon leaves of healthy cucumber plants with three days after were inoculated with C. lagenarium. The severity of assessment of the disease was based on a scale of notes. Results: The medicinal plants studied showed antifungal activity against all or almost all pathogens. In general, treatment with CAE and HY of C. longa revealed the highest inhibition against the fungi tested. With the exception of the EO of C. longa, the other EOs showed total inhibition against all the fungi and in all the concentrations tested. Compared to control, in in vivo assays CAE of C. citratus presents a potential for control of cucumber anthracnose reducing the severity of the disease. Conclusion: The medicinal plants studied produce compounds associated with antimicrobial activity.

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Chemical Composition and Antifungal Activity of the Essential Oil of Cotinus coggygria from Marche Region (Italy)

Natural Product Communications ◽

10.1177/1934578x1801300919 ◽

2018 ◽

Vol 13 (9) ◽

pp. 1934578X1801300 ◽

Cited By ~ 1

Author(s):

Daniele Fraternale ◽

Donata Ricci

Keyword(s):

Antifungal Activity ◽

Essential Oil ◽

Pathogenic Fungi ◽

Gas Chromatography Mass Spectrometry ◽

Dilution Method ◽

Agar Dilution Method ◽

Plant Pathogenic Fungi ◽

Cotinus Coggygria ◽

In Vitro Antifungal Activity

The present study reports the results of gas chromatography-mass spectrometry (GC/MS) analyses of the essential oil from flowering aerial parts of Cotinus coggygria Scoop. (Anacardiaceae), as well as its in vitro antifungal activity against nine plant pathogenic fungi. Moreover, the essential oil was evaluated for its antifungal activity using the agar dilution method, and also MICs (minimum inhibitory concentrations) and MFCs (minimum fungicidal concentrations) were determined. The major compounds identified by GC-MS were limonene (49.2%), (Z)-β-ocimene (13.6%), α-pinene (8.8%) and (E)-β-ocimene (5.9%). The oil showed in vitro antifungal activity against some species of the Fusarium genus, Botrytis cinerea, and Alternaria solani. Our study indicates that the oil of C. coggygria could be used as a control agent for plant pathogenic fungi in natural formulations.

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Synthesis and Bio-Evaluation of Natural Butenolides-Acrylate Conjugates

Molecules ◽

10.3390/molecules24071304 ◽

2019 ◽

Vol 24 (7) ◽

pp. 1304 ◽

Cited By ~ 1

Author(s):

Longzhu Bao ◽

Shuangshuang Wang ◽

Di Song ◽

Jingjing Wang ◽

Xiufang Cao ◽

...

Keyword(s):

Mass Spectrometry ◽

Antifungal Activity ◽

13C Nmr ◽

Pathogenic Fungi ◽

Phytopathogenic Fungi ◽

Gibberella Zeae ◽

Ionization Mass ◽

Activity Screening ◽

In Vitro Antifungal Activity

A series of novel 3-aryl-4-hydroxy-2(5H) furanone-acrylate hybrids were designed and synthesized based on the natural butenolides and acrylates scaffolds. The structures of the prepared compounds were characterized by 1H-NMR, 13C-NMR and electrospray ionization mass spectrometry (ESI-MS), and the bioactivity of the target compounds against twelve phytopathogenic fungi was investigated. The preliminary in vitro antifungal activity screening showed that most of the target compounds had moderate inhibition on various pathogenic fungi at the concentration of 100 mg·L−1, and presented broad-spectrum antifungal activities. Further studies also indicated that compounds 7e and 7k still showed some inhibitory activity against Pestallozzia theae, Sclerotinia sclerotiorum and Gibberella zeae on rape plants at lower concentrations, which could be optimized as a secondary lead for further research.

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