scholarly journals In vitro Antifungal Activity of Plant Extracts, Hydrolates and Essential Oils of Some Medicinal Plants and Control of Cucumber Anthracnose

2019 ◽  
pp. 1-9
Author(s):  
Amanda P. Mattos ◽  
Fabricio P. Povh ◽  
Bruna B. Rissato ◽  
Vítor V. Schwan ◽  
Kátia R. F. Schwan-Estrada
Keyword(s):  
Antifungal Activity ◽  
Medicinal Plants ◽  
Curcuma Longa ◽  
Sclerotium Rolfsii ◽  
Phytopathogenic Fungi ◽  
In Vivo Test ◽  
Activity Effect ◽  
In Vitro Antifungal Activity

Aims: This study is aimed to evaluate the in vitro antifungal activity effect of the crude aqueous extract (CAE), hydrolate (HY) and essential oil (EO) of Corymbia citriodora, Cymbopogon citratus, Cymbopogon flexuosus and Curcuma longa against the phytopathogenic fungi Alternaria steviae, Botryosphaeria dothidea, Colletotrichum gloeosporioides and Sclerotium rolfsii, and assess, in situ, the effectiveness of CAE of medicinal plants in reducing the severity of the cucumber anthracnose. Methodology: The EOs and HYs were obtained by hydrodistillation. The CAEs were prepared by the turbolysis method. Mycelial growth of the fungi was measured daily, by the diametrically opposite method. In the in vivo test, the CAEs were sprayed on the cotyledon leaves of healthy cucumber plants with three days after were inoculated with C. lagenarium. The severity of assessment of the disease was based on a scale of notes. Results: The medicinal plants studied showed antifungal activity against all or almost all pathogens. In general, treatment with CAE and HY of C. longa revealed the highest inhibition against the fungi tested. With the exception of the EO of C. longa, the other EOs showed total inhibition against all the fungi and in all the concentrations tested. Compared to control, in in vivo assays CAE of C. citratus presents a potential for control of cucumber anthracnose reducing the severity of the disease. Conclusion: The medicinal plants studied produce compounds associated with antimicrobial activity.

scholarly journals In vitro antifungal activity of three Saudi plant extracts against some Phytopathogenic fungi

2012 ◽  
Vol 52 (4) ◽  
pp. 458-462 ◽  
Author(s):  
Abdul Aziz A. Al-Askar
Keyword(s):  
Antifungal Activity ◽  
Plant Extracts ◽  
Sclerotium Rolfsii ◽  
Ethanolic Extract ◽  
Punica Granatum ◽  
Phytopathogenic Fungi ◽  
Plant Diseases ◽  
High Concentration ◽  
In Vitro Antifungal Activity

Abstract The antifungal activities of ethanolic extracts of three Saudi plants; camel thorn (Alhagi maurorum Medic.), caper (Capparisspinosa L.), and pomegranate (Punica granatum L.) were investigated in vitro against Alternaria alternata, Fusarium oxysporum, Phomadestructiva, Rhizoctonia solani, and Sclerotium rolfsii at concentrations of 0, 3, 6, and 9% (v/v). All tested plant extracts; seeds, roots, and rinds had different degrees of antifungal activity against the tested fungi. When compared with the control, the highest antifungal activity was recorded for camel thorn seeds extract at a concentration of 9%, while, pomegranate rinds extract at 9% came in second. Camel thorn rinds extract came in last even when used at a high concentration. The ethanolic extract of camel thorn seeds may be recommended as a potent bio-fungicide. Extensive studies should be undertaken for the ethanolic extract of camel thorn seeds as a strong antifungal agent against fungal plant diseases.

scholarly journals In Vitro Antifungal Activity of Sanguinarine and Chelerythrine Derivatives against Phytopathogenic Fungi

Molecules ◽  
2012 ◽  
Vol 17 (11) ◽  
pp. 13026-13035 ◽  
Author(s):  
Xin-Juan Yang ◽  
Fang Miao ◽  
Yao Yao ◽  
Fang-Jun Cao ◽  
Rui Yang ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Phytopathogenic Fungi ◽  
Sanguinarine And Chelerythrine ◽  
In Vitro Antifungal Activity

scholarly journals Synthesis, Antifungal Activity and Structure-Activity Relationships of Vanillin Oxime-N-O-Alkanoates

2012 ◽  
Vol 7 (12) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Vivek Ahluwalia ◽  
Nandini Garg ◽  
Birendra Kumar ◽  
Suresh Walia ◽  
Om P. Sati
Keyword(s):  
Antifungal Activity ◽  
Chain Length ◽  
Acyl Chain ◽  
Sclerotium Rolfsii ◽  
Hydroxylamine Hydrochloride ◽  
Macrophomina Phaseolina ◽  
Phytopathogenic Fungi ◽  
Acyl Chlorides ◽  
Acyl Chain Length

Vanillin oxime- N-O-alkanoates were synthesized following reaction of vanillin with hydroxylamine hydrochloride, followed by reaction of the resultant oxime with acyl chlorides. The structures of the compounds were confirmed by IR, 1H, 13C NMR and mass spectral data. The test compounds were evaluated for their in vitro antifungal activity against three phytopathogenic fungi Macrophomina phaseolina, Rhizoctonia solani and Sclerotium rolfsii by the poisoned food technique. The moderate antifungal activity of vanillin was slightly increased following its conversion to vanillin oxime, but significantly increased after conversion of the oxime to oxime- N-O-alkanoates. While vanillin oxime- N-O-dodecanoate with an EC50 value 73.1 μg/mL was most active against M. phaseolina, vanillin oxime- N-O-nonanoate with EC50 of value 66.7 μg/mL was most active against R. solani. The activity increased with increases in the acyl chain length and was maximal with an acyl chain length of nine carbons.

IN VITRO AND IN VIVO ANTIFUNGAL ACTIVITY OF EIGHT MEDICINAL PLANTS AGAINST CITRUS SOUR ROT AGENT GEOTRICHUM CANDIDUM

2015 ◽  
pp. 1563-1570 ◽  
Author(s):  
Idriss Talibi ◽  
Latifa Askarne ◽  
Hassan Boubaker ◽  
El Hassane Boudyach ◽  
Abdellah Ait Ben Oumar
Keyword(s):  
Antifungal Activity ◽  
Medicinal Plants ◽  
Geotrichum Candidum ◽  
Sour Rot ◽  
Citrus Sour Rot ◽  
In Vivo Antifungal Activity

scholarly journals Antifungal Activity In Vitro and In Vivo of Mesquite Extract (Prosopis glandulosa) Against Phytopathogenic Fungi

2020 ◽  
Author(s):  
Arely Nohemí López-Anchondo ◽  
Daniel López-de la Cruz ◽  
Edgar Gutiérrez-Reyes ◽  
José Cristobal Castañeda-Ramírez ◽  
Norma Margarita De la Fuente-Salcido
Keyword(s):  
Antifungal Activity ◽  
Phytopathogenic Fungi ◽  
Prosopis Glandulosa

scholarly journals Synthesis and Bio-Evaluation of Natural Butenolides-Acrylate Conjugates

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1304 ◽  
Author(s):  
Longzhu Bao ◽  
Shuangshuang Wang ◽  
Di Song ◽  
Jingjing Wang ◽  
Xiufang Cao ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Antifungal Activity ◽  
13C Nmr ◽  
Pathogenic Fungi ◽  
Phytopathogenic Fungi ◽  
Gibberella Zeae ◽  
Ionization Mass ◽  
Activity Screening ◽  
In Vitro Antifungal Activity

A series of novel 3-aryl-4-hydroxy-2(5H) furanone-acrylate hybrids were designed and synthesized based on the natural butenolides and acrylates scaffolds. The structures of the prepared compounds were characterized by 1H-NMR, 13C-NMR and electrospray ionization mass spectrometry (ESI-MS), and the bioactivity of the target compounds against twelve phytopathogenic fungi was investigated. The preliminary in vitro antifungal activity screening showed that most of the target compounds had moderate inhibition on various pathogenic fungi at the concentration of 100 mg·L−1, and presented broad-spectrum antifungal activities. Further studies also indicated that compounds 7e and 7k still showed some inhibitory activity against Pestallozzia theae, Sclerotinia sclerotiorum and Gibberella zeae on rape plants at lower concentrations, which could be optimized as a secondary lead for further research.

scholarly journals Strong Antifungal Activity of SS750, a New Triazole Derivative, Is Based on Its Selective Binding Affinity to Cytochrome P450 of Fungi

2002 ◽  
Vol 46 (2) ◽  
pp. 308-314 ◽  
Author(s):  
Masaru Matsumoto ◽  
Kazuya Ishida ◽  
Akihiro Konagai ◽  
Kazunori Maebashi ◽  
Takemitsu Asaoka
Keyword(s):  
Cytochrome P450 ◽  
Antifungal Activity ◽  
In Vivo Studies ◽  
Dependent Manner ◽  
Strong Affinity ◽  
Dose Dependent ◽  
Potent Inhibitory Activity ◽  
In Vitro Antifungal Activity

ABSTRACT SS750 [(R)-(−)-2-(2,4-difluorophenyl)-1-(ethylsulfonyl)-1,1-difluoro-3-(1H-1,2,4-triazol-1-yl)-2-propanol] is a new triazole, and its potential as an antifungal agent was evaluated by in vitro and in vivo studies. In a comparison of the MICs at which 50% of isolates are inhibited (MIC50s) for all strains of Candida species and Cryptococcus neoformans tested, SS750 was four times or more active than fluconazole and had activity comparable to that of itraconazole. The most important advantage of SS750 was that, when the MIC90s were compared, SS750 had 64 and 32 times greater antifungal activities than fluconazole against Candida krusei and Candida glabrata, respectively, which are intrinsically less susceptible to fluconazole. In cyclophosphamide-immunosuppressed mouse models of systemic and pulmonary candidiasis caused by C. albicans, oral SS750 prolonged the number of days of survival of infected animals in a dose-dependent manner and was 4 and ≥64 times more potent than fluconazole and itraconazole, respectively. In a safety profile, SS750, like fluconazole, had less of an affinity for binding to mammalian cytochrome P450 compared with that of ketoconazole, despite its strong affinity for binding to fungal cytochrome P450. The mechanism for the increased in vitro antifungal activity of SS750 against C. krusei is partially due to the potent inhibitory activity (3.7 times versus that of fluconazole) of C. krusei cytochrome P450 sterol 14α-demethylase; SS750 showed a strong affinity for binding to cytochrome P450 of C. krusei, indicating that SS750 acts by inhibiting the cytochrome P450 sterol 14α-demethylase of fungal cells.

In vitro antifungal activity of some South African medicinal plants

2002 ◽  
Vol 68 (1) ◽  
pp. 72-76 ◽  
Author(s):  
A.J. Afolayan ◽  
D.S. Grierson ◽  
L. Kambizi ◽  
I. Madamombe ◽  
P.J. Masika ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Medicinal Plants ◽  
South African ◽  
In Vitro Antifungal Activity

scholarly journals EFEKTIVITAS SENYAWA NONATSIRI DARI Curcuma spp. TERHADAP PENEKANAN PENYAKIT ANTRAKNOSA PADA BUAH CABAI

2020 ◽  
Vol 31 (1) ◽  
pp. 21
Author(s):  
Anella Retna Kumala Sari ◽  
Firdaus Auliya Rahmah ◽  
Syamsuddin Djauhari
Keyword(s):  
Curcuma Longa ◽  
Fungal Growth ◽  
In Vitro Test ◽  
Test Results ◽  
In Vivo Test ◽  
Randomized Design ◽  
Vitro Test ◽  
Rotary Vacuum

<em></em><em>One of the important diseases on chili is anthracnose caused by </em>Colletotrichum capsici<em>. </em>Curcuma<em> extracts and </em><em>their essential oils were known as antifungal, but nonessential compounds have not been widely tested. This study aimed to assay the effectiveness of nonessential compounds of </em>Curcuma longa<em>, </em>C. zedoaria<em>, and </em>C. aeruginosa<em> to </em>C. annuum<em>. This study was conducted in November 2014 until Mei 2015 at Brawijaya University. The nonessential compound was obtained by soaking rhizome of </em>C. longa,   C.   zedoaria<em>,  and </em>C.   aeruginosa<em> in methanol, then distilled by</em><em>using rotary vacuum evaporator. Nonessential chemical compunds were identified by using HPLC. Effectiveness evaluation of nonessential compounds from three species of </em>Curcuma<em> was done by in </em>vitro<em> and </em>in vivo<em> test. Tested treatments were three species of </em>Curcuma<em> spp and 6 concentration levels of nonessential compounds (0 ppm, 4 ppm, 6 ppm, 8 ppm, 10 ppm, and</em><em> 12 ppm). The xperiment was performed in Factorial Complete Randomized Design, with 18 treatments combination, and replicated three times. Results of HPLC analysis showed the rhizomes of the three </em>Curcuma<em> species contained curcumin and </em>desmethoxycurcumin<em> in various concentrations. The highest </em><em>level was found in the </em>C. longa<em> extract (13.792 ppm curcumin and 67.156 ppm </em>desmethoxycurcumin<em>). However, in vitro test results showed nonessential compound of </em>C. zedoaria<em> was most effective in inhibiting </em>C. annuum<em> growth.  The 10 ppm concentration inhibited 81.53 % of fungal growth.  Further, the in vivo test, also indicated the same, it’s most effective in hampering the growth of anthracnose symptoms. Therefore, curcumin and </em>desmethoxycurcumin<em> from three species of </em>Curcuma<em> have potential to be developed as botanical fungicide.</em>

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