Effects of different treatments on seed germination and breaking seed dormancy of Prosopis koelziana and Prosopis Juliflora

2011 ◽  
Vol 22 (1) ◽  
pp. 35-38 ◽  
Author(s):  
Salman Zare ◽  
Ali Tavili ◽  
Mohammad Javad Darini
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Prosopis Juliflora ◽  
Breaking Seed Dormancy

scholarly journals Practical Methods for Breaking Seed Dormancy in a Wild Ornamental Tulip Species Tulipa thianschanica Regel

Agronomy ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1765
Author(s):  
Wei Zhang ◽  
Lian-Wei Qu ◽  
Jun Zhao ◽  
Li Xue ◽  
Han-Ping Dai ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Combined Treatment ◽  
Individual Treatment ◽  
Cold Stratification ◽  
Slight Effect ◽  
Sowing Depth ◽  
Physiological Dormancy ◽  
Commercial Breeding ◽  
Breaking Seed Dormancy

The innate physiological dormancy of Tulipa thianschanica seeds ensures its survival and regeneration in the natural environment. However, the low percentage of germination restricts the establishment of its population and commercial breeding. To develop effective ways to break dormancy and improve germination, some important factors of seed germination of T. thianschanica were tested, including temperature, gibberellin (GA3) and/or kinetin (KT), cold stratification and sowing depth. The percentage of germination was as high as 80.7% at a constant temperature of 4 °C, followed by 55.6% at a fluctuating temperature of 4/16 °C, and almost no seeds germinated at 16 °C, 20 °C and 16/20 °C. Treatment with exogenous GA3 significantly improved the germination of seeds, but KT had a slight effect on the germination of T. thianschanica seeds. The combined treatment of GA3 and KT was more effective at enhancing seed germination than any individual treatment, and the optimal hormone concentration for the germination of T. thianschanica seeds was 100 mg/L GA3 + 10 mg/L KT. In addition, it took at least 20 days of cold stratification to break the seed dormancy of T. thianschanica. The emergence of T. thianschanica seedlings was the highest with 82.4% at a sowing depth of 1.5 cm, and it decreased significantly at a depth of >3.0 cm. This study provides information on methods to break dormancy and promote the germination of T. thianschanica seeds.

scholarly journals Physiology of Breaking Seed Dormancy and Increasing Seed Germination in Senna alata (L) Roxb Seeds in Nigeria

2020 ◽  
Vol 15 (1) ◽  
pp. 1-6
Author(s):  
Vincent Ishola Esa ◽  
Taiwo Ayanniyin Ayanbamiji ◽  
Ayobami Daniel Abo
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Breaking Seed Dormancy ◽  
Senna Alata

scholarly journals Methods of breaking seed dormancy for ornamental passion fruit species

2017 ◽  
Vol 23 (1) ◽  
pp. 72 ◽  
Author(s):  
Thalita Neves Marostega ◽  
Petterson Baptista Da Luz ◽  
Armando Reis Tavares ◽  
Leonarda Grillo Neves ◽  
Severino De Paiva Sobrinho
Keyword(s):  
Experimental Design ◽  
Seed Germination ◽  
Gibberellic Acid ◽  
Seed Dormancy ◽  
Potassium Nitrate ◽  
Plant Production ◽  
Commercial Plant ◽  
Fruit Species ◽  
Breaking Seed Dormancy ◽  
And Control

The Passiflora L. genus covers a diversity of wild species with ornamental potential, especially due to the intrinsic beauty of its exotic flowers, flowering more than once a year and the lush foliage. However, Passiflora seeds present dormancy complicating seed germination and the establishment of commercial plant production with species with high ornamental potential. This study was conducted to determine the best pre-germination treatments to overcome seed dormancy for Passiflora quadrangularis, P. nitida, P. foetida, P. eichleriana, P. alata, P. cincinnata, P. mucronata, P. micropetala, P. suberosa, P. morifolia and P. tenuifila. The experimental design was completely randomized, with five treatments and four replicates, with 25 seeds per plot. Pre-germination treatments were: seeds soaked in 1,000 mg L- 1 GA3 (gibberellic acid) for 6 hours, seeds soaked in 0.2 % KNO3 (potassium nitrate) for 24 hours, seeds soaked in 1 % KNO3 for 24 hours, partial seedcoat scarification with sandpaper number 120 and control (seeds untreated). Percentage of germination, germination velocity index and radicle length were evaluated for all species. The results showed that GA3 was effective to overcome seed dormancy in P. suberosa (86%), P. morifolia (68 %) and P. tenuifila (54%). KNO3 1% had significant effect on overcoming dormancy in seeds of P. eichleriana (66%) and scarification with sandpaper increased seed germination of P. micropetala (38%).

scholarly journals The Effect of Sodium Hypochlorite (NaOCl) and Heat Treatments on Seed Germination of Rice: An Approach to Restore Seed Viability

2021 ◽  
pp. 49-53
Author(s):  
Monoj Sutradhar ◽  
Subhasis Samanta ◽  
Brijesh Kumar Singh ◽  
Md. Nasim Ali ◽  
Nirmal Mandal
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Sodium Hypochlorite ◽  
Seed Viability ◽  
Germination Percentage ◽  
Heat Treated ◽  
Mother Plants ◽  
Breaking Seed Dormancy ◽  
One Year

Dormancy in rice serves as a mechanism of survival by protecting the seed from germinating in the mother plants; however, it becomes a problem in germination during sowing in soil or under in vitro conditions. This study was conducted to determine the effect of heat treatment and sodium hypochlorite (NaOCl) treatment of seeds on dormancy alleviation. The seeds included both freshly harvested seeds and one-year-old stored seeds, which were tested for germination after different types of seed treatments. Both the treatments increased the germination percentage in seeds, however, it was lesser in the case of old seeds. The best results were obtained from 2% NaOCl treatment for 24 hrs in new seeds, i.e. 92.84±0.103 % germination percentage (GP). However, the higher GP in old seeds were obtained from 48 hrs of heat-treated seeds i.e. 82.9±0.509 % GP. The results of the experiment revealed that rice seeds start to lose viability within a year due to seed dormancy, but this can be reversed with proper measures. These methods of breaking seed dormancy can be considered effective to break seed dormancy and improve seed germination in rice.

Population ecology of Cirsium pitcheri on Lake Huron sand dunes. III. Mechanisms of seed dormancy

1998 ◽  
Vol 76 (4) ◽  
pp. 575-586 ◽  
Author(s):  
Hua Chen ◽  
M A Maun
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Seed Coat ◽  
Threatened Species ◽  
Sand Dunes ◽  
Growth Chamber ◽  
Oenothera Biennis ◽  
Breaking Seed Dormancy ◽  
Cirsium Pitcheri ◽  
Germination Requirements

Growth chamber studies were conducted to examine seed dormancy and germination requirements of Pitcher's thistle (Cirsium pitcheri (Torr. ex Eaton) Torr. & Gray), a threatened species endemic to the shoreline sand dunes of the Great Lakes. We determined the effects of different environmental regimes on breaking seed dormancy of this monocarpic perennial. The data showed that seeds of C. pitcheri possessed innate dormancy that was caused by a hard seed coat as well as inhibitory compounds within the seed. Seed germination requirements were very specific. Exposure of seeds to different temperatures and photoperiods in a growth chamber had little effect on breaking seed dormancy unless the seeds had been pretreated. Pretreatment of seeds by cold stratification and its duration, scarification by sand paper or sulphuric acid, and application of gibberellic acid were also not very effective for breaking dormancy of C. pitcheri. High germination was obtained only when seeds were pretreated either by surgically removing the seed coat or by nicking the seed on the radicle or cotyledonary end. After this pretreatment seeds germinated over a temperature range of 15-30°C, but the highest proportion of seeds germinated when temperatures were above 20°C. Under natural conditions, germination of C. pitcheri seeds occurs in spring after they have overwintered and experienced the pretreatment of stratification and scarification through freezing and thawing of the substrate. Aqueous extract of C. pitcheri seeds had a strong inhibitory effect on germination of Oenothera biennis L. seeds indicating an allelopathic chemical in the seed. There was no relationship between the seed size of C. pitcheri and the germinability of seeds.Key words: Cirsium pitcheri, threatened species, seed dormancy, seed germination, scarification, stratification, nicking of seeds.

scholarly journals Comparative metabolites profiling of Polygonatum cyrtonema Hua seed during sand storage and germination

2020 ◽  
Author(s):  
Qing Jin ◽  
Jinfeng Tong ◽  
Wenwu Zhang ◽  
Long Xia ◽  
Xiaoyun Zhu ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Seed Coat ◽  
Metabolic Pathways ◽  
Vital Role ◽  
Metabolic Pathway Analysis ◽  
Germination Process ◽  
Breaking Seed Dormancy ◽  
Metabolic Activities ◽  
Storage Times

Abstract Background: The seeds of Polygonatum cyrtonema Hua have dormancy phenomenon. Previous studies have shown that sand storage factors effects of the seed dormancy of P. cyrtonema Hua seeds and enhance the seed germination process. Subsequently, metabolic activities and different changes during the sand storage and germination process of P. cyrtonema Hua seed has not been heavily researched.Results: In this study the changes in the metabolites of P. cyrtonema Hua seeds at different sand storage times and germination stages, we used untargeted metabolomics to determine them. Most of the sugar and glycoside contents in seed coat increased after 30 d on the other hand, in peeled seeds increased at 30 d and decreased at 60 d after sand storage treatment. The content of proline and benzoic acid decreased in the seed coat after sand storage. PCA, OPLS-DA and HCA showed that the contents of most metabolites increased after 7 d and decreased after 14 d of seed germination. The process of 7 d to 14 d was the key stage of seed germination of P. cyrtonema Hua. Differential metabolic pathway analysis showed that seed germination was controlled by multiple metabolic pathways. Metabolic correlation revealed the interdependence between seed germination metabolites and metabolic pathways. Conclusion: Sand storage can significantly increase the rate of seed germination and play a vital role in seed dormancy of P. cyrtonema Hua. There was inherent differences in metabolites during different storage time and germination stages in P. cyrtonema Hua. Our work provides a first glimpse of the metabolome in seed germination of P. cyrtonema Hua, and provides a valuable informations for revealing the mechanism of breaking seed dormancy.

scholarly journals Effect of different pre-treatments on seed germination of Prosopis juliflora and Dalbergia sissoo: a step towards mutation breeding

2020 ◽  
Vol 66 (No. 2) ◽  
pp. 80-87
Author(s):  
Asif Javed Muhammad
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Gamma Ray ◽  
Mutation Breeding ◽  
Prosopis Juliflora ◽  
Breeding Program ◽  
Boiling Water ◽  
Dalbergia Sissoo ◽  
Tree Form ◽  
Positive Effects

For improving the seed germination of Prosopis juliflora and Dalbergia sissoo different treatments were tested, including side cutting, abrasion, overnight soaking in boiling water, scalding in actively boiling water for <br />1 minute and immersion in 30%, 60% and 95% H<sub>2</sub>SO<sub>4</sub> solution. Results showed that abrasion with sandpaper and side cutting were the most effective methods to break seed dormancy in both species, while scalding in actively boiling water for 1 minute, overnight soaking and different concentrations of H<sub>2</sub>SO<sub>4</sub> gave low to zero seed germination. Based on the positive effects of scarification it was concluded that seed dormancy in both species was due to water impermeability of the seed coat. Mutation breeding involves the treatment of large quantities of seeds, therefore abrasion with sandpaper was the most efficient and less labour-intensive method; this method was subsequently used for determination of LD<sub>50</sub> as it is a prerequisite in a mutation breeding program. Abrasion before irradiation had a positive effect on P. juliflora whereas it had a negative effect on D. sissoo seeds. Seeds of both species were exposed to different doses of gamma rays such as 0, 100, 200, 300, 400 and 500 Gy using a <sup>60</sup>Co source. The LD<sub>50</sub> for P. juliflora was 651 Gy based on the rate of seed germination indicating that P. juliflora had tolerance to irradiation and low radiosensitivity to gamma ray. A high LD<sub>50</sub> of 1097 Gy was observed for D. sissoo, suggesting high tolerance to irradiation and very low radio sensitivity. These findings will help to initiate a mutation breeding program in both species to obtain desirable mutants with desirable characteristics such as thornless genotypes, better tree form, disease resistance and increased genetic diversity.

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