Coproduction of polyhydroxyalkanoates and exopolysaccharide by submerged fermentation using autochthonous bacterial strains

2022 ◽  
Author(s):  
José A. Vega-Vidaurri ◽  
Francisco Hernández-Rosas ◽  
María A. Ríos-Corripio ◽  
Juan M. Loeza-Corte ◽  
Marlon Rojas-López ◽  
...  
Keyword(s):  
Submerged Fermentation ◽  
Bacterial Strains

scholarly journals Amylase Production by Bacillus subtilis SY134D Strain Under Submerged Fermentation

2020 ◽  
Vol 63 (2) ◽  
pp. 113-118
Author(s):  
Yasser Bakri ◽  
Samir Elkhouri ◽  
Muhammad Harba ◽  
Yasser Akeed
Keyword(s):  
Bacillus Subtilis ◽  
Submerged Fermentation ◽  
Oxygen Transfer Rate ◽  
Product Formation ◽  
Aeration Rate ◽  
Agitation Speed ◽  
Bacterial Strains ◽  
Amylase Production ◽  
Soybean Cake ◽  
Fermentation Technology

Industrial important enzymes have traditionally been obtained by using submerged fermentation technology. Many organisms are able to produce these enzymes, but only a few of them exhibit satisfactory characteristics for industrial application. The ability of bacterial strains from the genus Bacillus to secrete large amounts of extracellular a-amylase has made them well suited for commercial production. Amylase production using Bacillus subtilis SY134D strain was conducted in flasks and bioreactor. In flasks, our results showed that soybean cake, beet pomace and tomato pomace were the best carbon source used in submerged fermentation (SmF) after 72 h of incubation. In bioreactor, the rates of air flow and agitation speed are important factors that affect oxygen transfer rate which influence on product formation. When the aeration rate was increased from 0.25 to 0.75 vvm, amylase production increased at the speed of agitation 100 and 200 rpm. The best enzyme yield 127 IU/mL was obtained at 0.25 vvm an aeration rate and 300 rpm agitation speed in a 3L Electro-lab bio-reactor.      

scholarly journals ISOLATION OF CELLULOLYTIC BACTERIA FROM SOIL AND VALORIZATION OF DIFFERENT LIGNOCELLULOSIC WASTES FOR CELLULASE PRODUCTION BY SUBMERGED FERMENTATION

2021 ◽  
Vol 55 (7-8) ◽  
pp. 821-828
Author(s):  
MISBAH GHAZANFAR ◽  
MUHAMMAD IRFAN ◽  
MUHAMMAD NADEEM ◽  
HAFIZ ABDULLAH SHAKIR ◽  
MUHAMMAD KHAN ◽  
...  
Keyword(s):  
Bacillus Megaterium ◽  
Submerged Fermentation ◽  
Pseudomonas Stutzeri ◽  
Cellulase Production ◽  
Rrna Gene ◽  
Cellulolytic Bacteria ◽  
Bacterial Strains ◽  
Eucalyptus Leaves ◽  
Lignocellulosic Wastes ◽  
Bacillus Flexus

Cellulases are known to convert cellulose into monomeric or dimeric structures, hence playing an important role in bioethanol production, along with their applications in textile and paper industries. This study was directed towards the isolation and screening of cellulase producing bacteria from different soil samples on CMC (carboxymethyl cellulose) agar plates, followed by Gram’s iodine staining. Six strains showed clear zones of hydrolysis on CMC agar plates. Isolates were identified as Bacillus megaterium, Pseudomonas stutzeri, Bacillus aerius, Bacillus paralichniformis, Bacillus flexus, and Bacillus wiedmanni by 16S rRNA gene sequencing. These strains were cultivated by submerged fermentation for cellulase production using various lignocellulosic wastes, such as corn cob, rice husk, wheat straw, seed pods of Bombax ceiba and eucalyptus leaves. Results showed that Pseudomonas stutzeri is the best cellulase producer among these strains. It offered the highest cellulase activity of 170.9±4.1 (IU/mL/min) in media containing eucalyptus leaves after 24 h of incubation at 37 °C, followed by Bacillus paralichniformis, Bacillus wiedmanni, Bacillus flexus, Bacillus aerius and Bacillus megaterium. These bacterial strains and lignocellulosic wastes could be potentially used for industrial exploitation, particularly in biofuels and textiles.

SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL EVALUATION OF 3-FORMYL INDOLE BASED SCHIFF BASES

2018 ◽  
Vol 7 (6) ◽  
Author(s):  
Singh Gurvinder ◽  
Singh Prabhsimran ◽  
Dhawan R. K.
Keyword(s):  
Antimicrobial Activity ◽  
Spectral Analysis ◽  
Schiff Bases ◽  
Antimicrobial Agents ◽  
Biological Evaluation ◽  
Fungal Strain ◽  
Bacterial Strains ◽  
Standard Drug ◽  
Gram Negative ◽  
E Coli

In order to develop new antimicrobial agents, a series of 3-formyl indole based Schiff bases were synthesized by reacting 3-formyl indole(indole-3-carboxaldehyde) with substituted aniline taking ethanol as solvent. The reaction was carried in the presence of small amount of p-toluene sulphonic acid as catalyst.All the synthesized compounds were characterized by IR, 1H-NMR spectral analysis. All the synthesized compounds were evaluated for antimicrobial activity against two gram positive bacterial strains (B. subtilisand S. aureus) and two gram negative bacterial strains (P. aeruginosaand E. coli) and one fungal strain (C. albicans). All the synthesized compounds were found to have moderate to good antimicrobial activity. The  standard drug amoxicillin, fluconazole were used for antimicrobial activity. Among the synthesized compounds, the maximum antimicrobial activity was shown by compounds GS04, GS07, GS08 and GS10.

THE EFFECT OF BACTERIAL STRAINS ON THE ENZYMATIC ACTIVITY OF SOILS IN IMPACT ZONE OF ATAMAN LAKE

2020 ◽  
Author(s):  
V.V. Zinchenko ◽  
◽  
E.S Fedorenko ◽  
A.V Gorovtsov ◽  
T.M Minkina ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Dehydrogenase Activity ◽  
Contaminated Soil ◽  
Model Experiment ◽  
Impact Zone ◽  
Bacterial Strains ◽  
The Impact

As a result of the model experiment, an increase in the enzymatic activity of meadow chernozem of the impact zone of Ataman Lake with the introduction of a strains mixture of metal-resistant microorganisms into the soil was established. The experiment has shown that the application of bacterial strains increases the dehydrogenase activity of contaminated soil by 51.8% compared to the variant without remediation

Preliminary in Vitro Investigations on The Inhibitory Activity of The Original Dietary Supplement Oxidal® on Pathogenic Bacterial Strains

2020 ◽  
Vol 11 ◽  
pp. 37-43
Author(s):  
Prof. Teodora P. Popova ◽  
Toshka Petrova ◽  
Ignat Ignatov ◽  
Stoil Karadzhov
Keyword(s):  
Dietary Supplement ◽  
Broad Spectrum ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Clinical Effectiveness ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Microbial Strains

The antimicrobial action of the dietary supplement Oxidal® was tested using the classic Bauer and Kirby agar-gel diffusion method. Clinical and reference strains of Staphylococcus aureus and Escherichia coli were used in the studies. The tested dietary supplement showed a well-pronounced inhibitory effect against the microbial strains commensurable with that of the broad-spectrum chemotherapeutic agent Enrofloxacin and showed even higher activity than the broad spectrum antibiotic Thiamphenicol. The proven inhibitory effect of the tested dietary supplement against the examined pathogenic bacteria is in accordance with the established clinical effectiveness standards for antimicrobial agents.

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