The influence of the cooling of donor cultures on the in vitro adventitious regeneration and carbohydrate metabolism of four dwarfing apple rootstocks

2009 ◽  
Vol 32 (2) ◽  
pp. 333-340 ◽  
Author(s):  
Teresa Orlikowska ◽  
Marta Zawadzka ◽  
Danuta Kucharska ◽  
Lesław B. Lahuta
Keyword(s):  
Carbohydrate Metabolism ◽  
Apple Rootstocks ◽  
Adventitious Regeneration

Insulin-like action of proinsulin on rat liver carbohydrate metabolism in vitro

Diabetes ◽  
1985 ◽  
Vol 34 (5) ◽  
pp. 415-419 ◽  
Author(s):  
I. Probst ◽  
H. Hartmann ◽  
K. Jungermann ◽  
W. Creutzfeldt
Keyword(s):  
Carbohydrate Metabolism ◽  
Rat Liver

scholarly journals The in vitro manipulation of carbohydrate metabolism: a new strategy for deciphering the cellular defence mechanisms against nitric oxide attack

1999 ◽  
Vol 344 (3) ◽  
pp. 643 ◽  
Author(s):  
Claire LE GOFFE ◽  
Geneviève VALLETTE ◽  
Anne JARRY ◽  
Chantal BOU-HANNA ◽  
Christian L. LABOISSE
Keyword(s):  
Nitric Oxide ◽  
Carbohydrate Metabolism ◽  
Defence Mechanisms ◽  
New Strategy ◽  
Cellular Defence

Congenital Dyserythropoietic Anemia

PEDIATRICS ◽  
1973 ◽  
Vol 51 (5) ◽  
pp. 957-958
Author(s):  
G. Bennett Humphrey ◽  
Bahaod-Din Mojab ◽  
Ingomar Mutz
Keyword(s):  
Carbohydrate Metabolism ◽  
Morphological Characteristics ◽  
Type I ◽  
Type Ii ◽  
Congenital Dyserythropoietic Anemia ◽  
The 1950S ◽  
Deja Vu ◽  
Déjà Vu ◽  
Excellent Article

Reading the excellent article by Drs. Murphy and Oski, "Congenital Dyserythropoietic Anemia (CDA)",1 which further defines type II, produced a sense of deja vu. In the 1950s, nonspherocytic, hemolytic anemias (HNHA) were categorized as type I and II based on the in vitro autohemolysis test.2 This group of anemias has subsequently been demonstrated to be due to a series of enzymatic abnormalities in carbohydrate metabolism.3 In CDA, the morphological characteristics which define types I, II, and III probably reflect nuclear rather than cytoplasmic abnormalities.

Temporal changes in glucose and carbohydrate metabolism in the oncospheres of the cyclophyllidean tapeworm, Hymenolepis diminuta

Parasitology ◽  
1993 ◽  
Vol 106 (3) ◽  
pp. 317-325 ◽  
Author(s):  
P. W. Pappas ◽  
G. M. Durka
Keyword(s):  
Molecular Weight ◽  
Glucose Metabolism ◽  
Carbohydrate Metabolism ◽  
Glucose Absorption ◽  
Temporal Changes ◽  
Hymenolepis Diminuta ◽  
Temporal Shift ◽  
Carbohydrate Fraction ◽  
Ethanol Extracts

SUMMARYWhen incubated in vitro for 24 h, oncospheres of Hymenolepis diminuta absorb and metabolize radioactive glucose. Between 0 and 12 h post-activation, oncospheres absorb glucose, but glucose is neither metabolized into other carbohydrates nor incorporated into the ethanol-precipitable fraction (which would contain glycogen). Between 12 and 24 h post-activation glucose is incorporated into a number of higher molecular weight carbohydrates that are demonstrable in ethanol extracts of the larvae, as well as the incubation media. Furthermore, measurable amounts of radioactivity are incorporated into the ethanol-precipitable carbohydrate fraction of oncospheres. To determine if these temporal changes in carbohydrate metabolism occurred spontaneously following activation, oncospheres were pre-incubated for 12 h (0–12 h post-activation) in the absence or presence of glucose, and then transferred to media containing radioactive glucose for an additional 12 h (12–24 h post-activation). In these latter experiments, glucose absorption and metabolism between 12 and 24 h post-activation were virtually identical to glucose metabolism in oncospheres that were incubated in radioactive glucose for 0–12 h immediately following activation. Thus, these data do not support the hypothesis that the temporal shift in carbohydrate metabolism occurs spontaneously.

Carbohydrate Metabolism of Chick Fibroblasts in Vitro

1942 ◽  
Vol 18 (3) ◽  
pp. 237-256
Author(s):  
E. N. WILLMER
Keyword(s):  
Lactic Acid ◽  
Carbohydrate Metabolism ◽  
Acid Production ◽  
Lactic Acid Production ◽  
High Growth ◽  
Glucose Consumption ◽  
High Growth Rate ◽  
Absolute Correlation ◽  
Heart Cultures

1. The glucose consumption of osteoblasts growing in a medium of plasma and embryo juice has been compared with that of the same cells in plasma and Tyrode medium. Embryo juice causes an initial increased glucose consumption which later falls below the level characteristic of a Tyrode-plasma medium. 2. A modification of a method of measuring the nucleoprotein phosphorus content of tissue cultures is described and among other data obtained the changes which occur in this figure have been noted for fourth passage chick osteoblast cultures and for fresh heart explants during the first 4 days of culture in media of plasma and Tyrode and of plasma and embryo juice. 3. The figures for nucleoprotein content have been correlated with those for glucose consumption and lactic acid production and pronounced differences have been observed in the behaviour of the two types of tissue. The possible significance of the findings is discussed. 4. There is no absolute correlation between high growth rate and high sugar consumption or lactic acid production. 5. The data discussed are consistent with the idea that an increased protein metabolism, and a decreased carbohydrate metabolism result from the addition of embryo juice to osteoblast cultures. Embryo juice has no detectable effects on the carbohydrate metabolism of fresh heart cultures.

scholarly journals Embryo rescue from interspecific crosses in apple rootstocks

2006 ◽  
Vol 41 (6) ◽  
pp. 969-973 ◽  
Author(s):  
Adriana Cibele de Mesquita Dantas ◽  
José Itamar Boneti ◽  
Rubens Onofre Nodari ◽  
Miguel Pedro Guerra
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Embryo Rescue ◽  
Casein Hydrolysate ◽  
Female Parent ◽  
Interspecific Crosses ◽  
Open Pollination ◽  
Apple Rootstocks ◽  
Hand Pollination

The objetive of this work was to rescue immature embryos of apple rootstocks Malus prunifolia (Marubakaido) and Malus pumila (M9) after 40-60 days of pollination and to put them into MS culture media supplemented with agar (6 g L-1) and casein hydrolysate (500 mg L-1). Embryos originated from interspecific crosses and open pollination showed differences in the in vitro responses, depending on the female parent, the developmental stage of the embryo, and the culture medium composition. Embryos of the M. pumila rootstock, rescued within 40 days after pollination and put in culture medium supplemented with indolacetic acid (IAA), gibberellic acid (GA3), kinetin and maltose, resulted in a normal development of plantlets. However, embryos originating from hand-pollination, cultivated in medium supplemented with 14 µM IAA, 5 µM kinetin and 1.5 µM Ga3 (MS1), mainly those of M. prunifolia x M. pumila, showed a high percentage of rusted embryos (96.2%). Embryos from open pollination of M. prunifolia and M. pumila formed calluses. It was possible to identify the influence of the female parent by the enhanced development of M. pumila shoots derived from open or hand-pollination. The crossing of responsive species and the use of the technique of embryo culture provided a rapid and uniform germination and, consequently, the development of fully normal seedlings.

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