Simultaneous removal of Cr(VI) and phenol from synthetic binary solution using consortium culture of Bacillus sp. and E. coli immobilized on tea waste biomass in packed bed reactor

2015 ◽  
Vol 33 (2) ◽  
pp. 559-566 ◽  
Author(s):  
Ankur Gupta ◽  
Chandrajit Balomajumder
2008 ◽  
Vol 18 (4) ◽  
pp. 1014-1020 ◽  
Author(s):  
Yun-guo LIU ◽  
Cui PAN ◽  
Wen-bin XIA ◽  
Guang-ming ZENG ◽  
Ming ZHOU ◽  
...  

2010 ◽  
Vol 101 (3) ◽  
pp. 853-858 ◽  
Author(s):  
Mathur Nadarajan Kathiravan ◽  
Ravikumar Karthiga Rani ◽  
Ramalingam Karthick ◽  
Karuppan Muthukumar

2019 ◽  
Vol 6 (4) ◽  
pp. 182035 ◽  
Author(s):  
Licheng Wu ◽  
Xiaolei Guo ◽  
Gaobing Wu ◽  
Pengfu Liu ◽  
Ziduo Liu

α-keto acids are compounds of primary interest for the fine chemical, pharmaceutical and agrochemical sectors. l -amino acid oxidases as an efficient tool are used for α-keto acids preparation in this study. Firstly, an l -amino acid oxidase ( Pmi LAAO) from Proteus mirabilis was discovered by data mining . Secondly, by gene expression vector screening, pETDuet-1- Pmi LAAO activity improved by 130%, as compared to the pET20b- Pmi LAAO. Pmi LAAO production was increased to 9.8 U ml −1 by optimized expression condition (OD 600 = 0.65, 0.45 mmol l −1 IPTG, 20 h of induction). Furthermore, The Pmi LAAO was stabile in the pH range of 4.0–9.0 and in the temperature range of 10–40°C; the optimal pH and temperature of recombinant Pmi LAAO were 6.5 and 37°C, respectively. Afterwards, in order to simplify product separation process, E. coli -pETduet-1- Pmi LAAO was immobilized in Ca-alginate beads. Continuous production of 2-oxo-3-phenylpropanoic acid was conducted in a packed-bed reactor via immobilized E. coli -pETduet-1- Pmi LAAO. Significantly, 29.66 g l −1 2-oxo-3-phenylpropanoic acid with a substrate conversion rate of 99.5% was achieved by correspondingly increasing the residence time (25 h). This method holds the potential to be used for efficiently producing pure α-keto acids.


1990 ◽  
Vol 589 (1 Biochemical E) ◽  
pp. 540-552 ◽  
Author(s):  
HIROYASU SEKO ◽  
SHINOBU TAKEUCHI ◽  
KAZUYOSHI YAJIMA ◽  
MASARU SENUMA ◽  
TETSUYA TOSA

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