Microscopic Study of the Phase Transformation During the Oxygen-Enriched Direct Smelting of Jamesonite Concentrate

Herpes-like viruses were isolated from rhesus monkey peripheral blood leucocytes when co-cultivated with WI-38 cells. The virus was originally designated rhesus leucocyte-associated herpesvirus (LAHV) and subsequently called Herpesvirus mulatta (HVM). The original isolations were from juvenile rhesus monkeys shown to be free of antibody to rhesus cytomegalic virus. The virus could only be propagated in human or simian fibroblasts. Use of specific antisera developed from HVM showed no relationship between this virus and other herpesviruses. An electron microscopic study was undertaken to determine the morphology of Herpesvirus mulatta (HVM) in infected human fibroblasts.

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Ultrastructural Investigation of Spontaneous Pituitary Adenomas in Aging Sprague-Dawley Rats

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100053747 ◽

1982 ◽

Vol 40 ◽

pp. 216-217

Author(s):

D. J. McComb ◽

J. Beri ◽

F. Zak ◽

K. Kovacs

Keyword(s):

Microscopic Study ◽

Pituitary Adenomas ◽

Wistar Rats ◽

Microscopic Level ◽

Sprague Dawley ◽

Prolactin Cells ◽

Light Microscopic Level ◽

Ultrastructural Investigation ◽

Sprague Dawley Rats ◽

Long Evans

Investigation of the spontaneous pituitary adenomas in rat have been limited mainly to light microscopic study. Furth et al. (1973) described them as chromophobic, secreting prolactin. Kovacs et al. (1977) in an ul trastructural investigation of adenomas of old female Long-Evans rats, found that they were composed of prolactin cells. Berkvens et al. (1980) using immunocytochemistry at the light microscopic level, demonstrated that some spontaneous tumors of old Wistar rats could contain GH, TSH or ACTH as well as PRL.

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Further Observations on the Virus of Epizootic Diarrhea of Infant Mice. An Electron Microscopic Study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100060568 ◽

1967 ◽

Vol 25 ◽

pp. 110-111

Author(s):

W. G. Banfield ◽

G. Kasnic ◽

J. H. Blackwell

Keyword(s):

Electron Microscope ◽

Infected Cell ◽

Microscopic Study ◽

Intestinal Epithelium ◽

Ultrastructural Study ◽

Osmium Tetroxide ◽

Lead Citrate ◽

Electron Microscopic ◽

Virus Particles ◽

Infected Cells

An ultrastructural study of the intestinal epithelium of mice infected with the agent of epizootic diarrhea of infant mice (EDIM virus) was first performed by Adams and Kraft. We have extended their observations and have found developmental forms of the virus and associated structures not reported by them.Three-day-old NLM strain mice were infected with EDIM virus and killed 48 to 168 hours later. Specimens of bowel were fixed in glutaraldehyde, post fixed in osmium tetroxide and embedded in epon. Sections were stained with uranyl magnesium acetate followed by lead citrate and examined in an updated RCA EMU-3F electron microscope.The cells containing virus particles (infected) are at the tips of the villi and occur throughout the intestine from duodenum through colon. All developmental forms of the virus are present from 48 to 168 hours after infection. Figure 1 is of cells without virus particles and figure 2 is of an infected cell. The nucleus and cytoplasm of the infected cells appear clearer than the cells without virus particles.

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Cell Wall Ultrastructure in Three Strains of a Cariogenic Streptococcus

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100065730 ◽

1971 ◽

Vol 29 ◽

pp. 338-339

Author(s):

M. J. Kramer ◽

Alan L. Coykendall

Keyword(s):

Cell Wall ◽

Dental Caries ◽

Streptococcus Mutans ◽

Microscopic Study ◽

Electron Microscopic Study ◽

Genetic Heterogeneity ◽

Morphological Characteristics ◽

Electron Microscopic ◽

Dna Reassociation ◽

Wall Ultrastructure

During the almost 50 years since Streptococcus mutans was first suggested as a factor in the etiology of dental caries, a multitude of studies have confirmed the cariogenic potential of this organism. Streptococci have been isolated from human and animal caries on numerous occasions and, with few exceptions, they are not typable by the Lancefield technique but are relatively homogeneous in their biochemical reactions. An analysis of the guanine-cytosine (G-C) composition of the DNA from strains K-1-R, NCTC 10449, and FA-1 by one of us (ALC) revealed significant differences and DNA-DNA reassociation experiments indicated that genetic heterogeneity existed among the three strains. The present electron microscopic study had as its objective the elucidation of any distinguishing morphological characteristics which might further characterize the respective strains.

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Phase Transformation In Ti-6al-4v Alloys

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100096175 ◽

1972 ◽

Vol 30 ◽

pp. 584-585

Author(s):

Shiro Fujishiro

Keyword(s):

Phase Transformation ◽

Grain Boundary ◽

Cryogenic Temperature ◽

Β Phase ◽

Heat Treated ◽

Mixed Microstructure ◽

Ductile Behavior

The Ti-6 wt.% Al-4 wt.% V commercial alloys have exhibited an improved formability at cryogenic temperature when the alloys were heat-treated prior to the tests. The author was interested in further investigating this unusual ductile behavior which may be associated with the strain-induced transformation or twinning of the a phase, enhanced at lower temperatures. The starting materials, supplied by RMI Co., Niles, Ohio were rolled mill products in the form of 40 mil sheets. The microstructure of the as-received materials contained mainly ellipsoidal α grains measuring between 1 and 5μ. The β phase formed an undefined grain boundary around the a grains. The specimens were homogenized at 1050°C for one hour, followed by aging at 500°C for two hours, and then quenched in water to produce the α/β mixed microstructure.

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Microstructural and Microchemical Characterization of Nickel Sulfide Inclusions in Plate Glass

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100089123 ◽

1991 ◽

Vol 49 ◽

pp. 962-963

Author(s):

J. Cooper ◽

O. Popoola ◽

W. M. Kriven

Keyword(s):

Thermal Expansion ◽

Phase Transformation ◽

Glass Matrix ◽

Nickel Sulfide ◽

Plate Glass ◽

Thermal Expansion Mismatch ◽

Volume Increase ◽

Β Phase ◽

Microchemical Characterization

Nickel sulfide inclusions have been implicated in the spontaneous fracture of large windows of tempered plate glass. Two alternative explanations for the fracture-initiating behaviour of these inclusions have been proposed: (1) the volume increase which accompanies the α to β phase transformation in stoichiometric NiS, and (2) the thermal expansion mismatch between the nickel sulfide phases and the glass matrix. The microstructure and microchemistry of the small inclusions (80 to 250 μm spheres), needed to determine the cause of fracture, have not been well characterized hitherto. The aim of this communication is to report a detailed TEM and EDS study of the inclusions.

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