Antimicrobial Activity of Thymol and Thymol-Nanoemulsion Against Three Food-Borne Pathogens Inoculated in a Sausage Model

Author(s):  
Somayeh Sepahvand ◽  
Sedigheh Amiri ◽  
Mohsen Radi ◽  
Hamid-Reza Akhavan
2006 ◽  
Vol 51 (3) ◽  
pp. 912-922 ◽  
Author(s):  
Albert van Dijk ◽  
Edwin J. A. Veldhuizen ◽  
Stefanie I. C. Kalkhove ◽  
Johanna L. M. Tjeerdsma-van Bokhoven ◽  
Roland A. Romijn ◽  
...  

ABSTRACT Food-borne pathogens are responsible for most cases of food poisoning in developed countries and are often associated with poultry products, including chicken. Little is known about the role of β-defensins in the chicken digestive tract and their efficacy. In this study, the expression of chicken β-defensin gallinacin-6 (Gal-6) and its antimicrobial activity against food-borne pathogens were investigated. Reverse transcription-PCR analysis showed high expression of Gal-6 mRNA in the esophagus and crop, moderate expression in the glandular stomach, and low expression throughout the intestinal tract. Putative transcription factor binding sites for nuclear factor kappa beta, activator protein 1, and nuclear factor interleukin-6 were found in the Gal-6 gene upstream region, which suggests a possible inducible nature of the Gal-6 gene. In colony-counting assays, strong bactericidal and fungicidal activity was observed, including bactericidal activity against food-borne pathogens Campylobacter jejuni, Salmonella enterica serovar Typhimurium, Clostridium perfringens, and Escherichia coli. Treatment with 16 μg/ml synthetic Gal-6 resulted in a 3 log unit reduction in Clostridium perfringens survival within 60 min, indicating fast killing kinetics. Transmission electron microscopy examination of synthetic-Gal-6-treated Clostridium perfringens cells showed dose-dependent changes in morphology after 30 min, including intracellular granulation, cytoplasm retraction, irregular septum formation in dividing cells, and cell lysis. The high expression in the proximal digestive tract and broad antimicrobial activity suggest that chicken β-defensin gallinacin-6 plays an important role in chicken innate host defense.


Author(s):  
T Budiati ◽  
W Suryaningsih ◽  
S. Umaroh ◽  
B. Poerwanto ◽  
A Bakri ◽  
...  

2020 ◽  
Vol 36 ◽  
pp. 100635 ◽  
Author(s):  
Yesim Ozogul ◽  
Esmeray Kuley Boğa ◽  
Ismail Akyol ◽  
Mustafa Durmus ◽  
Yılmaz Ucar ◽  
...  

LWT ◽  
2019 ◽  
Vol 113 ◽  
pp. 108246 ◽  
Author(s):  
Sujogya Kumar Panda ◽  
Yugal Kishore Mohanta ◽  
Laxmipriya Padhi ◽  
Walter Luyten

2013 ◽  
Vol 5 (3) ◽  
pp. 135-136 ◽  
Author(s):  
G. Sibi ◽  
Kalpana Kaushik ◽  
Kalegowda Dhananjaya ◽  
Kodagahally R. Ravikumar ◽  
Honnaiah Mallesha

1994 ◽  
Vol 8 (4) ◽  
pp. 13-18
Author(s):  
R. Brankova ◽  
B. Gyosheva ◽  
M. Michailova ◽  
G. Georgiev

2020 ◽  
pp. 1-6 ◽  
Author(s):  
Roberta Tardugno ◽  
Annalisa Serio ◽  
Chiara Purgatorio ◽  
Vincenzo Savini ◽  
Antonello Paparella ◽  
...  

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