scholarly journals Androgen receptor coregulator long noncoding RNA CTBP1-AS is associated with polycystic ovary syndrome in Kashmiri women

Endocrine ◽  
2021 ◽  
Author(s):  
Mudasar Nabi ◽  
Syed Mudasir Andrabi ◽  
Shayaq Ul Abeer Rasool ◽  
Sairish Ashraf ◽  
Imran Majid ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Polycystic Ovary Syndrome ◽  
Long Noncoding Rna ◽  
Noncoding Rna ◽  
Polycystic Ovary ◽  
Ovary Syndrome

scholarly journals Androgen Receptor Coregulator Long Non Coding RNA CTBP1-AS Is Associated With Polycystic Ovary Syndrome in Kashmiri Women

2021 ◽  
Author(s):  
Shajrul Amin ◽  
Mudasar Nabi ◽  
Syed Mudasir Andrabi ◽  
Shayaq Ul Abeer Rasool ◽  
Sairish Ashraf ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Polycystic Ovary Syndrome ◽  
Long Noncoding Rna ◽  
Noncoding Rna ◽  
Polycystic Ovary ◽  
Venous Blood ◽  
Premenopausal Women ◽  
Expression Level ◽  
Healthy Controls ◽  
Ovary Syndrome

Abstract Objective: Polycystic ovary syndrome (PCOS) is one of the most common reproductive, endocrine and metabolic disorders in premenopausal women. Even though the pathophysiology of PCOS is complex and obscure, the disorder is prominently considered as the syndrome of hyperandrogenism. C-Terminal binding protein 1 antisense (CTBP1-AS) acts as a novel Androgen Receptor regulating long noncoding RNA (lncRNA). Therefore, the present study was aimed to establish the possible association of androgen receptor regulating long noncoding RNA CTBP1-AS with PCOS.Methods: A total of 178 subjects including 105 PCOS cases and 73 age-matched healthy controls were recruited for the study. The anthropometric, hormonal and biochemical parameters of all subjects were analysed. Total RNA was isolated from peripheral venous blood and expression analysis was done by quantitative real time PCR (qRT-PCR). The correlation analysis was performed to evaluate the association between and various clinical parameters and lncRNA CTBP1-AS expression.Results and conclusion: the mean expression level of CTBP1-AS was found to be significantly higher in the PCOS women than in the healthy controls (-lnCTBP1-AS, 4.23 ± 1.68 versus 1.24 ± 0.29 p<0.001). Further, subjects with higher expression level of CTBP1-AS had significantly higher risk of PCOS compared to subjects with low levels of CTBP1-AS expression (actual OR = 11.36, 95% C.I. = 5.59-23.08, P = < 0.001). The area under ROC curve (AUC) was 0.987 (SE 0.006 and 95% C.I. 0.976–0.99). However, lncRNA CTBP1-AS was found to have no association with different clinical characteristics in PCOS. In conclusion, androgen receptor coregulating lncRNA CTBP1-AS is associated with PCOS women and high expression of CTBP1-AS is a risk factor for PCOS in Kashmiri women

scholarly journals OR08-2 Androgen Receptor- Vs Estrogen Receptor-Mediated Actions in the Development of Distinct Clinical Polycystic Ovary Syndrome (PCOS) Phenotypes

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Ali Aflatounian ◽  
Melissa Edwards ◽  
Robert Gilchrist ◽  
Michael Bertoldo ◽  
William Ledger ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Androgen Receptor ◽  
Polycystic Ovary Syndrome ◽  
Polycystic Ovary ◽  
Ovary Syndrome

Elevated Circulating Levels of Carnal ENST00000550337.1 Are Associated with Polycystic Ovary Syndrome in Chinese Women

2021 ◽  
pp. 1-7
Author(s):  
Yujuan Qi ◽  
Qianqian Yin ◽  
Juan Gu ◽  
Ying Liu ◽  
Qingqing Sun ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Peripheral Blood ◽  
Noncoding Rna ◽  
Chinese Women ◽  
Polycystic Ovary ◽  
Threshold Value ◽  
Expression Level ◽  
Ovary Syndrome ◽  
Blood Leukocytes ◽  
Cross Sectional

Objectives: Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disease. Some studies reported that the development of PCOS may be closely related to insulin resistance (IR). Interestingly, the long noncoding RNA (lncRNA) ENST00000550337.1 in peripheral blood is mainly involved in glucose metabolism. Therefore, the purpose of our study was to explore the relationship between lncRNA ENST00000550337.1 level and PCOS patients. Materials and Methods: Seventy-five PCOS patients and 72 healthy controls were enrolled in this study. We used qRT-PCR to detect the expression level of lncRNA ENST00000550337.1 in peripheral blood leukocytes from patients with PCOS. We also investigated potential relationships between lncRNA ENST00000550337.1 and the endocrine parameters in PCOS. Results: We observed that the expression of lncRNA ENST00000550337.1 in PCOS patients was significantly higher than that in the control subjects and positively correlated with PCOS occurrence, waist circumference, waist-hip ratio, IR, fasting insulin levels, and blood glucose. The expression of lnc RNA ENST00000550337.1 was positively correlated with PCOS (p = 0.003). There were independent correlations between IR and expression of lncRNA ENST00000550337.1 in patients with PCOS. Patients with elevated lncRNA ENST00000550337.1 expression had significantly increased PCOS risk after adjusting for age and BMI. LncRNA ENST00000550337.1 expression level provided a sensitivity of 81.3% and a specificity of 78.1% with a threshold value of 6.4648 for the prediction of PCOS. The area under the ROC was 0.813. Limitations: There are some limitations to this study. First, the sample size was limited and the causal relationship between lncRNA ENST00000550337.1 and PCOS was not investigated due to the cross-sectional study design. Second, HOMA-IR does not fully accurately reflect the IR of patients. Conclusions: The present study indicated that lnc RNA ENST00000550337.1 was related to PCOS occurrence, and elevated levels may be a risk factor for PCOS women. In addition, lncRNA ENST00000550337.1 might promote PCOS development partially by increasing IR and can be used as a potential molecular marker in patients with PCOS.

scholarly journals Stage-Specific Expression of Androgen Receptor, Follicle-Stimulating Hormone Receptor, and Anti-Müllerian Hormone Type II Receptor in Single, Isolated, Human Preantral Follicles: Relevance to Polycystic Ovaries

2007 ◽  
Vol 92 (3) ◽  
pp. 1034-1040 ◽  
Author(s):  
Suman Rice ◽  
Kamal Ojha ◽  
Saffron Whitehead ◽  
Helen Mason
Keyword(s):  
Androgen Receptor ◽  
Polycystic Ovary Syndrome ◽  
Polycystic Ovary ◽  
Negative Control ◽  
Type Ii ◽  
Polycystic Ovaries ◽  
Ovary Syndrome ◽  
Specific Expression ◽  
Primordial Follicles ◽  
Preantral Follicles

Abstract Context: Recent evidence indicates that the increase in follicle numbers seen in polycystic ovary syndrome occurs early in folliculogenesis, with androgens being a likely causative candidate. In primates and sheep, androgen excess in utero results in ovarian changes similar to those in polycystic ovary syndrome. There is also increasing interest in the role of anti-Müllerian hormone (AMH) in early folliculogenesis because AMH knockout mice have an early depletion of their stock of primordial follicles. Initiation and early folliculogenesis may therefore be under negative control by AMH and positive control by androgens. Objective: Because AMH signals exclusively through its type II receptor (AMHRII), the aim of this study was to determine and colocalize the stage-specific expression of AMHRII, androgen receptor (AR), and FSH receptor (FSHR) mRNA in individual, well-characterized preantral follicles. Method: Follicles were isolated from human ovarian cortex obtained from either oophorectomies or cortical biopsies at cesarean section. Expression of AR, FSHR, and AMHRII mRNA was determined using a nested RT-PCR protocol. Results: AR mRNA was not detected in any primordial follicles but was from the transitional stage onward. The number of AR-positive follicles increased at each progressive growth stage. The expression of AR preceded that of FSHR, and only a small percentage of primary follicles expressed FSHR. AMHRII expression was rarely detected. Conclusions: This is the first study to identify the expression of AR in human transitional follicles. Results suggest a role for androgens in promoting early follicle growth and challenging the hypothesis that AMH exerts a direct, inhibitory effect on follicles at this stage.

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