scholarly journals Preliminary results of indole alkaloids production in different roots of Catharanthus roseus cultured in vitro

2014 ◽  
Vol 70 (4) ◽  
pp. 261-265 ◽  
Author(s):  
Agnieszka Pietrosiuk ◽  
Mirosława Furmanowa
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Roots ◽  
Catharanthus Roseus ◽  
Indole Alkaloids ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Preliminary Results ◽  
Intact Plants

Six groups of untransformed and hairy root cultures of <em>Catharunthus roseus</em> (L.) G. Don were established. <em>Agrobacterium rhizogenes</em> strains: ATCC 15834, LBA 9403, and TR 105 were used for infection of the 3-week old rooted plantlets of <em>C. roseus</em>. The highest contents of examined indole alkaloids were found in: roots of intact plants - yohimbine and serpentine; in hairy roots - catharanthine. Vinblastine and ajmalicine were detected in untransformed roots of plants regenerated in vitro, and transferred to the soil for 5 months.

scholarly journals Alterations in oleanolic acid and sterol content in marigold (Calendula officinalis) hairy root cultures in response to stimulation by selected phytohormones

2021 ◽  
Vol 43 (3) ◽  
Author(s):  
Abdulwadood S. M. Alsoufi ◽  
Klaudia Staśkiewicz ◽  
Michał Markowski
Keyword(s):  
Oleanolic Acid ◽  
Hairy Root ◽  
Hairy Roots ◽  
Naphthaleneacetic Acid ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Calendula Officinalis ◽  
Plant Metabolites ◽  
Triterpenoid Saponins

AbstractHairy root cultures are an efficient tool for the biotechnological production of plant metabolites and a convenient experimental model for analyzing the effect of various compounds on plant metabolism. In contrast to many other types of in vitro plant cultures, hairy roots do not require an external supply of phytohormones to the medium. Consequently, plant growth regulators such as auxins and cytokinins are rarely used as elicitors in hairy root in vitro cultures; however, they can strongly influence plant defense responses. The aim of this study was to investigate the influence of two auxins: natural indole-3-acetic acid (IAA) and synthetic 1-naphthaleneacetic acid (NAA), as well as two cytokinins: natural kinetin and synthetic 6-benzylaminopurine (BAP) at a concentration of 0.75 mg/L on the metabolism of sterols and triterpenoids in Calendula officinalis hairy roots. Auxins prevented the accumulation of triterpenoid saponins (oleanolic acid glycosides), while cytokinin BAP increased their accumulation by 17% and their release into the culture medium by a factor of 10. Other cytokinins and kinetins increased the sterol levels by 17%, the level of stigmasterol by 15%, and the level of isofucosterol by 7 times.

scholarly journals Role of Different Transporting Systems in the Secretion of Alkaloids by Hairy Roots of Catharanthus roseus (L) G. Don

2017 ◽  
Vol 59 (2) ◽  
Author(s):  
Víctor Manuel Loyola-Vargas
Keyword(s):  
Acetylsalicylic Acid ◽  
Hairy Roots ◽  
Catharanthus Roseus ◽  
Abc Transporters ◽  
Indole Alkaloids ◽  
Hairy Root Cultures ◽  
Biosynthetic Pathways ◽  
Root Cultures ◽  
Transport Inhibitors

<p>Knowledge on the biosynthetic pathways of the monoterpene alkaloids is enormous, but little is known about their mechanism of transporting system from the plant cell. There is not concrete evidence confirming the role of ABC transporters in the secretion of monoterpene indole alkaloids (MIAs) in <em>Catharanthus roseus</em>. Therefore, in order to determine the role of different transporting systems involved in the MIAs translocation, we employed a pharmacological approach by using transport inhibitors such as, KCN, Na3VO4, quinidine and glibenclamide in hairy root cultures of <em>C. roseus</em>. It was found that the accumulation of ATP drastically decreased in the presence of KCN or 100 μM acetylsalicylic acid (ASA)/100 μM KCN. The treatment with the inhibitors KCN and glibenclamide in the presence of ASA significantly increased the ajmalicine secretion compared to the control. The secretion of serpentine was undetected during the first 24 h in all the samples. Treatment with the inhibitors quinidine and glibenclamide provoked a significant reduction of serpentine secretion in the hairy roots compared to the control. Based on our results, we found evidence that ABC transporters might participate in the secretion of MIAs by <em>C. roseus </em>hairy roots.</p>

scholarly journals Production of Triterpenoid Sapogenins in Hairy Root Cultures of Silene vulgaris

2015 ◽  
Vol 10 (11) ◽  
pp. 1934578X1501001
Author(s):  
Yeon Bok Kim ◽  
Darwin W. Reed ◽  
Patrick S. Covello
Keyword(s):  
Hairy Root ◽  
Hairy Roots ◽  
Leaf Explants ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Silene Vulgaris ◽  
In Vitro Production ◽  
Triterpenoid Saponins ◽  
Meja Treatment

Silene vulgaris (Moench) Garcke (Caryophyllaceae) is widely distributed in North America and contains bioactive oleanane-type saponins. In order to investigate in vitro production of triterpenoid saponins, hairy root cultures of S. vulgaris were established by infecting leaf explants with five strains of Agrobacterium rhizogenes (LBA9402, R1000, A4, 13333, and 15834). The A. rhizogenes strain LBA9402 had an infection of 100% frequency and induced the most hairy roots per plant. Methyl jasmonate (MeJA)-induced changes in triterpenoid saponins in S. vulgaris hairy roots were analyzed. Accumulation of segetalic acid and gypsogenic acid after MeJA treatment was 5-and 2-fold higher, respectively, than that of control root. We suggest that hairy root cultures of S. vulgaris could be an important alternative approach to the production of saponins.

Sustainable bioproduction of phytochemicals by plant in vitro cultures: anticancer agents

2005 ◽  
Vol 3 (2) ◽  
pp. 90-100 ◽  
Author(s):  
Michael Wink ◽  
A. Wilhelm Alfermann ◽  
Rochus Franke ◽  
Bernhard Wetterauer ◽  
Melanie Distl ◽  
...  
Keyword(s):  
Hairy Root ◽  
Hairy Roots ◽  
Anticancer Agents ◽  
Complex Structure ◽  
Suspension Cultures ◽  
Cell Suspensions ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Commercial Scale

Due to their complex structure with several chiral centres important anticancer agents are still extracted from plants and not synthesized chemically on a commercial scale. Sustainable bioproduction of the compounds of interest may be achieved by plant in vitro cultures. Undifferentiated callus and suspension cultures, which can be cultivated in large bioreactors easily, very often fail to accumulate the compounds of interest, whereas shoot and root cultures as well hairy roots normally produce the same compounds as in the appropriate organs. The production of anticancer compounds, such as the alkaloids vinblastine, vincristine, paclitaxel (Taxol®), camptothecin, or the lignan podophyllotoxin, by plant in vitro cultures is reviewed. Taxanes can be produced in bioreactors using cell suspensions of various Taxus species with good yields; presently paclitaxel is produced on a commercial scale by Phyton Biotech (Germany). Camptothecin has low yields in suspension cultures of Camptotheca acuminata or Nothapodytes foetida (0.0003–0.01%), but a good production (0.1–0.3% dry wt) in root and hairy root cultures of Ophiorrhiza pumila, O. mungos and C. acuminata. Podophyllotoxin can be produced in cell suspension and root as well as hairy root cultures of Podophyllum and various Linum species up to 130 mg/l (Linum album cell suspensions); its derivative 6-methoxypodophyllotoxin is accumulated in hairy roots of L. persicum up to about 500 mg/l. The in vitro production of dimeric indole alkaloids in Catharanthus roseus has failed so far both in undifferentiated and differentiated in vitro cultures. In cases where in vitro cultures show good yields, they can be employed in biotechnology for the sustainable production of valuable products.

Plant regeneration from hairy-root cultures transformed by infection with Agrobacterium rhizogenes in Catharanthus roseus

2004 ◽  
Vol 22 (11) ◽  
pp. 828-831 ◽  
Author(s):  
P.S. Choi ◽  
Y.D. Kim ◽  
K.M. Choi ◽  
H.J. Chung ◽  
D.W. Choi ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Catharanthus Roseus ◽  
Hairy Root Cultures ◽  
Root Cultures
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