scholarly journals Effects of multi-wall carbon nanotubes on structural and mechanical properties of poly(3-hydroxybutyrate)/chitosan electrospun scaffolds for cartilage tissue engineering

2017 ◽  
Vol 40 (6) ◽  
pp. 1247-1253 ◽  
Author(s):  
Saeed Karbasi ◽  
Zahra Mohammad Alizadeh
Keyword(s):  
Mechanical Properties ◽  
Carbon Nanotubes ◽  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Multi Wall Carbon Nanotubes ◽  
Electrospun Scaffolds

scholarly journals Preparation and Characterization of Nanofibrous Polymer Scaffolds for Cartilage Tissue Engineering

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Jarosław Markowski ◽  
Anna Magiera ◽  
Marta Lesiak ◽  
Aleksander L. Sieron ◽  
Jan Pilch ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Carbon Nanotubes ◽  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Physical And Mechanical Properties ◽  
Biological Assessment ◽  
Cartilage Tissue ◽  
Poly Lactic Acid ◽  
Fibrous Membranes

Polymer substrates obtained from poly(lactic acid) (PLA) nanofibres modified with carbon nanotubes (CNTs) and gelatin (GEL) for cartilage tissue engineering are studied. The work presents the results of physical, mechanical, and biological assessment. The hybrid structure of PLA and gelatine nanofibres, carbon nanotubes- (CNTs-) modified PLA nanofibres, and pure PLA-based nanofibres was manufactured in the form of fibrous membranes. The fibrous samples with different microstructures were obtained by electrospinning method. Microstructure, physical and mechanical properties of samples made from pure PLA nanofibres, CNTs-, and gelatin-modified PLA-nanofibres were studied. The scaffolds were also testedin vitroin cell culture of human chondrocytes collected from patients. To assess the influence of the nanofibrous scaffolds upon chondrocytes, tests for cytotoxicity and genotoxicity were performed. The work reveals that the nanofibrous structures studied were neither genotoxic nor cytotoxic, and their microstructure, physical and mechanical properties create promising scaffolds for potential use in cartilage repairing.

Application of Carbon Nanotubes in Cartilage Tissue Engineering

2008 ◽  
Author(s):  
Nadeen O. Chahine ◽  
Nicole M. Collette ◽  
Heather Thompson ◽  
Gabriela G. Loots
Keyword(s):  
Carbon Nanotubes ◽  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cellular Systems ◽  
Cellular Growth ◽  
Cartilage Tissue ◽  
Chondrocyte Viability ◽  
Structural Reinforcement ◽  
Surgical Implants ◽  
Novel Material

Carbon nanotubes (CNTs) are cylindrical allotropes of carbon that are nanometers in diameter and posses unique physical properties, positioning them as ideal materials for studying physiology at a single cell level. CNTs have the potential to become a very important component of medical therapeutics, likely acting as (a) drug delivery system [1], (b) existing as an interfacial layer in surgical implants [2,3], or (c) acting as scaffolding in tissue engineering [4,8]. While some studies have explored the use of CNTs as a novel material in regenerative medicine, they have not yet been fully evaluated in cellular systems. One major limitation of CNTs that must be overcome is their inherent cytotoxicity. The goal of this study is to assess the long-term biocompatibility of CNTs for chondrocyte growth. We hypothesize that CNT-based material in tissue engineering can provide an improved molecular sized substrate for stimulation of cellular growth, and structural reinforcement of the scaffold mechanical properties. Here we present data on the effects of CNTs on chondrocyte viability and biochemical deposition examined in composite materials of hydrogels + CNTs mixtures. Also, the effects of CNTs surface functionalization with polyethlyne glycol (PEG) or carboxyl groups (COOH) were examined.

Polyethylene glycol diacrylate scaffold filled with cell-laden methacrylamide gelatin/alginate hydrogels used for cartilage repair

2021 ◽  
pp. 088532822110448
Author(s):  
Xiang Zhang ◽  
Zhenhao Yan ◽  
Guotao Guan ◽  
Zijing Lu ◽  
Shujie Yan ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Polyethylene Glycol ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
High Concentration ◽  
Polyethylene Glycol Diacrylate ◽  
Low Concentration ◽  
Glycol Diacrylate

Natural cartilage tissue has excellent mechanical properties and has certain cellular components. At this stage, it is a great challenge to produce cartilage scaffolds with excellent mechanical properties, biocompatibility, and biodegradability. Hydrogels are commonly used in tissue engineering because of their excellent biocompatibility; however, the mechanical properties of commonly used hydrogels are difficult to meet the requirements of making cartilage scaffolds. The mechanical properties of high concentration polyethylene glycol diacrylate (PEGDA) hydrogel are similar to those of natural cartilage, but its biocompatibility is poor. Low concentration hydrogel has better biocompatibility, but its mechanical properties are poor. In this study, two different hydrogels were combined to produce cartilage scaffolds with good mechanical properties and strong biocompatibility. First, the PEGDA grid scaffold was printed with light curing 3D printing technology, and then the low concentration GelMA/Alginate hydrogel with chondral cells was filled into the PEGDA grid scaffold. After a series of cell experiments, the filling hydrogel with the best biocompatibility was screened out, and finally the filled hydrogel with cells and excellent biocompatibility was obtained. Cartilage tissue engineering scaffolds with certain mechanical properties were found to have a tendency of cartilage formation in in vitro culture. Compared with the scaffold obtained by using a single hydrogel, this molding method can produce a tissue engineering scaffold with excellent mechanical properties on the premise of ensuring biocompatibility, which has a certain potential application value in the field of cartilage tissue engineering.

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