Cyclooxygenase (COX) Inhibition by Acetyl Salicylic Acid (ASA) Enhances Antitumor Effects of Nitric Oxide in Glioblastoma In Vitro

The methanol extract of the whole plant of Blumea lacera (Burn.f.) DC. (BLME) has been subjected to preliminary screenings for phytoconstituents and antipyretic, analgesic and anti-inflammatory activities. Antipyretic activity was assessed by the yeast-induced hyperthermia in mice. The analgesic property was evaluated by formalin-induced writhing test. Acetyl salicylic acid (ASA) was used as standard for in-vitro anti-inflammatory activity test. In yeast-induced pyrexia, the crude extract demonstrated a significant (p=0.05) reduction in body temperature of mice after elevation by the administration of yeast. These effects were pronounced at the 2nd and 3rd h of post-treatment with the extract. BLME exhibited a dose-dependent analgesic activity with 39.13% and 56.52% protection at 200-and 400-mg/kg, b.w., respectively as compared to 76.09% revealed by the standard diclofenac sodium. In the anti-inflammatory test, the crude extract at 400 ?g/ml displayed 62.40% inhibition of protein denaturation whereas standard acetyl salicylic acid exhibited 76.74% inhibition. Results of the preliminary phytochemical screenings demonstrated the presence of alkaloids, flavonoids and triterpenoids in the extract. DOI: http://dx.doi.org/10.3329/dujps.v13i1.21863 Dhaka Univ. J. Pharm. Sci. 13(1): 69-73, 2014 (June)

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The effect of acetyl salicylic acid (Aspirin) on trophoblast-endothelial interaction in vitro

Journal of Reproductive Immunology ◽

10.1016/j.jri.2017.10.044 ◽

2017 ◽

Vol 124 ◽

pp. 54-61 ◽

Cited By ~ 14

Author(s):

Bei Xu ◽

Renuka Shanmugalingam ◽

Katrina Chau ◽

Suzanne Pears ◽

Annemarie Hennessy ◽

...

Keyword(s):

Salicylic Acid ◽

Acetyl Salicylic Acid

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Acetylsalicylic acid and salicylic acid present anticancer properties against melanoma by promoting nitric oxide-dependent endoplasmic reticulum stress and apoptosis

Scientific Reports ◽

10.1038/s41598-020-76824-6 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Priscila Ausina ◽

Jessica R. Branco ◽

Thainá M. Demaria ◽

Amanda M. Esteves ◽

João Gabriel B. Leandro ◽

...

Keyword(s):

Nitric Oxide ◽

Endoplasmic Reticulum ◽

Salicylic Acid ◽

Acetylsalicylic Acid ◽

Er Stress ◽

In Vitro Models ◽

Anticancer Effects ◽

Factor C

AbstractMelanoma is the most aggressive and fatal type of skin cancer due to being highly proliferative. Acetylsalicylic acid (ASA; Aspirin) and salicylic acid (SA) are ancient drugs with multiple applications in medicine. Here, we showed that ASA and SA present anticancer effects against a murine model of implanted melanoma. These effects were also validated in 3D- and 2D-cultured melanoma B16F10 cells, where the drugs promoted pro-apoptotic effects. In both in vivo and in vitro models, SA and ASA triggered endoplasmic reticulum (ER) stress, which culminates with the upregulation of the pro-apoptotic transcription factor C/EBP homologous protein (CHOP). These effects are initiated by ASA/SA-triggered Akt/mTOR/AMPK-dependent activation of nitric oxide synthase 3 (eNOS), which increases nitric oxide and reactive oxygen species production inducing ER stress response. In the end, we propose that ASA and SA instigate anticancer effects by a novel mechanism, the activation of ER stress.

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