scholarly journals Preliminary phytochemical screenings and Pharmacological activities of Blumea lacera (Burn.f.) DC.

2015 ◽  
Vol 13 (1) ◽  
pp. 69-73
Author(s):  
Md Abul Khair ◽  
Mohammed Ibrahim ◽  
Qamrul Ahsan ◽  
Md Ruhul Kuddus ◽  
Ridwan Bin Rashid ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Crude Extract ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Acetyl Salicylic Acid ◽  
Antipyretic Activity ◽  
Whole Plant ◽  
Anti Inflammatory ◽  
Blumea Lacera

The methanol extract of the whole plant of Blumea lacera (Burn.f.) DC. (BLME) has been subjected to preliminary screenings for phytoconstituents and antipyretic, analgesic and anti-inflammatory activities. Antipyretic activity was assessed by the yeast-induced hyperthermia in mice. The analgesic property was evaluated by formalin-induced writhing test. Acetyl salicylic acid (ASA) was used as standard for in-vitro anti-inflammatory activity test. In yeast-induced pyrexia, the crude extract demonstrated a significant (p=0.05) reduction in body temperature of mice after elevation by the administration of yeast. These effects were pronounced at the 2nd and 3rd h of post-treatment with the extract. BLME exhibited a dose-dependent analgesic activity with 39.13% and 56.52% protection at 200-and 400-mg/kg, b.w., respectively as compared to 76.09% revealed by the standard diclofenac sodium. In the anti-inflammatory test, the crude extract at 400 ?g/ml displayed 62.40% inhibition of protein denaturation whereas standard acetyl salicylic acid exhibited 76.74% inhibition. Results of the preliminary phytochemical screenings demonstrated the presence of alkaloids, flavonoids and triterpenoids in the extract. DOI: http://dx.doi.org/10.3329/dujps.v13i1.21863 Dhaka Univ. J. Pharm. Sci. 13(1): 69-73, 2014 (June)

scholarly journals Insights into the Invitro Antioxidant, Anti-Inflammatory and Anticancer Activities of Limonia acidissima Fruits

2021 ◽  
Vol 11 (3) ◽  
Author(s):  
S. Sujitha
Keyword(s):  
Ascorbic Acid ◽  
Aqueous Extract ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Apple Fruit ◽  
Fruit Pulp ◽  
Anticancer Activities ◽  
Anti Inflammatory ◽  
Limonia Acidissima

Wood apple botanically identified as Limonia acidissima is an indigenous fruit with amazing nutritional and health benefits. Reports from traditional literature of Ayurveda and Siddha portrays the medicinal properties of this fruit. The scooped pulp of the ripe fruit is consumed as such or it can be made into different recipes. But inclusion of this fruit in our diet is not found as a regular practice. Many people are still unaware of the benefits of this fruit. Hence, this study was taken up to unravel the biological potencies of this fruit by conducting in vitro experiments. Phytochemicals such as alkaloids, flavonoids, phenols, saponins and ascorbic acid have been estimated. Anti-inflammatory activity of the aqueous extract of fruit pulp combined with outer rind has been evaluated through inhibition of albumin denaturation. Among the 5 different concentrations (200, 400, 600, 800, 1000 µg/ml), at 1000 µg/ml wood apple has shown 74.55% of protein denaturation inhibition which was compared with standard Diclofenac sodium. Antioxidant capacity of the extract was expressed as mg/100g ascorbic acid equivalent through phosphomolybdenum assay. Dose dependent increase in the antioxidant activity was observed. About 8 different concentrations of the aqueous extract of L. acidissima were evaluated for their cytotoxic activity on MCF 7 cell line. At a concentration of 1000 µg/ml, the extract has shown 93.43% of cytotoxicity and 6.57% of cell viability. Apoptotic induction was evaluated and confirmed by the formation of DNA ladders through DNA fragmentation assay. GCMS analysis of wood apple fruit pulp and rind revealed the presence of several phytochemicals among which many of them had therapeutic activity reported earlier.

scholarly journals Phytochemical Screening and In Vitro Antioxidant and Anti-inflammatory Activities of Aerial Parts of Euphorbia hirta L.

2021 ◽  
Vol 42 (1) ◽  
pp. 115-124
Author(s):  
Deepak Basyal ◽  
Astha Neupane ◽  
Durga Prasad Pandey ◽  
Shiva Pandeya
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Euphorbia Hirta ◽  
Anti Inflammatory

Euphorbia hirta L (Euphorbiaceae) also called asthma herb has long been prescribed in traditional medicine because it exhibits diverse pharmacological actions due to the presence of alkaloids, flavonoids, polyphenols, triterpenoids, and saponins. The present study is aimed at the study of phytochemical and antioxidant activity and anti-inflammatory screening of E. hirta. Extraction of dried powder was performed followed by phytochemical screening using color reactions. Total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were estimated by Folin-Ciocalteu and Aluminum chloride method respectively. The antioxidant activity was studied by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method.  Anti-inflammatory activity was studied by using protein denaturation in vitro bioassay. Phytochemical screening showed the presence of flavonoids, alkaloids, and phenolic compounds. TPC, TFC and antioxidant activity (IC50) of the extract were found as 288.10 mg gallic acid equivalent per gram (GAE/g), 29.36 mg quercetin equivalent per gram (QE/g) and 32.23 µg/mL (p<0.05) respectively. Diclofenac sodium and E. hirta extract showed the maximum inhibition of 91.28% and 68.20% respectively at the concentration of 1000 µg/mL compared with control (p>0.05). The phenolic compounds and flavonoids exert antioxidant and anti-inflammatory activities because of their scavenging ability. The demonstrated antioxidant and anti-inflammatory activities may be the rationale behind some of its folkloric uses and also may be responsible for some of its pharmacological effects. Thus, E. hirta can be considered a good source of antioxidants and anti-inflammatory actions, which might be beneficial for combating oxidative stress.

scholarly journals In-Vitro Anti-inflammatory Activity of Methanolic Extract of Convolvulus pluricaulis Choisy

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Puneet Singh ◽  
Yogesh Sharma ◽  
Ashutosh Sharma
Keyword(s):  
Methanolic Extract ◽  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Local Market ◽  
Plant Materials ◽  
Whole Plant ◽  
Soxhlet Apparatus ◽  
Anti Inflammatory ◽  
Convolvulus Pluricaulis

Aim: In-Vitro Anti-inflammatory Activity of Methanolic Extract of Convolvulus pluricaulis Choisy. Material & Methods- The whole plant parts of Convolvulus pluricaulis Choisy were purchased from the local market. Whole plant materials were dried under shade and subjected to coarse powder for extraction process. Accurately weighed quantity of whole plant material was extracted using 95 % methanol by soxhlet apparatus for 72 h. Qualitative chemical tests of methanolic extracts were subjected to various chemical tests to detect various phytoconstituents. Solvent systems ethyl acetate: methanol: water (77:13:10) were found to be most satisfactory solvent system. After development of plates, they were air-dried and number of spots, color and Rf values were recorded. The % heamolysis was calculated by assuming the heamolysis produced by the control group as 100 %. Results: The preliminary phytochemical analysis revealed that different active constituent present in different extracts such as carbohydrates, proteins, amino acids, fat, oils, steroids, terpenoids, glycosides, alkaloids, tannins and other phenolics compounds. At a concentration of 500 µg/ml, the extract produced 71.59% protection of RBC haemolysis as compared with 72.73% produced by prednisolone.  The methanolic extract of selected plant showed 39.70% inhibition. The Diclofenac sodium showed 55.88 % inhibition against denaturation of protein. Conclusion: In conclusion, it can be stated that the methanolic extract has beneficial effects in long lasting in membrane stabilizing method, inhibition of protein denaturation method and proteinase model. Keywords: In-Vitro, Anti-inflammatory Activity, Methanolic Extract, Convolvulus pluricaulis Choisy, Protein Denaturation Method

IN VITRO ANTIARTHRITIC AND ANTI-INFLAMMATORY ACTIVITY OF PETETHER EXTRACT OF HYPTIS SUAVEOLENS

INDIAN DRUGS ◽  
2016 ◽  
Vol 53 (09) ◽  
pp. 60-62
Author(s):  
M. R Elayaraja Krishnan ◽  
◽  
R. Vijaya Kanth ◽  
S. Muthu Pandi
Keyword(s):  
Diclofenac Sodium ◽  
Inflammatory Activity ◽  
Ether Extract ◽  
In Vitro Methods ◽  
Whole Plant ◽  
Hyptis Suaveolens ◽  
Excellent Activity ◽  
Anti Inflammatory ◽  
The Stability

In our present study, various concentrations of pet ether extract of whole plant of Hyptis suaveolens were prepared and subjected for anti arthritic and anti-inflammatory activity by in vitro methods. The antiarthritic activity was carried out by bovine serum albumin denaturation paradigm. The results showed that the pet ether extract (44.51%) showed excellent arthritic activity when compared to the standard diclofenac sodium (31.44%) at 2000μg/mL. Meanwhile, in the anti-inflammatory paradigm, the same extract (99.10%) showed excellent activity than the standard diclofenac sodium (73.80%) at 2000μg/ mL by restoring the stability of degraded red blood corpuscles. In both the paradigms, increase in concentration of the extract increases the action potential against the methanol degradation of bovine albumin and HRBC.

Pharmacological and Biochemical evaluation of anti-arthritic activity of Justicia gendarussa extract in FCA induced arthritis in Wistar rats

2021 ◽  
Vol 12 (3) ◽  
pp. 1699-1708
Author(s):  
Ashish ◽  
Anjali ◽  
Praveen K Dixit ◽  
Nagarajan K
Keyword(s):  
Body Weight ◽  
Protein Denaturation ◽  
Hematological Parameters ◽  
Diclofenac Sodium ◽  
Paw Edema ◽  
Arthritis Model ◽  
Inflammatory Models ◽  
Anti Inflammatory ◽  
Inflammatory Effect

The present study is carried out to evaluate anti-inflammatory and anti-arthritic potential of Justicia gendarussa. Leaves and stem extracted with ethanol and chloroform. These extract is tested against in-vitro (HRBC membrane stabilization method and Inhibition of protein denaturation method) and in-vivo (Carrageenan induced paw edema model) anti-inflammatory models. To assess the anti-arthritic activity, FCA induced arthritis model is used. Assessment of arthritis is done by paw volume, joint diameter, body weight, biochemical parameters, hematological parameters (Hb, RBC, WBC, ESR). The effect of in-vitro anti-inflammatory model depends on concentration. Both test extract and standard Diclofenac sodium has been shown concentration dependent effect. The maximum anti-inflammatory effect of the test extract achieved at 2000μg/ml. The test (JGLE, JGLC, JGSE) has been shown inhibition of paw edema induced by carrageenan at 50mg/kg body weight. The extract JGLE, JGLC, JGSE at 50mg/kg body weight and 100mg/kg body weight orally showed the significant (P< 0.05) and dose dependent inhibitory effect against FCA induced arthritis model. Diclofenac sodium 20mg/kg body weight orally is used as a standard. JGLE exhibit more significant and most promising anti-arthritic and anti-inflammatory effect than other extracts these effects support the traditional role of J. gendarussa in arthritis and other inflammatory condition.

scholarly journals PHYTOCHEMICAL AND IN-VITRO PHARMACOLOGICAL SCREENING OF OCIMUM KILIMANDSCHARICUM EXTRACT

2020 ◽  
pp. 251-257
Author(s):  
Lahari.Sidde Lahari.Sidde ◽  
Motte.Sushma Motte.Sushma ◽  
Bandi.Jayanthi Bandi.Jayanthi
Keyword(s):  
Tamil Nadu ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Developed Countries ◽  
Small Scale ◽  
Standard Drug ◽  
Ethanolic Extraction ◽  
Anti Inflammatory ◽  
Pharmacological Screening

Ocimum kilimandscharicum is a short herb, native to India. In traditional medicine, it is used to treat various ailments including colds, coughs, abdominal pains, measles and diarrhea. Medicative plants, the “back bone” of ancient medication which implies over 3.3 billion folks within the less developed countries utilize medicative plants on an everyday basis. Ocimum kilimandscharicum Guerke (Syn. Ocimum camphora Guerke) belongs to family Lamiaceae. It is a native of Kenya and distributed in East Africa, India, Thailand, Uganda and Tanzania. It is extensively grown in the Tropics. In India it is cultivated on a small scale, especially in West Bengal, Assam, Tamil Nadu, Karnataka, Kerala and Dehradun. .The present study is aimed to evaluate in vitro anti-inflammatory, assessment was carried out on the basis of parameters such as heat induced protein denaturation, heat induced haemolysis, and Hypotonicity induced haemolysis. The standard drug was Diclofenac sodium. The findings of  the present study showed that the 500 µg/ml of Ethanolic extraction of Ocimum kilimandscharicum ( EEOK) showed more percentage inhibition when compared to standard. On the basis of results, it can be concluded that Ethanolic extraction of Ocimum kilimandscharicum (EEOK) showed significant anti- inflammatory activity.

scholarly journals In-vitro Antioxidant and Anti-inflammatory Activities of Quisqualis indica Linn. Leaves Extract

2019 ◽  
pp. 1-13
Author(s):  
Pallavi Pal ◽  
Ajeet Singh
Keyword(s):  
Protein Denaturation ◽  
Radical Scavenging Activity ◽  
Diclofenac Sodium ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Standard Drug ◽  
Anti Inflammatory

Aim: In this study antioxidant and anti-inflammatory effect of ethanolic extract of Quisqualis indica leaves was evaluated. Study Design: In-vitro analysis of Quisqualis indica leaf extract. Place and Duration of Study: Molecular Biology laboratory, Department of Biotechnology, G.B Pant Engineering College, Pauri, between July 2015 and July 2016. Methods: Non-enzymatic and enzymatic assays such as DPPH (1, 1diphenyl-2-picryl hydrazyl), FRAP assay, superoxide dismutase SOD (EC 1.15.1.1), catalase (EC 1.11.1.6), for radical scavenging activity of ethanolic extracts of Quisqualis indica Linn. plant leaves had done. For estimation of anti-inflammatory action, two methods were employed: protein denaturation method and membrane stabilization method. Results: Ethanolic extract of leaves on higher concentration had better antioxidant potential when compared with reference standard ascorbic acid. They exhibited strong antioxidant radical scavenging activity values for ethanolic extract of leaves. Results of anti-inflammatory method suggested better potential values for ethanolic extract and compared with standard drug diclofenac sodium respectively. A significant relationship between antioxidant, anti-inflammatory capacity and total phenolic content was examined, indicating that phenolic compounds are the major contributors for the antioxidant and anti-inflammatory properties of this plant. Conclusion: Ethanolic extract of Q. indica exhibited strong anti-inflammatory and antioxidant activity and this can be used for designing novel drug inhibitors with better efficacy.

IN VITRO ANTI-INFLAMMATORY ACTIVITY OF METHANOLIC EXTRACT AND DIFFERENT FRACTIONS OF CENTELLA ASIATICA LEAVES BY PROTEIN DENATURATION

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (06) ◽  
pp. 86-89
Author(s):  
S Sharma ◽  
◽  
R. Trivedi ◽  
N. K. Choudhary
Keyword(s):  
Petroleum Ether ◽  
Skin Diseases ◽  
Methanolic Extract ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Centella Asiatica ◽  
Inflammatory Activity ◽  
Standard Drug ◽  
Anti Inflammatory

Inflammation might be a complex organic reaction to a hazardous stimulant such as pathogens, or injured tissues and mainly causes itching, swelling, skin redness, warm and slight pain. Herbal drugs are widespread in India for their effectiveness, easy availability at low cost and provide low toxicity as compared to modern drugs. Centella asiatica is one of the oldest Ayurvedic medicinal plants, used in treatment of various skin diseases. The aim of our present research was to evaluate the in vitro anti-inflammatory activity of methanolic extract and different fractions of C. asiatica leaves. In protein denaturation method, the percentage inhibition for methanolic extract was observed to be 40.22%. The petroleum ether and n-butanol fraction of methanolic extract of C. asiatica were observed to exhibits 54.12 and 44.42% inhibition, respectively. Diclofenac sodium was used as a standard drug. In comparison with other fractions petroleum ether and n-butanol fractions showed best activity. The preliminary phytochemical studies of n-butanol fractions and n-butanol fractions showed the presence of terpenoids, flavonoids etc., which are used in the treatment of inflammation. Thus, we can call the latter as intense anti-inflammatory agent.

scholarly journals In vitro and In vivo Anti-inflammatory Activity of the Extracts of Whole Plant Argyreia imbricata (Roth) Sant. & Patel

2020 ◽  
Vol 11 (4) ◽  
pp. 7317-7322
Author(s):  
Sebastin V ◽  
Gopalakrishnan G ◽  
Sreejith M ◽  
Anoob Kumar K I
Keyword(s):  
Ethyl Acetate ◽  
Diclofenac Sodium ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Significant Activity ◽  
In Vivo Evaluation ◽  
Whole Plant ◽  
Anti Inflammatory

Plants of the genus Argyreia have ethnomedicinal importance, and several pharmacological activities are also reported. In this study, the anti-inflammatory activity of different extracts of Argyreia imbricata was evaluated by in vitro and in vivo methods. In both evaluations, standard, Diclofenac sodium was used for comparative evaluation. In this study, extraction of powdered whole plant material was done with different solvents viz., petroleum ether, chloroform, ethyl acetate and methanol by soxhelation. In vitro anti-inflammatory activity of all the prepared extracts was evaluated by stabilization of human red blood cell (HRBC) membrane in different temperature and tonicity conditions. Among the six different concentrations of four tested extracts, the ethyl acetate and methanol extracts (1000μg/ml) showed significant activity in the in vitro evaluation. They were selected for the in vivo evaluation on the paw oedema induced by carrageenan on Wistar albino rats. Two doses, 200mg.kg-1 and 400mg.kg-1 of the test extracts were subjected to evaluation. Both the tested extracts showed the activity, particularly, the methanol extract in the dose of 400mg.kg-1 showed significant activity. Results of this study strongly supported the anti-inflammatory activity of the tested extracts. Further, studies on toxicity, identification, isolation of the active constituents may give useful results.

scholarly journals Assessment of In vitro Anti-inflammatory Activity of Ginger and Diclofenac sodium combination

2020 ◽  
pp. 442-447
Author(s):  
Mousmi D. Thakur ◽  
Navin R. Sheth ◽  
Mihir K. Raval
Keyword(s):  
Methanolic Extract ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Research Work ◽  
Pharmacological Action ◽  
Inflammatory Activity ◽  
Membrane Stabilization ◽  
Ginger Extract ◽  
Anti Inflammatory

The present research work aimed at evaluating the anti-inflammatory activity of Zingiber officinalis with Diclofenac sodium by HRBC membrane stabilization & Protein denaturation. The precluding of hypotonicity induced HRBC membrane lysis was taken as a measure of the anti-inflammatory activity. The percentage of membrane stabilization at different concentrations was performed for methanolic, hydro-methanolic ginger extract and diclofenac sodium. At a dose of 50µg/ml the maximum membrane stabilization 86.34% was found for Ginger extract(test) and at a dose of 500 mcg/ml membrane stabilization was found 91.16% for diclofenac sodium(standard) and the membrane stabilization for combination (ginger with diclofenac sodium) at a dose of 50µg/ml was recorded 86.43%, as the concentration increase(1000 mcg/ml) for combination(ginger with diclofenac sodium) the percentage protection was decreased. In vitro protein denaturation was performed by using egg albumin method. Maximum inhibition was observed in case of methanolic extract of ginger at concentration 1000mcg/ml and it was 78.83±5.17 and in hydro methanolic extract for Diclofenac sodium at concentration 1000mcg/ml and it was 63.37±2.78.Minimum inhibition observed in combination of methanolic extract of ginger and diclofenac sodium at concentration 1000mcg/ml and it was 25.27±1.76 and in combination of hydro-methanolic extract of ginger and Diclofenac sodium at concentration 1000mcg/ml and it was 28.23±3.14. The results of this study divulge that low dose combination of ginger and diclofenac sodium has higher anti-inflammatory activity than diclofenac sodium and ginger alone. With this initial study, research work could be extended further; therefore, the particular pharmacological action for the combination of ginger with diclofenac sodium could be discovered.

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