Bacterial Diversity of Lonar Soda Lake of India

A novel Gram-stain-positive, rod-shaped, motile, endospore-forming and proteolytic bacterial strain, SPT, was isolated from Lonar soda lake, in India. On the basis of 16S rRNA gene sequence analysis it was identified as belonging to the class Firmibacteria and was most closely related to Bacillus cellulosilyticus DSM 2522T (96.7 %) and other members of the genus Bacillus ( < 95.9 %). Strain SPT was catalase- and oxidase-positive. The cell-wall peptidoglycan of strain SPT contained meso-diaminopimelic acid. Polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three phospholipids, two aminolipids and two unknown lipids. The predominant isoprenoid quinone was MK-7. Anteiso-C15 : 0 (26.8 %) was the predominant fatty acid and significant proportions (>5 %) of iso-C15 : 0 (20.9 %), C16 : 1ω7c alcohol (6.3 %), iso-C16 : 0 (6.3 %) and anteiso-C17 : 0 (5.3 %) were also detected in strain SPT. The DNA G+C content of strain SPT was 38.9 mol%. The results of phylogenetic, chemotaxonomic and biochemical tests allowed a clear differentiation of strain SPT from all other members of the genus Bacillus. Strain SPT represents a novel member of the genus Bacillus, for which the name Bacillus caseinilyticus sp. nov. is proposed. The type strain is SPT ( = MCC 2612T = JCM 30246T).

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Spatio-temporal resolution of taxonomic and functional microbiome of Lonar soda lake of India reveals metabolic potential for bioremediation

Chemosphere ◽

10.1016/j.chemosphere.2020.128574 ◽

2021 ◽

Vol 264 ◽

pp. 128574

Author(s):

Jaya Chakraborty ◽

Vinay Rajput ◽

Vibhavari Sapkale ◽

Sanjay Kamble ◽

Mahesh Dharne

Keyword(s):

Temporal Resolution ◽

Soda Lake ◽

Metabolic Potential ◽

Lonar Soda Lake ◽

Spatio Temporal

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Molecular analyses of microbial diversity associated with the Lonar soda lake in India: An impact crater in a basalt area

Research in Microbiology ◽

10.1016/j.resmic.2006.08.005 ◽

2006 ◽

Vol 157 (10) ◽

pp. 928-937 ◽

Cited By ~ 64

Author(s):

Aijaz Ahmad Wani ◽

Venkata Prasad Surakasi ◽

Jay Siddharth ◽

Raamesh Gowri Raghavan ◽

Milind S. Patole ◽

...

Keyword(s):

Microbial Diversity ◽

Impact Crater ◽

Soda Lake ◽

Molecular Analyses ◽

Lonar Soda Lake

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Microbial community of soda Lake Van as obtained from direct and enriched water, sediment and fish samples

Scientific Reports ◽

10.1038/s41598-021-97980-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Esra Ersoy Omeroglu ◽

Mert Sudagidan ◽

Mediha Nur Zafer Yurt ◽

Behiye Busra Tasbasi ◽

Elif Esma Acar ◽

...

Keyword(s):

Bacterial Diversity ◽

Water Samples ◽

Soda Lakes ◽

Soda Lake ◽

Rrna Gene ◽

Lake Van ◽

Sediment Samples ◽

Fish Samples ◽

High Level ◽

Generation Sequencing

AbstractSoda lakes are saline and alkaline ecosystems that are considered to have existed since the first geological records of the world. These lakes support the growth of ecologically and economically important microorganisms due to their unique geochemistry. Microbiota members of lakes are valuable models to study the link between community structure and abiotic parameters such as pH and salinity. Lake Van is the largest endroheic lake and in this study, bacterial diversity of lake water, sediment, and pearl mullet (inci kefali; Alburnus tarichi), an endemic species of fish which are collected from different points of the lake, are studied directly and investigated meticulously using a metabarcoding approach after pre-enrichment. Bacterial community structures were identified using Next Generation Sequencing of the 16S rRNA gene. The analysis revealed that the samples of Lake Van contain high level of bacterial diversity. Direct water samples were dominated by Proteobacteria, Cyanobacteria, and Bacteroidota, on the other hand, pre-enriched water samples were dominated by Proteobacteria and Firmicutes at phylum-level. In direct sediment samples Proteobacteria, whereas in pre-enriched sediment samples Firmicutes and Proteobacteria were determined at highest level. Pre-enriched fish samples were dominated by Proteobacteria and Firmicutes at phylum-level. In this study, microbiota members of Lake Van were identified by taxonomic analysis.

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