Thermophilic Anaerobic Digestion of Arundo donax cv. Lvzhou No. 1 for Biogas Production: Structure and Functional Analysis of Microbial Communities

2020 ◽  
Vol 13 (3) ◽  
pp. 866-877 ◽  
Author(s):  
Yating Lei ◽  
Chao Xie ◽  
Xiaoxi Wang ◽  
Zhiyi Fang ◽  
Ying Huang ◽  
...  
2020 ◽  
Vol 96 (10) ◽  
Author(s):  
Francesca Bandini ◽  
Chiara Misci ◽  
Eren Taskin ◽  
Pier Sandro Cocconcelli ◽  
Edoardo Puglisi

ABSTRACT The development of biopolymers has raised issues about their recalcitrance in the environment. Their disposal is mainly carried out with the organic fraction of municipal solid waste (OFMSW) through thermophilic anaerobic digestion and aerobic composting, bioprocesses aimed at turning organic matter into biogas and compost. However, the effects of biopolymers on OFMSW treatment, on the final compost and on the microbial communities involved are partly unexplored. In this study, the OFMSW treatment was reproduced on a laboratory-scale respecting real plant conditions and testing the impacts of mixing polylactic acid (PLA) and starch-based bioplastic (SBB) separately. The dynamics of bacterial, archaeal and fungal communities during the process was screened by high-throughput sequencing (HTS) of phylogenetic amplicons. Starch-based bioplastic showed a minor and heterogeneous microbial diversity between the anaerobic and aerobic phases. Contrariwise, PLA treatment resulted in wider and more diverse bacterial and fungal communities for the compost and the aerobic biofilm. Since the biodiversity in compost may play a crucial role in its stability and safety, the modulation of environmental microbial communities induced by higher concentrations of PLA in OFMSW treatment can pose relevant issues.


2002 ◽  
Vol 46 (4-5) ◽  
pp. 447-453 ◽  
Author(s):  
J. Zábranská ◽  
M. Dohányos ◽  
P. Jeníček ◽  
P. Zaplatílková ◽  
J. Kutil

Thermophilic anaerobic digestion of sewage sludge has been successfully operated in full-scale tanks almost three years. The higher loading capacity and specific biogas production rate in comparison with mesophilic digestion was proved. Thermophilic anaerobic sludge is also more resistant against foaming problems. Biogas from thermophilic tanks contains less hydrogen sulphide and other malodorous substances. Pathogens removal rate is apparently more efficient in the thermophilic process.


2007 ◽  
Vol 131 (2) ◽  
pp. S176 ◽  
Author(s):  
Masayuki Onodera ◽  
Tadashi Ootsu ◽  
Eiichi Sato ◽  
Masanobu Kusakabe ◽  
Satoshi Takesono ◽  
...  

2015 ◽  
Vol 10 (4) ◽  
pp. 192 ◽  
Author(s):  
Federico Dragoni ◽  
Giorgio Ragaglini ◽  
Elisa Corneli ◽  
Nicoletta Nassi o Di Nasso ◽  
Cristiano Tozzini ◽  
...  

Aiming to improve the sustainability of biogas supply chains, the research for alternative feedstocks is a key issue and giant reed (<em>Arundo donax</em> L.) is a promising no-food crop to be used in anaerobic digestion. In fact, giant reed is a perennial species characterised by low nutrient requirements and is able to provide promising biogas yields. Its suitability for anaerobic digestion is influenced by harvest time, since plant characteristics vary noticeably along the season. Moreover, ensiling is a storage technique that can assure a good preservation of the biomass over time, but also influence the methane yields. Therefore, the aim of this study was to assess the suitability for biogas production of giant reed silage, according to different cutting regimes, and to evaluate the efficiency in saving land and nitrogen for fuelling biogas plants, in comparison with maize and two sorghum varieties. Methane yields per hectare (Nm<sup>3</sup> CH<sub>4</sub> ha<sup>–1</sup>) were determined by multiplying the biochemical methane potential of each substrate by the aboveground biomass of the corresponding crop. The land use coefficient (LU), namely the land needed to fuel one kW power (ha kWe<sup>–1</sup>), was calculated from the estimated methane yields per hectare. Finally, nitrogen utilisation efficiency (NUtE), which is the ratio between the estimated methane yield and the nitrogen uptake per hectare (Nm<sup>3</sup> CH<sub>4</sub> kgN<sup>–1</sup>), was determined for each crop species and according to the harvest time and frequency of giant reed. Overall, a good suitability for ensiling was observed in giant reed. When harvested in September, the crop yielded about 9900 Nm<sup>3</sup> CH<sub>4</sub> ha<sup>–1</sup>, while in double harvest systems biomethane was about 12,000 Nm<sup>3</sup> CH<sub>4</sub> ha<sup>–1</sup>, +35% and +70% than maize and sorghum respectively. Moreover, giant reed under double harvest management was the most land-conservative option, as LU was about 0.22 ha kWe<sup>–1</sup>, while in annual crops it was about 0.35 ha kWe<sup>–1</sup>. The higher NUtE was observed in single harvests (up to 64 Nm<sup>3</sup> CH<sub>4</sub> kgN<sup>–1</sup>), while double harvests showed remarkably lower values, averaging 48 Nm<sup>3</sup> CH<sub>4</sub> kgN<sup>–1</sup>. Annual crops were less efficient, since NUtE ranged from 28 Nm<sup>3</sup> CH<sub>4</sub> kgN<sup>–1</sup> (maize) to 40 Nm<sup>3</sup> CH<sub>4</sub> kgN<sup>–1</sup> (fibre sorghum). In conclusion, giant reed can be an alternative for biogas making, potentially providing land and nitrogen savings compared with conventional annual crops.


2020 ◽  
Vol 10 (7) ◽  
pp. 2412
Author(s):  
Slawomir Kasinski

The objective of this study was to investigate the effect of process temperature on semi-continuous anaerobic digestion of the organic fraction separated during autoclaving of municipal waste. Tests were carried out in reactors with full mixing. Biogas production was higher in thermophilic conditions than in mesophilic conditions (0.92 L/g volatile solids at 55 °C vs. 0.42 L/g volatile solids at 37 °C, respectively). The resulting methane yields were 0.25-0.32 L CH4/g VS and 0.56–0.70 L CH4/g VS in mesophilic and thermophilic conditions, respectively. In both variants, the methane share was over 70% v/v. This work also discusses the potential impact of Maillard compounds on the efficiency of the fermentation process, which were probably produced during the process of autoclaving of municipal waste. These results indicate that, after autoclaving, the organic fraction of municipal waste can be an effective substrate for anaerobic digestion in thermophilic conditions.


2013 ◽  
Vol 133 ◽  
pp. 23-30 ◽  
Author(s):  
Nilmini Beneragama ◽  
Suraju A. Lateef ◽  
Masahiro Iwasaki ◽  
Takaki Yamashiro ◽  
Kazutaka Umetsu

Energies ◽  
2021 ◽  
Vol 14 (3) ◽  
pp. 630
Author(s):  
Ciro Vasmara ◽  
Stefano Cianchetta ◽  
Rosa Marchetti ◽  
Enrico Ceotto ◽  
Stefania Galletti

The biogas production through the anaerobic digestion (AD) of giant reed (Arundo donax L.) biomass has received increasing attention. However, due to the presence of lignin, a low CH4 yield can be obtained. Aiming to improve the CH4 yield from giant reed biomass, the effectiveness of a thermo-chemical pre-treatment based on KOH was evaluated in this paper. The usefulness of a washing step before the AD was also assessed. The pre-treatment led to a specific CH4 yield up to 232 mL CH4 g−1 VS which was 21% higher than that from untreated biomass; the maximum daily rate of production was improved by 42%, AD duration was reduced by 10%, and CH4 concentration in the biogas was increased by 23%. On the contrary, the washing step did not improve the AD process. Besides, washing away the liquid fraction led to biomass losses, reducing the overall CH4 production. The use of a KOH-based pre-treatment appears as a good option for enhancing the AD of giant reed, also presenting potential environmental and agronomical benefits, like the avoidance of salty wastewater production and the likely improvement of the digestate quality, due to its enriched K content.


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