Effects of Bulk Tank Milk, Waste Milk, and Pasteurized Waste Milk on the Intake, Ruminal Parameters, Blood Parameters, Health, and Performance of Dairy Calves

The aim of this study was to evaluate the effects of bulk tank milk (BTM), WM, and PWM on the intake, ruminal parameters, blood parameters, health, and performance of dairy calves. Forty-five male crossbred dairy calves (Gyr × Holstein) were used. On their fourth day of age, animals were grouped according to body weight, serum protein levels, and genetic composition. Three treatments were assessed: BTM (n = 15), WM from cows in antibiotic treatment (n = 15), and PWM via high-temperature, short-time pasteurization (72–74 °C for 16 s) (n = 15). During the experimental period (from 4 to 60 d of age), animals were fed 6 L of milk/d, divided into two equal meals. Water and concentrate were provided ad libitum. Daily measurements were made for milk, concentrate, and water intakes, as well as for fecal and respiratory scores. Rumen fluid and blood were sampled weekly. The following parameters were evaluated: volatile fatty acids (VFAs), pH and ammonia-N in rumen fluid, and β-hydroxybutyrate (BHB) and glucose in blood. Animals were weighed at birth, 4 d of age, and weekly up to 60 d of age. At the end of the experimental period (60 ± 1 d), all animals were euthanized for pulmonary evaluation. The randomized complete design with an interaction between treatment and week was the experimental method of choice for testing the hypothesis of the treatment’s effect on all evaluated outcomes. Animals in the BTM treatment had higher milk dry matter intake (DMI), followed by WM and PWM calves. Concentrate DMI was lower for BTM in comparison to WM and PWM calves. However, total DMI showed no significant differences between treatments. The rumen fluid from calves receiving PWM had higher concentrations of acetate and propionate than that of BTM and WM animals. No differences were observed between treatments for blood glucose and BHB concentrations. Health parameters (fecal and respiratory scores) and pneumonia occurrence showed no significant difference between treatments. No differences were observed for average daily gain (ADG) or body growth. Feeding WM and PWM did not show significant negative effects on the intake, ruminal parameters, blood parameters, health, or performance of dairy calves.

Strategic marketing to strengthen dairy production

Microenterprises that produce dairy products face problems of unfair competition, unfavorable government regulations, and under-utilization of milk derivatives. This work focuses its objective on analyzing and developing strategic marketing for the strengthening of dairy production; For this, the contributions of marketing and its relationship with dairy production are enunciated and the marketing strategies of applied products are analyzed. Through a qualitative approach, with quantitative data, in the light of a descriptive, cross-sectional method, the methodological process is developed, through which results are obtained that show the need to propose product strategies and take advantage of serum waste milk. In this sense, the elaboration of products derived from whey is proposed for their subsequent commercialization. As a conclusion, small economy companies cannot afford investments of more than 800,000.00 USD; therefore, they require alternatives such as associativity. 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Prevalence and Risk Factors for ESBL/AmpC-E. coli in Pre-Weaned Dairy Calves on Dairy Farms in Germany

The objectives of this study were to ascertain the fecal ESBL/AmpC-E. coli prevalence and to detect risk factors for their occurrence in young pre-weaned calves and their dams on large dairy farms in Germany. From 2018–2019 we investigated 2816 individual fecal samples from pre-weaned dairy calves and their dams, representing seventy-two farms (mean = 667 milking cows) from eight German federal states. To assess possible risk factors associated with ESBL/AmpC-E. coli prevalence in calves and dams, a questionnaire was performed, collecting management data. We observed an ESBL/AmpC-E. coli prevalence of 63.5% (95% CI: 57.4–69.5) among the sampled calves and 18.0% (95% CI: 12.5–23.5) among the dams. On all farms, at least one positive sample was obtained. To date, this is the highest ESBL/AmpC-E. coli prevalence observed in dairy herds in Europe. Feeding with waste milk was identified as a significant risk factor for a high prevalence of ESBL/AmpC-E. coli in calves. Many calves at large dairies in Germany are fed with waste milk due to the large amounts generated as a result of antibiotic dry-off routines and mastitis treatment with antibiotics. Other notable risk factors for high ESBL/AmpC-E. coli in calves were the general fitness/health of dams and calves, and the quality of farm hygiene. Taken together, these findings suggest that new or improved approaches to animal health management, for example, antibiotic dry cow management (selective dry cow therapy) and mastitis treatment (high self-recovery), as well as farm hygiene, should be researched and implemented.

Effects of waste milk on growth performance, immunity, and gut health of dairy calves

Background Liquid feed are the major nutrient source that can have a significant impact on the growth and development of immune system of calves before weaning. Waste milk containing antibiotic residue has been produced because of the continuous expansion of dairy farms. In order to reduce economic loss and prevent environmental pollution, most farms seem waste milk as one of the calves’ liquid feeds. However, there is limited information to report the effects of waste milk on growth performance, especially immunity function of calves. Thus, the objective of this study was to investigate the effects of waste milk on growth, immunity and gut health of dairy calves. Results Feeding WM improved hip width, hip height, heart girth, final body weight, and feed efficiency of dairy calves compared to MR. Plasma concentrations of IgA, IgM, IgG and IL-10 were higher and TNF-α was lower in WM group. In addition, treatment and time interactively affected plasma concentrations of IgG and IL-2, which increased and decreased in WM group but decreased and increased in MR group, respectively, from 49 to 70 d of age. There was no difference in diarrhea case and average days of diarrhea among treatments. Difference in fecal microbiota was observed between MR and WM groups only at 49 d of age. Analysis of differential abundance showed that the increase in the relative abundance of Prevotellaceae NK3B31 group and the decrease in that of Bacteroides was higher in WM than MM group from 49 to 70 d of age. Conclusions WM had beneficial effects on growth performance and did not affect health statue, which might be explained by enhanced immune function modulated by fecal microbiota.

Feeding Pre-weaned Calves With Waste Milk Containing Antibiotic Residues Is Related to a Higher Incidence of Diarrhea and Alterations in the Fecal Microbiota

The cows receiving antibiotics for intra-mammary infection (IMI) produce milk that cannot be marketed. This is considered waste milk (WM), and a convenient option for farmers is using it as calf food. However, adding to the risk of selecting resistant bacteria, residual antibiotics might interfere with the gut microbiome development and influence gastrointestinal health. We assessed the longitudinal effect of unpasteurized WM containing residual cefalexin on calf intestinal health and fecal microbiota in an 8-week trial. After 3 days of colostrum, six calves received WM and six calves received bulk tank milk (BM) for 2 weeks. For the following 6 weeks, all 12 calves received milk substitute and starter feed. Every week for the first 2 weeks and every 2 weeks for the remaining 6 weeks, we subjected all calves to clinical examination and collected rectal swabs for investigating the fecal microbiota composition. Most WM calves had diarrhea episodes in the first 2 weeks of the trial (5/6 WM and 1/6 BM), and their body weight was significantly lower than that of BM calves. Based on 16S rRNA gene analysis, WM calves had a lower fecal microbiota alpha diversity than that in BM calves, with the lowest p-value at Wk4 (p < 0.02), 2 weeks after exposure to WM. The fecal microbiota beta diversity of the two calf groups was also significantly different at Wk4 (p < 0.05). Numerous significant differences were present in the fecal microbiota taxonomy of WM and BM calves in terms of relative normalized operational taxonomic unit (OTU) levels, affecting five phyla, seven classes, eight orders, 19 families, and 47 genera. At the end of the trial, when 6 weeks had passed since exposure to WM, the phyla Bacteroidetes, Firmicutes, and Saccharibacteria were lower, while Chlamydiae were higher in WM calves. Notably, WM calves showed a decrease in beneficial taxa such as Faecalibacterium, with a concomitant increase in potential pathogens such as Campylobacter, Pseudomonas, and Chlamydophila spp. In conclusion, feeding pre-weaned calves with unpasteurized WM containing antibiotics is related to a higher incidence of neonatal diarrhea and leads to significant changes in the fecal microbiota composition, further discouraging this practice in spite of its short-term economic advantages.

PREPARATION OF WATER RESISTANT GLUE FROM MILK PLASTIC

In this process of extracting Milk Plastic Water Resistant Glue from the casein 3 type of milk soya milk, fresh cow milk and the pastured milk were used. Casein was extracted and vinegar was added while milk was getting heated. Casein was separated, and dried for 2-3 days. Milk Plastic that was obtained was heated with distilled water. A pinch of papain is added and made it hot for 4 hours and then 1N of NaOH is added and heated for few minutes. The collected sample is centrifuged at 12000 rpm and the glue was obtained. The glue is stored at -200C. 2 card board pieces was stacked together by using this glue and dipped in water to check the water resistance. It was stable for about 10 minutes and then it was loosened. It is little expensive comparing to other glues when it was did with pure milk while if it is done with waste milk product its cost will be low.

THE FOLIAR SPRAY APPLICATION OF SELECTED ORGANIC FERTILIZERS AND THEIR EFFECTS ON SELECTED PLANTS – A REVIEW

The organic fertilizer or manures like banana peel, coconut peat and waste milk tea (TW) are highly rich in nitrogen, potassium and phosphorus etc. (Vu, H. T., et al., 2018; Khan, M.Z., et al., 2019; Sial, T.A., et al., 2019). The sandy soil is one of the most severe conditions which negatively affect the growth of plant. The aim of the study is to report the foliar spray effect of milk tea waste extract, banana peel extract, coconut peat extract, on the growth of coriander and spinach plants. In this analysis different pots for various organic fertilizers were used to check their effect on plant growth and to investigate the improvement of biochemical properties of sandy soil (Vu, H. T., et al., 2018; Khan, M.Z., et al., 2019; Sial, T.A., et al., 2019).According to review, using various fertilizers growth parameters like plant height, numbers of leaves, shoot moisture content, NPK content present selected in plants can be compared. This review describes the best organic fertilizer among banana peel, coconut peat and waste milk tea and their effect on the growth parameters of coriander and spinach plant. KEYWORDS: Banana peel, Coconut peat, Waste milk tea, Fertilizer, Plants growth, Soil analysis.