A colorimetric lateral flow assay based on multiplex PCR for the rapid detection of viable Escherichia coli O157:H7 and Salmonella Typhimurium without enrichment

LWT ◽  
2021 ◽  
pp. 112242
Author(s):  
Jin-Hee Kim ◽  
Se-Wook Oh
Keyword(s):  
Escherichia Coli ◽  
Salmonella Typhimurium ◽  
Multiplex Pcr ◽  
Rapid Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Escherichia Coli O157

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

A gold nanoparticles-assisted multiplex PCR assay for simultaneous detection of Salmonella typhimurium, Listeria monocytogenes and Escherichia coli O157:H7

2020 ◽  
Vol 12 (2) ◽  
pp. 212-217 ◽  
Author(s):  
Juan Du ◽  
Shujing Wu ◽  
Liyuan Niu ◽  
Junguang Li ◽  
Dianbo Zhao ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gold Nanoparticles ◽  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Multiplex Pcr ◽  
Simultaneous Detection ◽  
Escherichia Coli O157 ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Pcr Product

Unfunctionalized flower-shaped AuNPs is used as colorimetric sensor for PCR product detection by naked eyes.

scholarly journals Rapid Detection of Escherichia coli O157 and Shiga Toxins by Lateral Flow Immunoassays

Toxins ◽  
2016 ◽  
Vol 8 (4) ◽  
pp. 92 ◽  
Author(s):  
Jinliang Wang ◽  
Robab Katani ◽  
Lingling Li ◽  
Narasimha Hegde ◽  
Elisabeth Roberts ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Lateral Flow ◽  
Escherichia Coli O157 ◽  
Shiga Toxins

scholarly journals Nanozyme-based lateral flow assay for the sensitive detection of Escherichia coli O157:H7 in milk

2018 ◽  
Vol 101 (7) ◽  
pp. 5770-5779 ◽  
Author(s):  
Jiaojiao Han ◽  
Lei Zhang ◽  
Liming Hu ◽  
Keyu Xing ◽  
Xuefei Lu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Sensitive Detection ◽  
Escherichia Coli O157
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