Laminar Shear Stress Promotes Nicotine-Induced Inflammation and Hemostatic Expression in Human Endothelial Cells

2016 ◽  
Vol 9 (3) ◽  
pp. 466-477 ◽  
Author(s):  
Yu-Hsiang Lee ◽  
Chi-Chung Lee ◽  
Chien-Hsun Huang ◽  
Feng-Ming Ho
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Laminar Shear Stress ◽  
Human Endothelial Cells ◽  
Laminar Shear

scholarly journals Primary cilia of human endothelial cells disassemble under laminar shear stress

2004 ◽  
Vol 164 (6) ◽  
pp. 811-817 ◽  
Author(s):  
Carlo Iomini ◽  
Karla Tejada ◽  
Wenjun Mo ◽  
Heikki Vaananen ◽  
Gianni Piperno
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Primary Cilia ◽  
Umbilical Vein ◽  
Intraflagellar Transport ◽  
Mechanical Stimuli ◽  
Laminar Shear Stress ◽  
Human Endothelial Cells ◽  
Umbilical Vein Endothelial Cells ◽  
Laminar Shear

We identified primary cilia and centrosomes in cultured human umbilical vein endothelial cells (HUVEC) by antibodies to acetyl-α-tubulin and capillary morphogenesis gene-1 product (CMG-1), a human homologue of the intraflagellar transport (IFT) protein IFT-71 in Chlamydomonas. CMG-1 was present in particles along primary cilia of HUVEC at interphase and around the oldest basal body/centriole at interphase and mitosis. To study the response of primary cilia and centrosomes to mechanical stimuli, we exposed cultured HUVEC to laminar shear stress (LSS). Under LSS, all primary cilia disassembled, and centrosomes were deprived of CMG-1. We conclude that the exposure to LSS ends the IFT in cultured endothelial cells.

A Promoter Polymorphism Regulates NFKB1 Gene Transactivity in Human Endothelial Cells under Laminar Shear Stress

2006 ◽  
Vol 38 (Supplement) ◽  
pp. S4
Author(s):  
Joon Y. Park ◽  
Iain K. Farrance ◽  
Hanjoong Jo ◽  
Steven R. Brant ◽  
Stephen M. Roth ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Promoter Polymorphism ◽  
Laminar Shear Stress ◽  
Human Endothelial Cells ◽  
Laminar Shear

AXL ACTIVATION MEDIATES LAMINAR SHEAR STRESS ANTIAPOPTOTIC EFFECTS IN HUMAN ENDOTHELIAL CELLS

2004 ◽  
Vol 13 (3) ◽  
pp. 194
Author(s):  
Daniela D'Arcangelo ◽  
Valeria Ambrosino ◽  
Gianluca Ragone ◽  
Maria Giannuzzo ◽  
Maurizio C Capogrossi ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Laminar Shear Stress ◽  
Human Endothelial Cells ◽  
Laminar Shear

scholarly journals Axl receptor activation mediates laminar shear stress anti-apoptotic effects in human endothelial cells

2006 ◽  
Vol 71 (4) ◽  
pp. 754-763 ◽  
Author(s):  
D DARCANGELO ◽  
V AMBROSINO ◽  
M GIANNUZZO ◽  
C GAETANO ◽  
M CAPOGROSSI
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Receptor Activation ◽  
Laminar Shear Stress ◽  
Human Endothelial Cells ◽  
Apoptotic Effects ◽  
Laminar Shear

Endothelin receptor B-mediated induction of c-jun and AP-1 in response to shear stress in human endothelial cellsThis article is one of a selection of papers published in the special issue (part 2 of 2) on Forefronts in Endothelin.

2008 ◽  
Vol 86 (8) ◽  
pp. 499-504 ◽  
Author(s):  
H. Morawietz ◽  
A.H. Wagner ◽  
M. Hecker ◽  
W. Goettsch
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Endothelin Receptor ◽  
Laminar Shear Stress ◽  
Human Endothelial Cells ◽  
Endothelin 1 ◽  
Subtype B ◽  
Short Term Exposure ◽  
Stress Dependent ◽  
Laminar Shear

In vivo, endothelial cells are constantly exposed to shear stress by flowing blood. Short-term exposure of endothelial cells to shear stress has been shown to induce endothelin-1 release. It is currently unknown, however, whether this shear stress-dependent endothelin-1 release affects the expression and activity of transcription factors. In this study, primary cultures of human endothelial cells from the umbilical vein were exposed to laminar shear stress in a cone-and-plate viscometer. Laminar shear stress for 30 min induced a 2-fold increase in mRNA expression of c-jun , but not c-fos, in human endothelial cells. Blockade of endothelin receptor subtype B (ETB) with BQ788 prevented this shear stress-dependent induction of c-jun expression. The induction of c-jun by shear stress involved protein kinase C and endothelial NO synthase. In addition, exposure of endothelial cells to arterial laminar shear stress for 1 h increased the binding of transcription factor AP-1 to its consensus sequence by 1.7-fold in electrophoretic mobility shift assays. This induction was also mediated by an ETB-dependent pathway. Supershift analysis supports an AP-1 complex containing c-jun, but not c-fos, in human endothelial cells. In conclusion, our data suggest endothelin-1-mediated induction of c-jun expression and activation of AP-1 (possibly as a c-jun homodimer) by laminar shear stress in human endothelial cells.

Tissue plasminogen activator messenger RNA levels increase in cultured human endothelial cells exposed to laminar shear stress

1990 ◽  
Vol 143 (2) ◽  
pp. 364-371 ◽  
Author(s):  
S. L. Diamond ◽  
J. B. Sharefkin ◽  
C. Dieffenbach ◽  
K. Frasier-Scott ◽  
L. V. McIntire ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Messenger Rna ◽  
Laminar Shear Stress ◽  
Human Endothelial Cells ◽  
Tissue Plasminogen ◽  
Laminar Shear ◽  
Rna Levels

scholarly journals The Gene Expression of Human Endothelial Cells Is Modulated by Subendothelial Extracellular Matrix Proteins: Short-Term Response to Laminar Shear Stress

2014 ◽  
Vol 20 (15-16) ◽  
pp. 2253-2264 ◽  
Author(s):  
Jaroslav Chlupac ◽  
Elena Filova ◽  
Jana Havlikova ◽  
Roman Matejka ◽  
Tomas Riedel ◽  
...  
Keyword(s):  
Gene Expression ◽  
Extracellular Matrix ◽  
Endothelial Cells ◽  
Shear Stress ◽  
Matrix Proteins ◽  
Laminar Shear Stress ◽  
Human Endothelial Cells ◽  
Short Term ◽  
Laminar Shear ◽  
Term Response

IMPAIRMENT OF PAXILLIN FUNCTION INHIBITS LAMINAR SHEAR STRESS SENSING IN HUMAN ENDOTHELIAL CELLS

2004 ◽  
Vol 13 (3) ◽  
pp. 191
Author(s):  
Stefania Mattiussi ◽  
Fabio Martelli ◽  
Maurizio C. Capogrossi ◽  
Carlo Gaetano
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Laminar Shear Stress ◽  
Human Endothelial Cells ◽  
Stress Sensing ◽  
Laminar Shear

scholarly journals NO-mediated regulation of NAD(P)H oxidase by laminar shear stress in human endothelial cells

2006 ◽  
Vol 576 (2) ◽  
pp. 557-567 ◽  
Author(s):  
Nicole Duerrschmidt ◽  
Claudia Stielow ◽  
Gregor Muller ◽  
Patrick J. Pagano ◽  
Henning Morawietz
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Laminar Shear Stress ◽  
Human Endothelial Cells ◽  
Laminar Shear

Laminar shear stress promotes mitochondrial homeostasis in endothelial cells

2018 ◽  
Vol 233 (6) ◽  
pp. 5058-5069 ◽  
Author(s):  
Li-Hong Wu ◽  
Hao-Chun Chang ◽  
Pei-Ching Ting ◽  
Danny L. Wang
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Laminar Shear Stress ◽  
Mitochondrial Homeostasis ◽  
Laminar Shear
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