scholarly journals A High Throughput Method for Generating Dihaploids from Tetraploid Potato

2021 ◽  
Vol 98 (4) ◽  
pp. 304-314
Author(s):  
James S. Busse ◽  
Shelley H. Jansky ◽  
Husain I. Agha ◽  
Cari A. Schmitz Carley ◽  
Laura M. Shannon ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Solanum Tuberosum ◽  
High Throughput ◽  
Production Method ◽  
Promising Method ◽  
Breeding Lines ◽  
Tetraploid Potato ◽  
Cultivar Development ◽  
High Throughput Method ◽  
The Cost

AbstractThere is a worldwide effort to increase the efficiency of potato (Solanum tuberosum L.) cultivar development by using inbred diploid breeding lines. This activity is impeded by the cost and effort required to produce dihaploids from cultivated tetraploid potatoes. We developed a high throughput dihaploid production method based on the 60-year-old method of Peloquin and Hougas. Red Norland inflorescences from commercial fields were transferred to greenhouses. As buds developed, pollen from the dihaploid inducer IVP 101 was applied systematically to thousands of stigmas per trial. Berries were harvested 21 days after pollination. Seeds of putative dihaploids lacking a seed spot marker were retained and ploidy was confirmed using flow cytometry. We recovered 23 dihaploids from 21,651 pollinations. This is a promising method for systematically carrying out thousands of pollinations since the cost of field-grown flowers is dramatically less than that of greenhouse-grown flowers.

A Note on Development of a Low-cost and High-throughput SSR-based Genotyping Method in Peanut (Arachis hypogaea L.)

2011 ◽  
Vol 38 (2) ◽  
pp. 122-127 ◽  
Author(s):  
Jake Fountain ◽  
Hongde Qin ◽  
Charles Chen ◽  
Phat Dang ◽  
Ming Li Wang ◽  
...  
Keyword(s):  
High Throughput ◽  
Silver Staining ◽  
Low Cost ◽  
Digital Camera ◽  
Polyacrylamide Gel ◽  
Cultivar Development ◽  
Arachis Hypogaea L ◽  
Data Points ◽  
Breeding Selection ◽  
The Cost

ABSTRACT Peanut cultivar development has been dominated by conventional breeding methods, which have and will continue to play an important role. Applications of marker-assisted selection (MAS) have been used in peanut breeding selection but the cost of genotyping is still a considerable factor. The objective of this study was to introduce a simple, low-cost, and high-throughput protocol for peanut community. The developed system was based on a smaller (10.5 cm in length) polyacrylamide gel size system to separate PCR amplified DNA fragments and silver staining to visualize the bands. This system is very easy to operate, having one electrophoresis unit holds two vertical 52-sample gels, and the cost for purchasing the unit is less than $200. For instance, the electrophoresis runs about 1 hr and 40 min at 180 V for 9% polyacrylamide gel to separate small to medium sized DNA bands (< 500 bp) or 1 hr and 20 min at 160 V for 6% polyacrylamide gel preferably for larger band separation (≥ 500 bp), but the gel concentrations and running times could be adjusted according to the polymorphic banding patterns and sizes to mitigate the drawback of this system of small gel-size. The silver staining takes about 30 min. After staining, the gels are placed on a light-box for genotype scoring and then photographed using a digital camera. The cost per gel is estimated at $0.54 and the cost for silver staining is estimated at $0.37. Therefore, the cost could be as low as $0.018 per data point, excluding PCR reaction and DNA preparation cost. A scientist has the potential to generate over 1,200 data points per day. This method has been used in the construction of a peanut genetic linkage map and QTL studies in our laboratory in conjunction with other methods.

scholarly journals A high throughput method for quantifying number and size distribution of Arabidopsis seeds using large particle flow cytometry

Plant Methods ◽  
2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Alejandro Morales ◽  
J. Teapal ◽  
J. M. H. Ammerlaan ◽  
X. Yin ◽  
J. B. Evers ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Size Distribution ◽  
High Throughput ◽  
Large Particle ◽  
Particle Flow ◽  
High Throughput Method

scholarly journals A high-throughput method for detection of DNA in chloroplasts using flow cytometry

Plant Methods ◽  
2007 ◽  
Vol 3 (1) ◽  
pp. 5 ◽  
Author(s):  
Beth A Rowan ◽  
Delene J Oldenburg ◽  
Arnold J Bendich
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
High Throughput Method

A Fluorescent High Throughput Method To Identify Potential Skin Sensitizers

Planta Medica ◽  
2016 ◽  
Vol 82 (05) ◽  
Author(s):  
C Avonto ◽  
AG Chittiboyina ◽  
D Rua ◽  
IA Khan
Keyword(s):  
High Throughput ◽  
High Throughput Method

A deep learning-based concept for high throughput image flow cytometry

2021 ◽  
Vol 118 (12) ◽  
pp. 123701
Author(s):  
Julie Martin-Wortham ◽  
Steffen M. Recktenwald ◽  
Marcelle G. M. Lopes ◽  
Lars Kaestner ◽  
Christian Wagner ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Deep Learning ◽  
High Throughput ◽  
Image Flow

scholarly journals High Throughput Centrifugal Electrospinning of Polyacrylonitrile Nanofibers for Carbon Fiber Nonwovens

Polymers ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1313
Author(s):  
Andreas Hoffmann ◽  
Alexander J. C. Kuehne
Keyword(s):  
High Throughput ◽  
Carbon Nanofiber ◽  
Fiber Diameter ◽  
Carbon Precursor ◽  
Production Methods ◽  
High Throughput Method ◽  
Radial Forces ◽  
Fiber Deposition ◽  
The Impact ◽  
Pan Fibers

Carbon nanofiber nonwovens are promising materials for electrode or filtration applications; however, their utilization is obviated by a lack of high throughput production methods. In this study, we utilize a highly effective high-throughput method for the fabrication of polyacrylonitrile (PAN) nanofibers as a nonwoven on a dedicated substrate. The method employs rotational-, air pressure- and electrostatic forces to produce fibers from the inner edge of a rotating bell towards a flat collector. We investigate the impact of all above-mentioned forces on the fiber diameter, morphology, and bundling of the carbon-precursor PAN fibers. The interplay of radial forces with collector-facing forces has an influence on the uniformity of fiber deposition. Finally, the obtained PAN nanofibers are converted to carbon nonwovens by thermal treatment.

scholarly journals High-throughput multiparametric imaging flow cytometry: toward diffraction-limited sub-cellular detection and monitoring of sub-cellular processes

Cell Reports ◽  
2021 ◽  
Vol 34 (10) ◽  
pp. 108824
Author(s):  
Gregor Holzner ◽  
Bogdan Mateescu ◽  
Daniel van Leeuwen ◽  
Gea Cereghetti ◽  
Reinhard Dechant ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Multiparametric Imaging ◽  
Cellular Processes ◽  
Imaging Flow Cytometry
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