In vitro Regeneration of Aconitum balfourii Stapf: A Rare Medicinal Herb from Himalayan Alpine Through Root Explants

2012 ◽  
Vol 6 (4) ◽  
pp. 318-325 ◽  
Author(s):  
A.K. Gaur ◽  
Eti Sharma ◽  
Khushhaal Gaur ◽  
Himanshu Punetha
Keyword(s):  
In Vitro Regeneration ◽  
Medicinal Herb ◽  
Root Explants

scholarly journals Natural Variation in Plant Pluripotency and Regeneration

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1261
Author(s):  
Robin Lardon ◽  
Danny Geelen
Keyword(s):  
De Novo ◽  
Shoot Organogenesis ◽  
In Vitro Regeneration ◽  
Relative Role ◽  
Root Explants ◽  
Experimental Systems ◽  
Complex Process ◽  
Insight Into ◽  
Molecular Framework

Plant regeneration is essential for survival upon wounding and is, hence, considered to be a strong natural selective trait. The capacity of plant tissues to regenerate in vitro, however, varies substantially between and within species and depends on the applied incubation conditions. Insight into the genetic factors underlying this variation may help to improve numerous biotechnological applications that exploit in vitro regeneration. Here, we review the state of the art on the molecular framework of de novo shoot organogenesis from root explants in Arabidopsis, which is a complex process controlled by multiple quantitative trait loci of various effect sizes. Two types of factors are distinguished that contribute to natural regenerative variation: master regulators that are conserved in all experimental systems (e.g., WUSCHEL and related homeobox genes) and conditional regulators whose relative role depends on the explant and the incubation settings. We further elaborate on epigenetic variation and protocol variables that likely contribute to differential explant responsivity within species and conclude that in vitro shoot organogenesis occurs at the intersection between (epi) genetics, endogenous hormone levels, and environmental influences.

scholarly journals Indirect in vitro Regeneration of Viola canescens Wall. ex, Roxb. by using Leaf Calli

2018 ◽  
Vol 28 (2) ◽  
pp. 215-222 ◽  
Author(s):  
Arun Kumar Khajuria ◽  
NS Bisht
Keyword(s):  
Plant Regeneration ◽  
Natural Population ◽  
Plant Tissue ◽  
In Vitro Regeneration ◽  
Soil Condition ◽  
Medicinal Herb ◽  
Growth Chamber ◽  
Half Strength ◽  
Number Of Shoots

An efficient indirect plant regeneration protocol was developed for Viola canescens, an important medicinal herb used in broad spectra of diseases in number of folk medicines since aeon. Excessive use of this plant without any rehabilitating measure has led to decline its natural population. Present investigation reports the use of zeatin to regenerate the plant from the callus on MS following its acclimatization on the soil condition. Calli of the plant responded positively to zeatin and maximum number of shoots 13.07 ± 2.01 were obtained when 9.12 μM concentration of zeatin was used. Regenerated shoots were subsequently rooted with IBA on MS and half strength MS and showed maximum number of roots 14.13 ± 1.64 after 60 days when medium was fortified with 4.92 μM IBA, followed by transferring them to soil condition, acclimatization of the plantlet was carried in growth chamber and then finally to the field for their survival where it showed 80% survival. Plant Tissue Cult. & Biotech. 28(2): 215-222, 2018 (December)

scholarly journals Response of root explants to in vitro cultivation of marketable garlic cultivars

2013 ◽  
Vol 31 (1) ◽  
pp. 80-85 ◽  
Author(s):  
Danielle C Scotton ◽  
Vagner Augusto Benedito ◽  
Jeanne B de Molfetta ◽  
Benedita Inês FP Rodrigues ◽  
Augusto Tulmann-Neto ◽  
...  
Keyword(s):  
Disease Transmission ◽  
In Vitro Regeneration ◽  
Growth Conditions ◽  
Production Costs ◽  
In Vitro Cultivation ◽  
Ms Medium ◽  
Regeneration Medium ◽  
Root Explants ◽  
Breeding Programs

Garlic cultivars are sexually sterile under standard growth conditions, with direct implications for commercial production costs as well as breeding programs. Garlic is propagated commercially via bulblets, which facilitates disease transmission and virus load accumulation over vegetative generations. Tissue culture produces virus-free clones that are more productive, while keeping the desired traits of the cultivar. Consequently, this technique allows studies of garlic genetics as well as guarantees genetic conservation of varieties. We aimed at analyzing the in vitro regeneration of eight marketable cultivars of garlic using root segments as explants. For each genotype, bulblet-derived explants were isolated and introduced into MS medium supplemented with 2,4-D and 2-iP. Calli were transferred to MS medium supplemented with 8.8 mM BAP and 0.1 mM NAA (regeneration medium A), or with 4.6 mM kinetin alone (regeneration medium B). The calli were then evaluated for regeneration frequency after sixty days of in vitro cultivation. The noble cultivar 'Jonas' presented the highest rates of plant regeneration among the cultivars tested. The medium A, which contained auxin and cytokinin, induced the highest regeneration rates of all cultivars. The process described herein is simple, reproducible and can potentially be used as a tool in molecular breeding strategies for other marketable cultivars and genotypes of garlic.

scholarly journals An efficient in vitro regeneration system via callus from hypocotyl and root of Iris laevigata

2020 ◽  
Author(s):  
Fengyi Li ◽  
Lijuan Fan ◽  
Haijing Fu ◽  
Yujia Liu ◽  
Ling Wang
Keyword(s):  
In Vitro Regeneration ◽  
Genetic Material ◽  
Reproductive Capacity ◽  
Penicillin G ◽  
Induction Medium ◽  
Multiplication Rate ◽  
Regeneration System ◽  
Root Explants ◽  
2,4 Dichlorophenoxyacetic Acid

Abstract Background Iris laevigata is an ornamental plant with strong cold resistance. However, its low reproductive capacity limits its landscape applications. The I. laevigata wild genetic resources also need to be protected. In order to develop an effective regeneration system, the optimum agar concentration for the induction medium was determined. Two explants (hypocotyl and root) were then cultured on medium containing different concentrations of plant growth regulator (PGR). In addition, three antibiotics were evaluated for controlling endophyte contamination. Results The highest induction rate (75.00%) was obtained from hypocotyl explants on Murashige and Skoog salt mixture (MS) medium containing 6-benzylaminopurine (6-BA) 0.5 mg L-1 + 2,4-Dichlorophenoxyacetic acid (2,4-D) 1.0 mg L-1 + 1-naphthylacetic acid (NAA) 0.4 mg L-1. The medium containing 6-BA 0.5 mg L-1 + 2,4-D 0.5 mg L-1 + NAA 0.2 mg L-1 achieved the greatest multiplication rate (73.33%). Media containing indole-3-butyric acid (IBA) 0.5 mg L-1 + 6-BA 1.5 mg L-1 + NAA 1.0 mg L-1 achieved the highest differentiation rate (39.72%) for hypocotyl induced calli. Medium containing 6-BA 2.0 mg L-1 + NAA 0.4 mg L-1 + kinetin (KT) 1.0 mg L-1 resulted in the highest differentiation rate (49.52%) for root induced calli. One hundred mg L-1 penicillin G resulted in the optimal rate for reducing endophyte contamination. Conclusions The research determined the optimum PGR concentrations for inducting and multiplying I. laevigata calli from hypocotyl and root explants and a satisfactory means for control endophyte contamination. This research will result in the efficient and reliable reproduction of I. laevigata for landscape applications, genetic development of new Iris varieties, and preservation of the wild genetic material.

scholarly journals Tuberous Roots an Ideal System for High Frequency In vitro Regeneration in Plumbago rosea L.

1970 ◽  
Vol 20 (2) ◽  
pp. 203-209 ◽  
Author(s):  
Binoy Jose ◽  
K. Satheeshkumar
Keyword(s):  
High Frequency ◽  
In Vitro Regeneration ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Tuberous Root ◽  
Root Explants ◽  
Plumbago Rosea ◽  
Ideal System

Tuberous root explants of Plumbago rosea L. cultured in MS containing 3.5 mg/l BAP showed high frequency of shoot bud regeneration (62.33 ± 0.66; 0.26 ± 0.07 cm) within eight weeks. Subculture of shoot clumps (22.17 ± 0.90 shoots; 0.26 ± 0.07 cm) from the initial clump exhibited extensive rate of shoot multiplication (123.4 ± 1.029 shoots, 0.22 ± 0.01 cm) per each clump and produced a total of 369 shoots in 3.5 mg/l BAP in six weeks. Within two weeks of culture in basal liquid medium, shoot elongation (6.16 ± 0.14 cm) occurred and after three weeks  in MS solid medium supplemented with 0.1 mg/l IAA, rooting (14.7 ± 1.2) initiated on the shoots. Ninety six per cent of the rooted plants were established under the shade nethouse with regular watering.   Key words: Plumbago rosea, tuberous root, multiple shoots, High frequency   D.O.I. 10.3329/ptcb.v20i2.6915   Plant Tissue Cult. & Biotech. 20(2): 203-209, 2010 (December)

In vitro regeneration of watermelon seed segments

2015 ◽  
Vol 4 (8) ◽  
Author(s):  
M. Ameri ◽  
M. Lahouti ◽  
A. Bagheri ◽  
A. Sharifi ◽  
F. Keykha Akhar
Keyword(s):  
In Vitro Regeneration ◽  
Watermelon Seed
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