WRKY Transcription Factor Functions as a Transcriptional Regulator of Xylanase Inhibitor RIXI, Involved in Rice Disease Resistance to Magnaporthe oryzae

2020 ◽  
Vol 63 (3) ◽  
pp. 177-188
Author(s):  
Xiao-qi Gao ◽  
Xiang-yu Sun ◽  
Yao-yao Peng ◽  
Ying-ying Huang ◽  
Ming-Qi Liu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Disease Resistance ◽  
Magnaporthe Oryzae ◽  
Transcriptional Regulator ◽  
Wrky Transcription Factor ◽  
Rice Disease ◽  
Xylanase Inhibitor

scholarly journals Identification and characterization of genes frequently responsive to Xanthomonas oryzae pv. oryzae and Magnaporthe oryzae infections in rice

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Weiwen Kong ◽  
Li Ding ◽  
Xue Xia
Keyword(s):  
Disease Resistance ◽  
Magnaporthe Oryzae ◽  
Enrichment Analysis ◽  
Defense Responses ◽  
Resistance Mechanisms ◽  
Xanthomonas Oryzae ◽  
Transcriptional Reprogramming ◽  
Go Enrichment ◽  
Phenylpropanoid Biosynthesis ◽  
Rice Disease

Abstract Background Disease resistance is an important factor that impacts rice production. However, the mechanisms underlying rice disease resistance remain to be elucidated. Results Here, we show that a robust set of genes has been defined in rice response to the infections of Xanthomonas oryzae pv. oryzae (Xoo) and Magnaporthe oryzae (Mor). We conducted a comprehensive analysis of the available microarray data from a variety of rice samples with inoculation of Xoo and Mor. A set of 12,932 genes was identified to be regulated by Xoo and another set of 2709 Mor-regulated genes was determined. GO enrichment analysis of the regulated genes by Xoo or Mor suggested mitochondrion may be an arena for the up-regulated genes and chloroplast be another for the down-regulated genes by Xoo or Mor. Cytokinin-related processes were most frequently repressed by Xoo, while processes relevant to jasmonic acid and abscisic acid were most frequently activated by Xoo and Mor. Among genes responsive to Xoo and Mor, defense responses and diverse signaling pathways were the most frequently enriched resistance mechanisms. InterPro annotation showed the zinc finger domain family, WRKY proteins, and Myb domain proteins were the most significant transcription factors regulated by Xoo and Mor. KEGG analysis demonstrated pathways including ‘phenylpropanoid biosynthesis’, ‘biosynthesis of antibiotics’, ‘phenylalanine metabolism’, and ‘biosynthesis of secondary metabolites’ were most frequently triggered by Xoo and Mor, whereas ‘circadian rhythm-plant’ was the most frequent pathway repressed by Xoo and Mor. Conclusions The genes identified here represent a robust set of genes responsive to the infections of Xoo and Mor, which provides an overview of transcriptional reprogramming during rice defense against Xoo and Mor infections. Our study would be helpful in understanding the mechanisms of rice disease resistance.

scholarly journals Bioinformatic Prediction of SNP Markers in WRKY Sequences of Palms

CORD ◽  
2011 ◽  
Vol 27 (2) ◽  
pp. 9 ◽  
Author(s):  
Arunachalam, V
Keyword(s):  
Transcription Factor ◽  
Disease Resistance ◽  
Wrky Transcription Factor ◽  
Snp Markers ◽  
Nucleotide Polymorphisms ◽  
Transcription Factor Gene ◽  
Wrky Transcription Factors ◽  
Nucleotide Level ◽  
Single Nucleotide ◽  
Factor Gene

WRKY transcription factors are unique to plants and performs many imperative functions mainly disease resistance. In the present study we have analyzed the WRKY transcription factor gene sequences to assess the variation at single nucleotide level. We have retrieved 525 sequences of WRKY genes of palms of 334 Kb size. The sequences were purified by employing EST trimmer and were clustered into 31 contigs using CAP3. Single nucleotide Polymorphisms (SNP) and insertion/deletions (indels) were detected in contigs using the AUTOSNP software. Alternatively candidate SNP containing contigs were aligned by Clustal X to locate the SNPs. Results from these two methods were compared and false SNPs were eliminated. Finally, about 568 SNPs were found including 250 transitions, 120 transversions and 198 indels. The SNPs were seen at a frequency of 2.84/100bp in the WRKY sequences of palms. Primers were designed flanking to SNP/ indel sites with potential as markers in palms. We could obtain two novel WRKY-SNP markers (WRKY 7 and WRKY 12) which are not reported before in palms.

OsWRKY24, a blast-disease responsive transcription factor, positively regulates rice disease resistance

2018 ◽  
Vol 84 (2) ◽  
pp. 85-91
Author(s):  
Naoki Yokotani ◽  
Masahito Shikata ◽  
Hiroaki Ichikawa ◽  
Nobutaka Mitsuda ◽  
Masaru Ohme-Takagi ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Disease Resistance ◽  
Blast Disease ◽  
Rice Disease

scholarly journals PtrWRKY73, a salicylic acid-inducible poplar WRKY transcription factor, is involved in disease resistance in Arabidopsis thaliana

2015 ◽  
Vol 34 (5) ◽  
pp. 831-841 ◽  
Author(s):  
Yanjiao Duan ◽  
Yuanzhong Jiang ◽  
Shenglong Ye ◽  
Abdul Karim ◽  
Zhengyi Ling ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factor ◽  
Salicylic Acid ◽  
Disease Resistance ◽  
Wrky Transcription Factor

Overexpression of the RIXI xylanase inhibitor improves disease resistance to the fungal pathogen, Magnaporthe oryzae, in rice

2014 ◽  
Vol 120 (1) ◽  
pp. 167-177 ◽  
Author(s):  
Chunxiao Hou ◽  
Ting Lv ◽  
Yihua Zhan ◽  
Yaoyao Peng ◽  
Yingying Huang ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Magnaporthe Oryzae ◽  
Fungal Pathogen ◽  
Xylanase Inhibitor

scholarly journals In silico genome-wide identification and phylogenetic analysis of the WRKY transcription factor family in sweet orange (Citrus sinensis)

2017 ◽  
Vol 11 (06) ◽  
pp. 716-726 ◽  
Author(s):  
Eduardo Goiano da Silva ◽  
◽  
Tania Mayumi Ito ◽  
Silvia Graciele Hülse de Souza ◽  
◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Transcription Factor ◽  
In Silico ◽  
Citrus Sinensis ◽  
Sweet Orange ◽  
Wrky Transcription Factor ◽  
Transcription Factor Family ◽  
Genome Wide

scholarly journals The transcription factor OpWRKY2 positively regulates the biosynthesis of the anticancer drug camptothecin in Ophiorrhiza pumila

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Xiaolong Hao ◽  
Chenhong Xie ◽  
Qingyan Ruan ◽  
Xichen Zhang ◽  
Chao Wu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Anticancer Activity ◽  
Time Course ◽  
Indole Alkaloid ◽  
Fold Increase ◽  
Wrky Transcription Factor ◽  
Mobility Shift ◽  
Pathway Gene ◽  
Low Concentrations ◽  
Encoding Gene

AbstractThe limited bioavailability of plant-derived natural products with anticancer activity poses major challenges to the pharmaceutical industry. An example of this is camptothecin, a monoterpene indole alkaloid with potent anticancer activity that is extracted at very low concentrations from woody plants. Recently, camptothecin biosynthesis has been shown to become biotechnologically amenable in hairy-root systems of the natural producer Ophiorrhiza pumila. Here, time-course expression and metabolite analyses were performed to identify novel transcriptional regulators of camptothecin biosynthesis in O. pumila. It is shown here that camptothecin production increased over cultivation time and that the expression pattern of the WRKY transcription factor encoding gene OpWRKY2 is closely correlated with camptothecin accumulation. Overexpression of OpWRKY2 led to a more than three-fold increase in camptothecin levels. Accordingly, silencing of OpWRKY2 correlated with decreased camptothecin levels in the plant. Further detailed molecular characterization by electrophoretic mobility shift, yeast one-hybrid and dual-luciferase assays showed that OpWRKY2 directly binds and activates the central camptothecin pathway gene OpTDC. Taken together, the results of this study demonstrate that OpWRKY2 acts as a direct positive regulator of camptothecin biosynthesis. As such, a feasible strategy for the over-accumulation of camptothecin in a biotechnologically amenable system is presented.

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