This study aimed to compare estrous performance and progesterone concentration during the estrous cycle of kacang goats induced by PGF2α versus ovsynch protocol. This research used six female kacang goats. The goats were divided into two groups namely the K1 group which was induced by injecting 7.5 mg PGF2α intramuscularly in 10 days interval and K2 group which was induced by using the ovsynch method. Protocol for K2 group was as follow; at day 1, the goats were injected with 7.5 mg PGF2α; at day 8, they were injected with 50 µg GnRH; at day 15 they were injected with 7.5 mg PGF2α; at day 18 they were injected with 50 µg GnRH. Estrous were detected using male goat and visual observation. The blood samples were taken on days 7, 14, and 21 after estrous. Progesterone concentration was measured with enzyme-linked immunosorbent assay (ELISA) technique. Intensity, onset, and duration of estrous in K1 group versus K2 group were 8.33±2.08 vs 7.00±1.00; 56.00±34.12 vs 36.00±20.78 hours; and 24.00±26.15 vs 24.00±20.78 hours, respectively (p>0.05). Level of progesterone hormone on day 7, 14, and 21 for K1 vs K2 were 0.812±0.710 vs 2.369±3.351; 5.051±7.754 vs 3.091±4.385 ng/ml; and 4.173±6.692 vs 3.562±4.113 ng/mL, respectively (p>0,05). It can be concluded that the differences in synchronization protocols between PGF2α versus ovsynch do not affect the performance of estrous and the concentration of progesterone during the estrous cycle of kacang goats.
Enzyme Linked Immunosorbent Assay for Fumonisin B1 Detection in Local Corn Seeds from Baghdad-Iraq