Ekstrak Kulit Pisang Kepok (Musa paradisiaca) Menurunkan Kadar AST pada Tikus Putih yang Diinduksi Diet Tinggi Lemak

<p class="ABSTRACT">Background. Non-alcoholic fatty liver disease (NAFLD) causes many health problems in overweight person. There is an increase in the incidence of NAFLD with the increased incidence of metabolic syndrome. NAFLD is closely related to the incidence of obesity and dyslipidemia. Increased levels of AST and ALT are the most frequently performed liver function tests that can be used as a benchmark for suspicion of NAFLD. Banana peels contain flavonoids and phenolics which are antioxidants that can function as hepatoprotectors.</p><p class="ABSTRACT">Aim. Proving the effect of Kepok banana peel extract (Musa paradisiaca formatypica) on AST and ALT of Wistar rats induced high fat diet.</p><p class="ABSTRACT">Method. A laboratory experimental study with a post-test only control group design, with four treatment groups. There is one negative control group only given four weeks standard diet, one positive control group only given four weeks high-fat diet, and two treatment group where the rats were given two weeks high-fat diet then were given two weeks high-fat diet plus Kepok banana peel extract at a dose of 200 mg/kg BW and 400 mg/kg BW.</p><p class="ABSTRACT">Result. Based on the results of descriptive analysis, the highest mean AST levels were in the negative control group and the lowest was the K2 group. Meanwhile, the highest mean of ALT was in the positive control group and the lowest was the K2 group. Based on the Kruskal Wallis test, the significance of the AST variable was not different (p&gt;0.05), but the ALT variable was significantly different (p&lt;0.05). Significant differences from the Mann Whitney-U test (p&lt;0.05) were obtained between the negative control group and the positive control, the negative control group with K2 and the positive control group with the K2 group).</p><p class="ABSTRACT">Conclusion. The administration of Kepok banana peel extract (Musa paradisiaca formatypica) showed a significant decrease in ALT levels in Wistar strain white rats induced by a high-fat diet, but it was not significant for AST levels.</p>

Effectiveness of Black Honey as an Antiinflammatory Substance in Rat’s Wound Infected by Staphylococcus aureus

Background: Black honey contains higher flavonoids, phenols, and minerals, which serves as antiinflammatory agents and promote faster wound healing. Purpose: This study aimed to determine the effectiveness of black honey on macrophage cells and blood vessels in rats’ wounds infected with Staphylococcus aureus. Methods: This was an experimental study with a post-test only control group design. This study involved 24 rats divided into 4 groups. The treatment was carried out for 5 days. The rats in the K1 group were wounded (cut) and given aquadest. The rats in the K2 group were wounded (cut), infected with Staphylococcus aureus, and given aquadest. The rats in the P1 group were wounded (cut), infected with Staphylococcus aureus, and treated with 2ml of black honey topically. The rats in the P2 group were wounded (cut), infected with Staphylococcus aureus, and treated with2ml of black honey orally. Observations were made histopathologicaly using hematoxylin-eosin (HE) staining by calculating the number of macrophage and blood vessels, and further using One-Way ANOVA. Result: The number of macrophages and blood vessels between groups was significantly different. The K2 group had the highest mean number of macrophage cells and blood vessels, and the K1 group was the least. The mean number of macrophage cells in the P1 group was more than the K1 group and less than the K2 and P2 groups, both significantly. Conclusion: Black honey was effective in reducing inflammation in wounds infected by Staphylococcus aureus.

Safety and Efficacy of Vitamin K Antagonists versus Rivaroxaban in Hemodialysis Patients with Atrial Fibrillation: A Multicenter Randomized Controlled Trial

BackgroundIn patients with normal renal function or early stage CKD, the risk-benefit profile of direct oral anticoagulants (DOACs) is superior to that of vitamin K antagonists (VKAs). In patients on hemodialysis, the comparative efficacy and safety of DOACs versus VKAs are unknown.MethodsIn the Valkyrie study, 132 patients on hemodialysis with atrial fibrillation were randomized to a VKA with a target INR of 2–3, 10 mg rivaroxaban daily, or rivaroxaban and vitamin K2 for 18 months. Patients continued the originally assigned treatment and follow-up was extended for at least an additional 18 months. The primary efficacy end point was a composite of fatal and nonfatal cardiovascular events. Secondary efficacy end points were individual components of the composite outcome and all-cause death. Safety end points were life-threatening, major, and minor bleeding.ResultsMedian (IQR) follow-up was 1.88 (1.01–3.38) years. Premature, permanent discontinuation of anticoagulation occurred in 25% of patients. The primary end point occurred at a rate of 63.8 per 100 person-years in the VKA group, 26.2 per 100 person-years in the rivaroxaban group, and 21.4 per 100 person-years in the rivaroxaban and vitamin K2 group. The estimated competing risk–adjusted hazard ratio for the primary end point was 0.41 (95% CI, 0.25 to 0.68; P=0.0006) in the rivaroxaban group and 0.34 (95% CI, 0.19 to 0.61; P=0.0003) in the rivaroxaban and vitamin K2 group, compared with the VKA group. Death from any cause, cardiac death, and risk of stroke were not different between the treatment arms, but symptomatic limb ischemia occurred significantly less frequently with rivaroxaban than with VKA. After adjustment for competing risk of death, the hazard ratio for life-threatening and major bleeding compared with the VKA group was 0.39 (95% CI, 0.17 to 0.90; P=0.03) in the rivaroxaban group, 0.48 (95% CI, 0.22 to 1.08; P=0.08) in the rivaroxaban and vitamin K2 group and 0.44 (95% CI, 0.23 to 0.85; P=0.02) in the pooled rivaroxaban groups.ConclusionsIn patients on hemodialysis with atrial fibrillation, a reduced dose of rivaroxaban significantly decreased the composite outcome of fatal and nonfatal cardiovascular events and major bleeding complications compared with VKA.Clinical Trial registry name and registration number:Oral Anticoagulation in Hemodialysis, NCT03799822

PENGARUH PEMBERIAN MINYAK IKAN GINDARA (Lepidocybium flavobrunneum) TERHADAP KADAR KOLESTEROL HDL TIKUS PUTIH HIPERKOLESTEROLEMIA

Gindara fish oil contains several active ingredients that are thought to increase HDL cholesterol levels in the blood. The increase in HDL cholesterol levels is caused by gindara fish oil containing ɷ-3, ɷ-6, and ɷ-9. Gindara fish oil content is around 12-20% of body weight. A study was conducted to determine the effect of Gindara fish oil (Lepidocybium flavobrunneum) on HDL cholesterol levels in hypercholesterolemic white mice fed high-fat feed. This research was conducted experimentally using 25 male white rats (Rattus novergicus, L.), aged 2-3 months with a weight of 150-200 g, which were divided into 5 random treatment groups, namely the K1 group (normal control) were fed standard, the K2 group (positive control) was given high-fat feed for 7 days, while the P1, P2, and P3 groups were given orally gindara fish oil at a dose of 54mg / 200gBB / day, 108mg / 200gBB / day, 216mg / 200g BW / day. Each treatment consisted of 5 mice which were examined 3 times the HDL cholesterol level by the CHOD-PAP method. Data obtained from the examination were analyzed using the Crucial Wallis test p 0.05 followed by a post hoct test. with Mann-Whitney. Research Results P1, P2, and P3 groups were significantly different from K1 (p = 0.008). Groups P1, P2, and P3 were significantly different from K2 (p = 0.008). Gindara fish oil with a dose of 54mg / 200g BW / day, 108mg / 200g BW / day, 216mg / 200g BW / day each can increase HDL cholesterol levels by 38% or 15.09 mg / dl, 50% or 25.92 mg / dl, and 59% or 34.51 mg / dl.

Comparison of Estrous Performance and Progesterone Level of Kacang Goats Induced by PGF2α Versus Ovsynch Protocol

This study aimed to compare estrous performance and progesterone concentration during the estrous cycle of kacang goats induced by PGF2α versus ovsynch protocol. This research used six female kacang goats. The goats were divided into two groups namely the K1 group which was induced by injecting 7.5 mg PGF2α intramuscularly in 10 days interval and K2 group which was induced by using the ovsynch method. Protocol for K2 group was as follow; at day 1, the goats were injected with 7.5 mg PGF2α; at day 8, they were injected with 50 µg GnRH; at day 15 they were injected with 7.5 mg PGF2α; at day 18 they were injected with 50 µg GnRH. Estrous were detected using male goat and visual observation. The blood samples were taken on days 7, 14, and 21 after estrous. Progesterone concentration was measured with enzyme-linked immunosorbent assay (ELISA) technique. Intensity, onset, and duration of estrous in K1 group versus K2 group were 8.33±2.08 vs 7.00±1.00; 56.00±34.12 vs 36.00±20.78 hours; and 24.00±26.15 vs 24.00±20.78 hours, respectively (p>0.05). Level of progesterone hormone on day 7, 14, and 21 for K1 vs K2 were 0.812±0.710 vs 2.369±3.351; 5.051±7.754 vs 3.091±4.385 ng/ml; and 4.173±6.692 vs 3.562±4.113 ng/mL, respectively (p>0,05). It can be concluded that the differences in synchronization protocols between PGF2α versus ovsynch do not affect the performance of estrous and the concentration of progesterone during the estrous cycle of kacang goats.

EFEK PEMBERIAN EKSTRAK KAYU AKWAY (Drymis Sp) TERHADAP KUALITAS SPERMATOZOA MENCIT (Mus musculus L)

This research was aimed to study the effect of Akway wood (Drymis Sp) extract treatment on spermatozoa quality of mice (Mus musculus L). The extract was given orally once a day in 2 weeks. The animals were divided into three groups; one control group (K1) and two treatment groups with four replicates. K2 group was given 1.3 ml/gr body weight / day and K3 group was given 3.3 ml/ gr body weight / day. The results indicated that treatments were increased significantly (P<0.05) on acrosome integrity and viability, while on the motility, concentration and abnormality treatments had no significant effect.

The analysis of evaluation items of CPNS k1/k2 pre-service training participants group III DKI Jakarta Province 2018

The analysis of evaluation items is performed to determine the quality of the final evaluation instrument for pre-service training participants. This research is conducted using the Anates version 4.00 application with quantitative approaches and descriptive methods. The subjects of the study are CPNS K1/K2 Group III pre-service training participants of DKI Province, class 131-132 in 2018 as many as 60 people with 50 items of multiple choice evaluation items. The results of the analysis show that out of 50 items, there are 26 valid questions based on the correlation coefficient of 0.354 at the 1% significance level, and the correlation coefficient of 0.273 at the 5% significance level. Based on the results of the overall recap, 26 questions (52%) can be used. Those consist of questions 18 items (36%) that can be directly used and 8 items (16%) questions that can be used but must be corrected, while those that cannot be used or dropped are 24 items (48%).

Knotless Fixation Is Stronger and Less Variable Than Knotted Constructs in Securing a Suture Loop

Background: Historically, tendon-to-bone fixation has relied on knot tying. However, considerable variability exists in knot-tying strength among surgeons. Purpose/Hypothesis: The purpose of this study was to compare the biomechanical properties of knotted and knotless fixation and to evaluate variability among surgeons. The hypothesis was that knotless constructs would be stronger and have less variability as compared with knotted constructs. Study Design: Controlled laboratory study. Methods: A total of 34 orthopaedic surgeons participated in a laboratory study to compare knotted and knotless constructs, where 104 knotted constructs were performed with No. 2 suture, 21 knotless constructs with No. 2 suture (K2 group), and 79 knotless constructs with suture tape (KT group). Mechanical testing was performed to compare load at 3 mm of displacement, load to failure, and stiffness of each construct. Results: The mean load at 3 mm of displacement was greatest in the KT group, with significant differences among all 3 groups ( P < .001). Load to failure was significantly greater in the KT group as compared with the K2 group and the knotted group ( P < .001), but there was no difference between the K2 and knotted groups ( P ≥ .999). Stiffness and displacement were also greatest in the KT group. Based on the F test, the variance in load to failure was significantly different between the knotted and knotless constructs, with the knotted group demonstrating greater variability (SD, 94 N) than the KT (SD, 38 N) and K2 (SD, 17 N) groups ( P < .001). Conclusion: Knotless fixation with suture tape had improved biomechanical performance as compared with knots or knotless fixation with No. 2 suture. In addition, knotless fixation had less variability in biomechanical properties among multiple surgeons. Clinical Relevance: This study may be relevant for surgeons choosing between knotted and knotless constructs as well as for considerations in the design of rotator cuff repair constructs.

THE EFFECT OF RECOMBINANT VASCULAR ENDOTHELIAL GROWTH FACTOR 121 ON NITRIDE OXIDE LEVEL IN MICE (Mus musculus) MODEL OF PREECLAMPSIA

Preeclampsia is a multifactorial syndrome in pregnancy whose cause is still unknown. Several proangiogenic and antiangiogenic mediators such as Vascular Endothelial Growth Factor (VEGF) and Nitrite Oxide (NO) play important roles in preventing preeclampsia. VEGF can increase NO level that lowers maternal blood pressure, improves endothelial function and reduces placental hypoxia in preeclampsia. Recombinant VEGF 121 is expected to be an option in the prevention and treatment of preeclampsia. This experimental study used mice (Mus musculus) as the model. The objective of this study was to observe the effect of recombinant VEGF 121 in increasing the level of nitric oxide in mice (Mus musculus) model of preeclampsia. This was an experimental analytical study with Randomized Control Trial (RCT) design. The study enrolled 27 pregnant mice (Mus musculus) which met the restriction criteria divided into 3 groups. The first group (K1) were 9 normal pregnant mice. The second group (K2) were 9 pregnant mice of preeclampsia model without treatment. The third group (K3) were 9 pregnant mice of preeclampsia model receiving recombinant VEGF 121 therapy. The independent variable was the administration of recombinant VEGF 121 and the dependent variable was the serum NO level. Statistical analysis was performed by using anova statistics. NO level in the first group (K1) was 1.746±0.347, with minimum value of 1.00 µM, and maximum value of 2.28 µM, CI (1.479-2.013). NO level in second group (K2) was 1.167±0.380, with minimum value of 0.64 µM, and maximum value of 1.94 µM, CI (0.875-1.460). NO level in the third group (K3) was 2.164±0.556, with minimum value of 1.56 µM, and maximum value of 5.96 µM, CI (1.842-2.486). With anova statistical test, there were significant differences between K1 group and K2 group (p value=0.004<0.05), K1 group and K3 group (p value=0.000<0.05) as well as K2 group and K3 group (p value=0.029<0.05). In conclusion, Recombinant VEGF 121 increased the level of nitric oxide in mice (Mus musculus) model of preeclampsia significantly.

STRUKTUR HISTOLOGI TRAKEA TIKUS PUTIH (Rattus sp.) YANG TERPAPAR ASAP ROKOK SETELAH DIBERI EKSTRAK BUAH JUWET (Syzygium cumini L.)

This study aimed to determine the effect of juwet fruit extract on histological structure of rat(Rattus sp) trachea which exposed to cigarette smoke. This research used Completely Randomized Design (CRD), with four treatments: the control group (K0) treated with 0.5 % CMC–Na, (K1) group is exposed to cigarette smoke, (K2) group were given juwetfruit extract, and (K3) group is exposed to cigarette smoke and juwet fruit extracts. Each treatment consisted of 6 rats as replication. The exposure to cigarette smoke is given from an aerator pump lit cigarettes. Juwet fruit extract and 0.5 % CMC - Na was orally administered (gavage method) for 48 days. The existence of comperative descriptive observed cilia. And goblet number, high epithelium and lumen diameter Data were analyzed with ANOVA and If they were 5 % significantly different would be followed by Duncan test. Results showed that the extract of the fruit juwet significant effect on the histological structure of the trachea mice that had been exposed to smoke.